Ly-6H Antibody (3E10) - Azide and BSA Free

Novus Biologicals | Catalog # H00004062-M01

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Cited:

Human, Rat

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, ELISA, Immunoprecipitation

Cited:

Western Blot, Immunoprecipitation, IF/IHC

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # 3E10

Format

Azide and BSA Free
View all Ly-6H Primary Antibodies »

Product Specifications

Immunogen

LY6H (AAH28894, 26 a.a. ~ 140 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. LWCQDCTLTTNSSHCTPKQCQPSDTVCASVRITDPSSSRKDHSVNKMCASSCDFVKRHFFSDYLMGFINSGILKVDVDCCEKDLCNGAAGAGHSPWALAGGLLLSLGPALLWAGP

Reactivity Notes

Rat reactivity reported in scientific literature (PMID: 23860910 and 25716842).

Localization

Cell membrane. Lipid-anchor, GPI-anchor.

Specificity

LY6H - lymphocyte antigen 6 complex, locus H

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Description

Quality control test: Antibody Reactive Against Recombinant Protein.

Scientific Data Images for Ly-6H Antibody (3E10) - Azide and BSA Free

Western Blot: Ly-6H Antibody (3E10) [H00004062-M01]

Western Blot: Ly-6H Antibody (3E10) [H00004062-M01]

Ly-6H-Antibody-3E10-Western-Blot-H00004062-M01-img0007.jpg
Immunohistochemistry-Paraffin: Ly-6H Antibody (3E10) [H00004062-M01]

Immunohistochemistry-Paraffin: Ly-6H Antibody (3E10) [H00004062-M01]

Immunohistochemistry-Paraffin: Ly-6H Antibody (3E10) [H00004062-M01] - Analysis of monoclonal antibody to LY6H on formalin-fixed paraffin-embedded human tonsil tissue. Antibody concentration 2 ug/ml.
Western Blot: Ly-6H Antibody (3E10) [H00004062-M01]

Western Blot: Ly-6H Antibody (3E10) [H00004062-M01]

Western Blot: Ly-6H Antibody (3E10) [H00004062-M01] - Ly-6H in mouse Ly-6H proteins using anti-Ly-6H antibody. Image from verified customer review.
Western Blot: Ly-6H Antibody (3E10) [H00004062-M01]

Western Blot: Ly-6H Antibody (3E10) [H00004062-M01]

Western Blot: Ly-6H Antibody (3E10) [H00004062-M01] - LY6H monoclonal antibody (M01), clone 3E10 Analysis of LY6H expression in K-562.
Ly-6H Antibody (3E10)

Western Blot: Ly-6H Antibody (3E10) [H00004062-M01] -

Western Blot: Ly-6H Antibody (3E10) [H00004062-M01] - Short‐term nicotine exposure does not alter Lypd6, Lynx1, or Ly6H levels in the brain. Lypd6, Lynx1, Ly6H, & beta 2 nAChR subunit protein levels were analyzed in (a) frontal cortex (FC) & hippocampal (HIP) tissue from rats administered nicotine (0.4 mg/kg s.c., twice daily) or vehicle (0.9% saline) for 7 days from day 8–14 or (b) 54–60 (n = 8). (c & d) Representative images of western blots summarized in (a) & (b), respectively. Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/27344019), licensed under a CC-BY license. Not internally tested by Novus Biologicals.
Ly-6H Antibody (3E10)

Western Blot: Ly-6H Antibody (3E10) [H00004062-M01] -

Western Blot: Ly-6H Antibody (3E10) [H00004062-M01] - Short‐term nicotine exposure does not alter Lypd6, Lynx1, or Ly6H levels in the brain. Lypd6, Lynx1, Ly6H, & beta 2 nAChR subunit protein levels were analyzed in (a) frontal cortex (FC) & hippocampal (HIP) tissue from rats administered nicotine (0.4 mg/kg s.c., twice daily) or vehicle (0.9% saline) for 7 days from day 8–14 or (b) 54–60 (n = 8). (c & d) Representative images of western blots summarized in (a) & (b), respectively. Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/27344019), licensed under a CC-BY license. Not internally tested by Novus Biologicals.
Ly-6H Antibody (3E10)

Western Blot: Ly-6H Antibody (3E10) [H00004062-M01] -

Western Blot: Ly-6H Antibody (3E10) [H00004062-M01] - Short‐term nicotine exposure does not alter Lypd6, Lynx1, or Ly6H levels in the brain. Lypd6, Lynx1, Ly6H, & beta 2 nAChR subunit protein levels were analyzed in (a) frontal cortex (FC) & hippocampal (HIP) tissue from rats administered nicotine (0.4 mg/kg s.c., twice daily) or vehicle (0.9% saline) for 7 days from day 8–14 or (b) 54–60 (n = 8). (c & d) Representative images of western blots summarized in (a) & (b), respectively. Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/27344019), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Applications for Ly-6H Antibody (3E10) - Azide and BSA Free

Application
Recommended Usage

Immunohistochemistry

1:10-1:500

Immunohistochemistry-Paraffin

1:10-1:500

Immunoprecipitation

1:10-1:500

Western Blot

1:500
Application Notes
This antibody reactivity against cell lysate and recombinant protein for WB. It has been used for IHC-P and ELISA. Use in ICC reported in scientific literature (PMID: 25716842). Use in immunoprecipitation reported in scientific literature (PMID: 25716842). Use in Immunohistochemistry reported in scientific literature (PMID: 25716842).

Reviewed Applications

Read 1 review rated 5 using H00004062-M01 in the following applications:

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

PBS (pH 7.4)

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: Ly-6H

LY6H (Lymphocyte antigen 6 complex, Locus H) is a member of the LY6 antigens family of glycosylphosphatidylinositol-anchored cell surface glycoproteins.

Alternate Names

Ly-6H, lymphocyte antigen 6 complex, locus H, lymphocyte antigen 6H, NMLY6

Entrez Gene IDs

4062 (Human)

Gene Symbol

LY6H

OMIM

603625 (Human)

UniProt

AAH28894 (Human)

Additional Ly-6H Products

Product Documents for Ly-6H Antibody (3E10) - Azide and BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for Ly-6H Antibody (3E10) - Azide and BSA Free

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for Ly-6H Antibody (3E10) - Azide and BSA Free

Customer Reviews for Ly-6H Antibody (3E10) - Azide and BSA Free (1)

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  • Name: Anonymous
    Application: Western Blot
    Sample Tested:
    Species: Mouse
    Verified Customer | Posted 11/24/2014
    ly6 antibody on mouse
    Ly-6H Antibody (3E10) - Azide and BSA Free H00004062-M01

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Protocols

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