Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2A Clone # OTI1A5

Format

Azide and BSA Free
View all LXR alpha/NR1H3 Primary Antibodies »

Product Specifications

Immunogen

Human recombinant protein fragment corresponding to amino acids 69-329 of human NR1H3 (NP_005684) produced in E.coli.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Theoretical MW

50.2 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

Novus Biologicals Mouse LXR alpha/NR1H3 Antibody (OTI1A5) - Azide and BSA Free (NBP2-46220) is a monoclonal antibody validated for use in IHC and WB. All Novus Biologicals antibodies are covered by our 100% guarantee.

Scientific Data Images for LXR alpha/NR1H3 Antibody (OTI1A5) - Azide and BSA Free

Western Blot: LXR alpha/NR1H3 Antibody (OTI1A5)Azide and BSA Free [NBP2-72550]

Western Blot: LXR alpha/NR1H3 Antibody (OTI1A5)Azide and BSA Free [NBP2-72550]

Western Blot: LXR alpha/NR1H3 Antibody (OTI1A5) - Azide and BSA Free [NBP2-72550] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY LXR.
Immunohistochemistry: LXR alpha/NR1H3 Antibody (OTI1A5) - Azide and BSA Free [NBP2-72550]

Immunohistochemistry: LXR alpha/NR1H3 Antibody (OTI1A5) - Azide and BSA Free [NBP2-72550]

Immunohistochemistry: LXR alpha/NR1H3 Antibody (OTI1A5) - Azide and BSA Free [NBP2-72550] - Analysis of Human thyroid tissue. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 min)(1:150)
Immunohistochemistry: LXR alpha/NR1H3 Antibody (OTI1A5) - Azide and BSA Free [NBP2-72550]

Immunohistochemistry: LXR alpha/NR1H3 Antibody (OTI1A5) - Azide and BSA Free [NBP2-72550]

Immunohistochemistry: LXR alpha/NR1H3 Antibody (OTI1A5) - Azide and BSA Free [NBP2-72550] - Analysis of Adenocarcinoma of Human endometrium tissue. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 min)(1:150)
Immunohistochemistry: LXR alpha/NR1H3 Antibody (OTI1A5) - Azide and BSA Free [NBP2-72550]

Immunohistochemistry: LXR alpha/NR1H3 Antibody (OTI1A5) - Azide and BSA Free [NBP2-72550]

Immunohistochemistry: LXR alpha/NR1H3 Antibody (OTI1A5) - Azide and BSA Free [NBP2-72550] - Analysis of Adenocarcinoma of Human ovary tissue. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 min)(1:150)
Immunohistochemistry: LXR alpha/NR1H3 Antibody (OTI1A5) - Azide and BSA Free [NBP2-72550]

Immunohistochemistry: LXR alpha/NR1H3 Antibody (OTI1A5) - Azide and BSA Free [NBP2-72550]

Immunohistochemistry: LXR alpha/NR1H3 Antibody (OTI1A5) - Azide and BSA Free [NBP2-72550] - Analysis of Carcinoma of Human kidney tissue. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 min)(1:150)
Immunohistochemistry: LXR alpha/NR1H3 Antibody (OTI1A5) - Azide and BSA Free [NBP2-72550]

Immunohistochemistry: LXR alpha/NR1H3 Antibody (OTI1A5) - Azide and BSA Free [NBP2-72550]

Immunohistochemistry: LXR alpha/NR1H3 Antibody (OTI1A5) - Azide and BSA Free [NBP2-72550] - Analysis of Carcinoma of Human lung tissue.(Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 min)(1:150)
Immunohistochemistry: LXR alpha/NR1H3 Antibody (OTI1A5) - Azide and BSA Free [NBP2-72550]

Immunohistochemistry: LXR alpha/NR1H3 Antibody (OTI1A5) - Azide and BSA Free [NBP2-72550]

Immunohistochemistry: LXR alpha/NR1H3 Antibody (OTI1A5) - Azide and BSA Free [NBP2-72550] - Analysis of Carcinoma of Human prostate tissue. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 min)(1:150)
Immunohistochemistry: LXR alpha/NR1H3 Antibody (OTI1A5) - Azide and BSA Free [NBP2-72550]

Immunohistochemistry: LXR alpha/NR1H3 Antibody (OTI1A5) - Azide and BSA Free [NBP2-72550]

Immunohistochemistry: LXR alpha/NR1H3 Antibody (OTI1A5) - Azide and BSA Free [NBP2-72550] - Analysis of Carcinoma of Human thyroid tissue. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 min)(1:150)
Immunohistochemistry: LXR alpha/NR1H3 Antibody (OTI1A5) - Azide and BSA Free [NBP2-72550]

Immunohistochemistry: LXR alpha/NR1H3 Antibody (OTI1A5) - Azide and BSA Free [NBP2-72550]

Immunohistochemistry: LXR alpha/NR1H3 Antibody (OTI1A5) - Azide and BSA Free [NBP2-72550] - Analysis of Human bladder tissue. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 min)(1:150)
Immunohistochemistry: LXR alpha/NR1H3 Antibody (OTI1A5) - Azide and BSA Free [NBP2-72550]

Immunohistochemistry: LXR alpha/NR1H3 Antibody (OTI1A5) - Azide and BSA Free [NBP2-72550]

Immunohistochemistry: LXR alpha/NR1H3 Antibody (OTI1A5) - Azide and BSA Free [NBP2-72550] - Analysis of Human endometrium tissue. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 min)
Immunohistochemistry: LXR alpha/NR1H3 Antibody (OTI1A5) - Azide and BSA Free [NBP2-72550]

Immunohistochemistry: LXR alpha/NR1H3 Antibody (OTI1A5) - Azide and BSA Free [NBP2-72550]

Immunohistochemistry: LXR alpha/NR1H3 Antibody (OTI1A5) - Azide and BSA Free [NBP2-72550] - Analysis of Human Kidney tissue. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 min)(1:150)
Immunohistochemistry: LXR alpha/NR1H3 Antibody (OTI1A5) - Azide and BSA Free [NBP2-72550]

Immunohistochemistry: LXR alpha/NR1H3 Antibody (OTI1A5) - Azide and BSA Free [NBP2-72550]

Immunohistochemistry: LXR alpha/NR1H3 Antibody (OTI1A5) - Azide and BSA Free [NBP2-72550] - Analysis of Human lymph node tissue. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 min)
Immunohistochemistry: LXR alpha/NR1H3 Antibody (OTI1A5) - Azide and BSA Free [NBP2-72550]

Immunohistochemistry: LXR alpha/NR1H3 Antibody (OTI1A5) - Azide and BSA Free [NBP2-72550]

Immunohistochemistry: LXR alpha/NR1H3 Antibody (OTI1A5) - Azide and BSA Free [NBP2-72550] - Analysis of Human lymphoma tissue.(Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 min)
Immunohistochemistry: LXR alpha/NR1H3 Antibody (OTI1A5) - Azide and BSA Free [NBP2-72550]

Immunohistochemistry: LXR alpha/NR1H3 Antibody (OTI1A5) - Azide and BSA Free [NBP2-72550]

Immunohistochemistry: LXR alpha/NR1H3 Antibody (OTI1A5) - Azide and BSA Free [NBP2-72550] - Analysis of Human Ovary tissue. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 min)(1:150)
Immunohistochemistry: LXR alpha/NR1H3 Antibody (OTI1A5) - Azide and BSA Free [NBP2-72550]

Immunohistochemistry: LXR alpha/NR1H3 Antibody (OTI1A5) - Azide and BSA Free [NBP2-72550]

Immunohistochemistry: LXR alpha/NR1H3 Antibody (OTI1A5) - Azide and BSA Free [NBP2-72550] - Analysis of Human pancreas tissue. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 min)(1:150)
Immunohistochemistry: LXR alpha/NR1H3 Antibody (OTI1A5) - Azide and BSA Free [NBP2-72550]

Immunohistochemistry: LXR alpha/NR1H3 Antibody (OTI1A5) - Azide and BSA Free [NBP2-72550]

Immunohistochemistry: LXR alpha/NR1H3 Antibody (OTI1A5) - Azide and BSA Free [NBP2-72550] - Analysis of Human prostate tissue. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 min)
Immunohistochemistry: LXR alpha/NR1H3 Antibody (OTI1A5) - Azide and BSA Free [NBP2-72550]

Immunohistochemistry: LXR alpha/NR1H3 Antibody (OTI1A5) - Azide and BSA Free [NBP2-72550]

Immunohistochemistry: LXR alpha/NR1H3 Antibody (OTI1A5) - Azide and BSA Free [NBP2-72550] - Analysis of Human tonsil tissue. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 min)

Applications for LXR alpha/NR1H3 Antibody (OTI1A5) - Azide and BSA Free

Application
Recommended Usage

Immunohistochemistry

1:150

Western Blot

1:2000

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Reconstitution

we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process.

Formulation

Lyophilized from PBS (pH 7.3) with 8% Trehalose

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

LYOPH mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷

Background: LXR alpha/NR1H3

Liver X Receptors (LXRs) are oxysterol activated nuclear receptors which are involved in the regulation of cholesterol metabolism and bile acids. Two known isoforms, alpha and beta, are differentially expressed. The alpha isoform is predominantly expressed in liver, whereas the beta isoform expression has been shown to be ubiquitous.

LXR antibodies are useful for lipid and cholesterol metabolism studies.

Long Name

Liver X Receptor alpha

Alternate Names

NR1H3, RLD-1

Gene Symbol

NR1H3

Additional LXR alpha/NR1H3 Products

Product Documents for LXR alpha/NR1H3 Antibody (OTI1A5) - Azide and BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for LXR alpha/NR1H3 Antibody (OTI1A5) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customer Reviews for LXR alpha/NR1H3 Antibody (OTI1A5) - Azide and BSA Free

There are currently no reviews for this product. Be the first to review LXR alpha/NR1H3 Antibody (OTI1A5) - Azide and BSA Free and earn rewards!

Have you used LXR alpha/NR1H3 Antibody (OTI1A5) - Azide and BSA Free?

Submit a review and receive an Amazon gift card!

$25/€18/£15/$25CAN/¥2500 Yen for a review with an image

$10/€7/£6/$10CAN/¥1110 Yen for a review without an image

Submit a review
Amazon Gift Card

Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs

No product specific FAQs exist for this product.

View all FAQs for Antibodies