LXR alpha/NR1H3 Antibody (OTI1A5)

Novus Biologicals | Catalog # NBP2-46220

Novus Biologicals

Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Cited:

Human

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Cited:

Flow Cytometry, IF/IHC

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2A Clone # OTI1A5
View all LXR alpha/NR1H3 Primary Antibodies »

Product Specifications

Immunogen

Human recombinant protein fragment corresponding to amino acids 69-329 of human NR1H3 (NP_005684) produced in E.coli.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Theoretical MW

50.2 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

Novus Biologicals Mouse LXR alpha/NR1H3 Antibody (OTI1A5) (NBP2-46220) is a monoclonal antibody validated for use in IHC, WB and Flow. Anti-LXR alpha/NR1H3 Antibody: Cited in 1 publication. All Novus Biologicals antibodies are covered by our 100% guarantee.

Scientific Data Images for LXR alpha/NR1H3 Antibody (OTI1A5)

Western Blot: LXR alpha/NR1H3 Antibody (OTI1A5) [NBP2-46220]

Western Blot: LXR alpha/NR1H3 Antibody (OTI1A5) [NBP2-46220]

Western Blot: LXR alpha/NR1H3 Antibody (1A5) [NBP2-46220] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY LXR.
Immunohistochemistry: LXR alpha/NR1H3 Antibody (OTI1A5) [NBP2-46220]

Immunohistochemistry: LXR alpha/NR1H3 Antibody (OTI1A5) [NBP2-46220]

Immunohistochemistry: LXR alpha/NR1H3 Antibody (1A5) [NBP2-46220] - Analysis of Human tonsil tissue. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 min)
Immunohistochemistry: LXR alpha/NR1H3 Antibody (OTI1A5) [NBP2-46220]

Immunohistochemistry: LXR alpha/NR1H3 Antibody (OTI1A5) [NBP2-46220]

Immunohistochemistry: LXR alpha/NR1H3 Antibody (1A5) [NBP2-46220] - Analysis of Human Kidney tissue. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 min)(1:150)
Immunohistochemistry: LXR alpha/NR1H3 Antibody (OTI1A5) [NBP2-46220]

Immunohistochemistry: LXR alpha/NR1H3 Antibody (OTI1A5) [NBP2-46220]

Immunohistochemistry: LXR alpha/NR1H3 Antibody (1A5) [NBP2-46220] - Analysis of Carcinoma of Human kidney tissue. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 min)(1:150)
Immunohistochemistry: LXR alpha/NR1H3 Antibody (OTI1A5) [NBP2-46220]

Immunohistochemistry: LXR alpha/NR1H3 Antibody (OTI1A5) [NBP2-46220]

Immunohistochemistry: LXR alpha/NR1H3 Antibody (1A5) [NBP2-46220] - Analysis of Carcinoma of Human lung tissue.(Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 min)(1:150)
Immunohistochemistry: LXR alpha/NR1H3 Antibody (OTI1A5) [NBP2-46220]

Immunohistochemistry: LXR alpha/NR1H3 Antibody (OTI1A5) [NBP2-46220]

Immunohistochemistry: LXR alpha/NR1H3 Antibody (1A5) [NBP2-46220] - Analysis of Human Ovary tissue. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 min)(1:150)
Immunohistochemistry: LXR alpha/NR1H3 Antibody (OTI1A5) [NBP2-46220]

Immunohistochemistry: LXR alpha/NR1H3 Antibody (OTI1A5) [NBP2-46220]

Immunohistochemistry: LXR alpha/NR1H3 Antibody (1A5) [NBP2-46220] - Analysis of Adenocarcinoma of Human ovary tissue. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 min)(1:150)
Immunohistochemistry: LXR alpha/NR1H3 Antibody (OTI1A5) [NBP2-46220]

Immunohistochemistry: LXR alpha/NR1H3 Antibody (OTI1A5) [NBP2-46220]

Immunohistochemistry: LXR alpha/NR1H3 Antibody (1A5) [NBP2-46220] - Analysis of Human pancreas tissue. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 min)(1:150)
Immunohistochemistry: LXR alpha/NR1H3 Antibody (OTI1A5) [NBP2-46220]

Immunohistochemistry: LXR alpha/NR1H3 Antibody (OTI1A5) [NBP2-46220]

Immunohistochemistry: LXR alpha/NR1H3 Antibody (1A5) [NBP2-46220] - Analysis of Human thyroid tissue. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 min)(1:150)
Immunohistochemistry: LXR alpha/NR1H3 Antibody (OTI1A5) [NBP2-46220]

Immunohistochemistry: LXR alpha/NR1H3 Antibody (OTI1A5) [NBP2-46220]

Immunohistochemistry: LXR alpha/NR1H3 Antibody (1A5) [NBP2-46220] - Analysis of Carcinoma of Human thyroid tissue. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 min)(1:150)
Immunohistochemistry: LXR alpha/NR1H3 Antibody (OTI1A5) [NBP2-46220]

Immunohistochemistry: LXR alpha/NR1H3 Antibody (OTI1A5) [NBP2-46220]

Immunohistochemistry: LXR alpha/NR1H3 Antibody (1A5) [NBP2-46220] - Analysis of Human endometrium tissue. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 min)
Immunohistochemistry: LXR alpha/NR1H3 Antibody (OTI1A5) [NBP2-46220]

Immunohistochemistry: LXR alpha/NR1H3 Antibody (OTI1A5) [NBP2-46220]

Immunohistochemistry: LXR alpha/NR1H3 Antibody (1A5) [NBP2-46220] - Analysis of Adenocarcinoma of Human endometrium tissue. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 min)(1:150)
Immunohistochemistry: LXR alpha/NR1H3 Antibody (OTI1A5) [NBP2-46220]

Immunohistochemistry: LXR alpha/NR1H3 Antibody (OTI1A5) [NBP2-46220]

Immunohistochemistry: LXR alpha/NR1H3 Antibody (1A5) [NBP2-46220] - Analysis of Human prostate tissue. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 min)
Immunohistochemistry: LXR alpha/NR1H3 Antibody (OTI1A5) [NBP2-46220]

Immunohistochemistry: LXR alpha/NR1H3 Antibody (OTI1A5) [NBP2-46220]

Immunohistochemistry: LXR alpha/NR1H3 Antibody (1A5) [NBP2-46220] - Analysis of Carcinoma of Human prostate tissue. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 min)(1:150)
Immunohistochemistry: LXR alpha/NR1H3 Antibody (OTI1A5) [NBP2-46220]

Immunohistochemistry: LXR alpha/NR1H3 Antibody (OTI1A5) [NBP2-46220]

Immunohistochemistry: LXR alpha/NR1H3 Antibody (1A5) [NBP2-46220] - Analysis of Human bladder tissue. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 min)(1:150)
Immunohistochemistry: LXR alpha/NR1H3 Antibody (OTI1A5) [NBP2-46220]

Immunohistochemistry: LXR alpha/NR1H3 Antibody (OTI1A5) [NBP2-46220]

Immunohistochemistry: LXR alpha/NR1H3 Antibody (1A5) [NBP2-46220] - Analysis of Human lymph node tissue. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 min)
Immunohistochemistry: LXR alpha/NR1H3 Antibody (OTI1A5) [NBP2-46220]

Immunohistochemistry: LXR alpha/NR1H3 Antibody (OTI1A5) [NBP2-46220]

Immunohistochemistry: LXR alpha/NR1H3 Antibody (1A5) [NBP2-46220] - Analysis of Human lymphoma tissue.(Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 min)

Applications for LXR alpha/NR1H3 Antibody (OTI1A5)

Application
Recommended Usage

Immunohistochemistry

1:150

Western Blot

1:2000

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.3), 1.0% BSA and 50% Glycerol

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: LXR alpha/NR1H3

Liver X Receptors (LXRs) are oxysterol activated nuclear receptors which are involved in the regulation of cholesterol metabolism and bile acids. Two known isoforms, alpha and beta, are differentially expressed. The alpha isoform is predominantly expressed in liver, whereas the beta isoform expression has been shown to be ubiquitous.

LXR antibodies are useful for lipid and cholesterol metabolism studies.

Long Name

Liver X Receptor alpha

Alternate Names

NR1H3, RLD-1

Gene Symbol

NR1H3

Additional LXR alpha/NR1H3 Products

Product Documents for LXR alpha/NR1H3 Antibody (OTI1A5)

Certificate of Analysis

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Product Specific Notices for LXR alpha/NR1H3 Antibody (OTI1A5)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for LXR alpha/NR1H3 Antibody (OTI1A5)

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Protocols

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