Lysine (K)-specific Demethylase 3A/KDM3A/JMJD1A Antibody - BSA Free

Novus Biologicals | Catalog # NB100-77282

Novus Biologicals

Key Product Details

Validated by

Knockout/Knockdown, Independent Antibodies

Species Reactivity

Validated:

Human, Mouse

Cited:

Human, Mouse, Rat

Applications

Validated:

Knockout Validated, Immunohistochemistry, Western Blot, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation, Chromatin Immunoprecipitation (ChIP)

Cited:

Western Blot, Flow Cytometry, Immunoprecipitation, Chemotaxis, IF/IHC

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all Lysine (K)-specific Demethylase 3A/KDM3A Primary Antibodies »

Product Specifications

Immunogen

The immunogen recognized by this antibody maps to a region between residue 1271 and 1321 of human jumonji domain containing 1A (JmjC domain-containing histone demethylation protein 2A) using the numbering given in entry NP_060903.2 (GeneID 55818).

Reactivity Notes

Mouse reactivity reported in scientific literature (PMID: 24554764).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Lysine (K)-specific Demethylase 3A/KDM3A/JMJD1A Antibody - BSA Free

Knockout Validated: Lysine (K)-specific Demethylase 3A/KDM3A/JMJD1A Antibody [NB100-77282]

Western Blot: Lysine (K)-specific Demethylase 3A/KDM3A/JMJD1A Antibody [NB100-77282]

Lysine-K-specific-Demethylase-3A-KDM3A-JMJD1A-Antibody-Knockout-Validated-NB100-77282-img0003.jpg
Western Blot: Lysine (K)-specific Demethylase 3A/KDM3A/JMJD1A Antibody [NB100-77282]

Western Blot: Lysine (K)-specific Demethylase 3A/KDM3A/JMJD1A Antibody [NB100-77282]

Western Blot: Lysine (K)-specific Demethylase 3A/KDM3A/JMJD1A Antibody [NB100-77282] - Detection of Human JMJD1A/JHDM2A on HeLa whole cell lysate using NB100-77282. Lysine (K)-specific Demethylase 3A/KDM3A/JMJD1A was also immunoprecipitated by rabbit anti-Lysine (K)-specific Demethylase 3A/KDM3A/JMJD1A antibody NB100-77281.
Immunocytochemistry/ Immunofluorescence: Lysine (K)-specific Demethylase 3A/KDM3A/JMJD1A Antibody [NB100-77282]

Immunocytochemistry/ Immunofluorescence: Lysine (K)-specific Demethylase 3A/KDM3A/JMJD1A Antibody [NB100-77282]

Immunocytochemistry/Immunofluorescence: Lysine (K)-specific Demethylase 3A/KDM3A/JMJD1A Antibody [NB100-77282] - Human cancer cells stained with NB100-77282 and Alexa Fluor 488 conjugated goat-anti-rabbit secondary antibody. DNA stained with Hoechst 33342. Staining shows KDM3A protein in mixed nuclear and cytoplasmic location. ICC/IF image submitted by a verified customer review.

Applications for Lysine (K)-specific Demethylase 3A/KDM3A/JMJD1A Antibody - BSA Free

Application
Recommended Usage

Immunoprecipitation

2 - 5 ug/mg lysate

Western Blot

1:2000 - 1:10000
Application Notes
Use in chromatin immunoprecipitation reported in scientific literature (PMID: 19624751). Use in Immunohistochemistry reported in scientific literature (PMID: 30405805). This Lysine (K)-specific Demethylase 3A/KDM3A/JMJD1A Antibody is validated for ICC/IF from a verified customer review.

Reviewed Applications

Read 1 review rated 5 using NB100-77282 in the following applications:

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris-Citrate/Phosphate (pH 7.0 - 8.0)

Format

BSA Free

Preservative

0.09% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: Lysine (K)-specific Demethylase 3A/KDM3A

This gene encodes a zinc finger protein that contains a jumonji domain.

Alternate Names

JHDM2A, JMJD1A, KDM3A, KIAA0742, Lysine (K)specific Demethylase 3A, TSGA

Entrez Gene IDs

55818 (Human)

Gene Symbol

KDM3A

UniProt

Q9Y4C1

Additional Lysine (K)-specific Demethylase 3A/KDM3A Products

Product Documents for Lysine (K)-specific Demethylase 3A/KDM3A/JMJD1A Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for Lysine (K)-specific Demethylase 3A/KDM3A/JMJD1A Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

Epigenetics

Citations for Lysine (K)-specific Demethylase 3A/KDM3A/JMJD1A Antibody - BSA Free

Customer Reviews for Lysine (K)-specific Demethylase 3A/KDM3A/JMJD1A Antibody - BSA Free (1)

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Customer Images

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  • Lysine (K)-specific Demethylase 3A/KDM3A/JMJD1A Antibody
    Name: Anonymous
    Application: Indirect Immunofluorescence
    Sample Tested: Cancer Cells
    Species: Human
    Verified Customer | Posted 04/27/2021
    Human cancer cells stained with NB100-77282 and Alexa-488 conjugated goat-anti-rabbit secondary antibody. DNA in stained with Hoechst 33342. Staining Shows KDM3A Protein in mixed nuclear and cytoplasmic location.
    Lysine (K)-specific Demethylase 3A/KDM3A/JMJD1A Antibody - BSA Free NB100-77282
    Bio-Techne Response
    This review was submitted through the legacy Novus Innovators Program, reflecting a new species or application tested on a primary antibody.

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Protocols

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FAQs for Lysine (K)-specific Demethylase 3A/KDM3A/JMJD1A Antibody - BSA Free

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  • Q: Would you please let me know if NB100-77282 is validated for ChIP?

    A: NB100-77282 hasn't been tested for use in ChIP. Theoretically this antibody can work for ChIP if you get successful cross-linking of JMJD1A to K9 on H3. There should be enough JMJD1A present to be able to capture the quick de-methylation step and you'd only need a couple DNA strands to IP to be able to amplify by PCR. This antibody should work for ChIP but it will most likely IP a low amount of DNA and we can not guarantee it for use in ChIP.

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