Lysine (K)-specific Demethylase 6B/KDM6B/JMJD3 Antibody - BSA Free

Novus Biologicals | Catalog # NBP2-82107

Novus Biologicals
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Key Product Details

Validated by

Independent Antibodies

Species Reactivity

Human, Mouse, Rat

Applications

Western Blot, ELISA, Immunocytochemistry, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all Lysine (K)-specific Demethylase 6B/KDM6B Primary Antibodies »

Product Specifications

Immunogen

Antibody was raised against a peptide corresponding to 16 amino acids near the carboxy terminus of human Lysine (K)-specific Demethylase 6B/KDM6B/JMJD3. The immunogen is located within amino acids 1370-1420 of Lysine (K)-specific Demethylase 6B/KDM6B/JMJD3.

Specificity

This antibody is specific for Lysine (K)-specific Demethylase 6B/KDM6B/JMJD3 and will not recognize other JMJD proteins. Human Lysine (K)-specific Demethylase 6B/KDM6B/JMJD3 has 2 isoforms, including isoform 1 (1682aa, 181kD) and isoform 2 (1643aa, 177kD). Mouse Lysine (K)-specific Demethylase 6B/KDM6B/JMJD3 has one isoform (1641aa, 176kD) and rat Lysine (K)-specific Demethylase 6B/KDM6B/JMJD3 also has one isoform (1356aa, 146kD). NBP2-82107 can detect human, mouse and rat.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

180 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Lysine (K)-specific Demethylase 6B/KDM6B/JMJD3 Antibody - BSA Free

Western Blot: Lysine (K)-specific Demethylase 6B/KDM6B/JMJD3 AntibodyBSA Free [NBP2-82107]

Western Blot: Lysine (K)-specific Demethylase 6B/KDM6B/JMJD3 AntibodyBSA Free [NBP2-82107]

Western Blot: Lysine (K)-specific Demethylase 6B/KDM6B/JMJD3 Antibody [NBP2-82107] - Western Blot Validation in HepG2 Cell Lysate. Loading: 15 ug of lysates per lane. Antibodies: Lysine (K)-specific Demethylase 6B/KDM6B/JMJD3, NBP2-82107, 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution. Lane 1: 0.25 ug/mL Lane 2: 0.5 ug/mL Lane 3: 0.25 ug/mL with blocking peptide
Western Blot: Lysine (K)-specific Demethylase 6B/KDM6B/JMJD3 AntibodyBSA Free [NBP2-82107]

Western Blot: Lysine (K)-specific Demethylase 6B/KDM6B/JMJD3 AntibodyBSA Free [NBP2-82107]

Western Blot: Lysine (K)-specific Demethylase 6B/KDM6B/JMJD3 Antibody [NBP2-82107] - Western Blot Validation in Human, Mouse, and Rat Cell Lines. Loading: 15 ug of lysates per lane. Antibodies: Lysine (K)-specific Demethylase 6B/KDM6B/JMJD3 [NBP2-82107], (1 ug/ml), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
Western Blot: Lysine (K)-specific Demethylase 6B/KDM6B/JMJD3 AntibodyBSA Free [NBP2-82107]

Western Blot: Lysine (K)-specific Demethylase 6B/KDM6B/JMJD3 AntibodyBSA Free [NBP2-82107]

Western Blot: Lysine (K)-specific Demethylase 6B/KDM6B/JMJD3 Antibody [NBP2-82107] - Independent Antibody Validation (IAV) via Protein Expression Profile in Cell Lines.Loading: 15 ug of lysates per lane. Antibodies: Lysine (K)-specific Demethylase 6B/KDM6B/JMJD3 NBP2-82107 (1 ug/mL), JMJD3 from Company B, (2 ug/mL) and beta-actin NBP1-76692 (1 ug/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
Western Blot: Lysine (K)-specific Demethylase 6B/KDM6B/JMJD3 AntibodyBSA Free [NBP2-82107]

Western Blot: Lysine (K)-specific Demethylase 6B/KDM6B/JMJD3 AntibodyBSA Free [NBP2-82107]

Western Blot: Lysine (K)-specific Demethylase 6B/KDM6B/JMJD3 Antibody [NBP2-82107] - Western Blot Validation in HeLa Cell Lysate. Loading: 15 ug of lysates per lane. Antibodies: Lysine (K)-specific Demethylase 6B/KDM6B/JMJD3, NBP2-82107 (0.25 ug/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
Western Blot: Lysine (K)-specific Demethylase 6B/KDM6B/JMJD3 AntibodyBSA Free [NBP2-82107]

Western Blot: Lysine (K)-specific Demethylase 6B/KDM6B/JMJD3 AntibodyBSA Free [NBP2-82107]

Western Blot: Lysine (K)-specific Demethylase 6B/KDM6B/JMJD3 Antibody [NBP2-82107] - Western Blot Validation in K562 Cell Lysate. Loading: 15 ug of lysates per lane. Antibodies: Lysine (K)-specific Demethylase 6B/KDM6B/JMJD3, NBP2-82107 (0.5 ug/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution. Lane 1: in the absence and Lane 2: in the presence of blocking peptide.
Western Blot: Lysine (K)-specific Demethylase 6B/KDM6B/JMJD3 AntibodyBSA Free [NBP2-82107]

Western Blot: Lysine (K)-specific Demethylase 6B/KDM6B/JMJD3 AntibodyBSA Free [NBP2-82107]

Western Blot: Lysine (K)-specific Demethylase 6B/KDM6B/JMJD3 Antibody [NBP2-82107] - Western Blot Validation in Mouse A-20 Cell Lysate. Loading: 15 ug of lysates per lane. Antibodies: Lysine (K)-specific Demethylase 6B/KDM6B/JMJD3, NBP2-82107 (0.5 ug/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution. Lane 1: in the absence and Lane 2: in the presence of blocking peptide
Immunocytochemistry/ Immunofluorescence: Lysine (K)-specific Demethylase 6B/KDM6B/JMJD3 Antibody - BSA Free [NBP2-82107]

Immunocytochemistry/ Immunofluorescence: Lysine (K)-specific Demethylase 6B/KDM6B/JMJD3 Antibody - BSA Free [NBP2-82107]

Immunocytochemistry/Immunofluorescence: Lysine (K)-specific Demethylase 6B/KDM6B/JMJD3 Antibody [NBP2-82107] - Immunocytochemistry Validation of JMJD3 in K562 Cells. Immunocytochemical analysis of K562 cells using Lysine (K)-specific Demethylase 6B/KDM6B/JMJD3 antibody (NBP2-82107) at 2.5 ug/ml. Cells were fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.
Lysine (K)-specific Demethylase 6B/KDM6B/JMJD3 Antibody - BSA Free

Immunocytochemistry/ Immunofluorescence: Lysine (K)-specific Demethylase 6B/KDM6B/JMJD3 Antibody - BSA Free [NBP2-82107] -

Immunocytochemistry/ Immunofluorescence: Lysine (K)-specific Demethylase 6B/KDM6B/JMJD3 Antibody - BSA Free [NBP2-82107] - Validation of Lysine (K)-specific Demethylase 6B/KDM6B/JMJD3 in K562 Cells. Immunofluorescent analysis of 4% paraformaldehyde-fixed K562 cells labeling Lysine (K)-specific Demethylase 6B/KDM6B/JMJD3 with at 20 ug/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (red).

Applications for Lysine (K)-specific Demethylase 6B/KDM6B/JMJD3 Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry

2.5 ug/mL

Immunocytochemistry/ Immunofluorescence

2.5-20 ug/mL

Western Blot

0.25-4 ug/mL

Formulation, Preparation, and Storage

Purification

Peptide affinity purified

Formulation

PBS

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Lysine (K)-specific Demethylase 6B/KDM6B

JMJD3 is a histone demethylase that specifically demethylates trimethylated and dimethylated 'Lys-27' of histone H3, thereby playing a central role in histone code. JMJD3 is also important for the regulation of posterior development by regulating HOX gene expression. JMJD3 is involved in inflammatory response by participating in macrophage differentiation in case of inflammation by regulating gene expression and macrophage differentiation. It has also been shown to be essential to mammalian epidermal differentiation and is upregulated in prostate cancer.

JMJD3 antibodies are useful tools for epigenetics research and specifically for histone modification studies.

Alternate Names

JMJD3, KDM6B, Lysine (K)specific Demethylase 6B

Gene Symbol

KDM6B

Additional Lysine (K)-specific Demethylase 6B/KDM6B Products

Product Documents for Lysine (K)-specific Demethylase 6B/KDM6B/JMJD3 Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for Lysine (K)-specific Demethylase 6B/KDM6B/JMJD3 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

Epigenetics

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Protocols

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