Lysyl tRNA synthetase Antibody
Novus Biologicals | Catalog # NB100-1719
![Western Blot: Lysyl tRNA synthetase Antibody [NB100-1719]](https://resources.rndsystems.com/images/products/Lysyl-tRNA-synthetase-Antibody-Western-Blot-NB100-1719-img0002.jpg "Western Blot: Lysyl tRNA synthetase Antibody [NB100-1719]")
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Key Product Details
Species Reactivity
Human
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
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Product Specifications
Immunogen
The immunogen recognized by this antibody maps to a region between residue 550 and the C-terminus (residue 597) of human Lysyl-tRNA Synthetase using the numbering given in entry NP_005539.1 (GeneID 3735).
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for Lysyl tRNA synthetase Antibody
Western Blot: Lysyl tRNA synthetase Antibody [NB100-1719]
Western Blot: Lysyl tRNA synthetase Antibody [NB100-1719] - Samples: Whole cell lysate from HeLa (5, 15 and 50 mcg for WB; 1 mg for IP, 20% of IP loaded) and 293T (T; 50 mcg) cells. Affinity purified rabbit anti-Lysyl tRNA synthetase antibody NB100-1719 used for WB at 0.04 mcg/ml (A) and 1 mcg/ml (B) and used for IP at 3 mcg/mg lysate (B).![Immunocytochemistry/ Immunofluorescence: Lysyl tRNA synthetase Antibody [NB100-1719]](https://resources.rndsystems.com/images/products/Lysyl-tRNA-synthetase-Antibody-Immunocytochemistry-Immunofluorescence-NB100-1719-img0004.jpg "Immunocytochemistry/ Immunofluorescence: Lysyl tRNA synthetase Antibody [NB100-1719]")
Immunocytochemistry/ Immunofluorescence: Lysyl tRNA synthetase Antibody [NB100-1719]
Immunocytochemistry/Immunofluorescence: Lysyl tRNA synthetase Antibody [NB100-1719] - FFPE section of human breast carcinoma. Affinity purified rabbit anti-Lysyl tRNA synthetase used at a dilution of 1:100.![Immunohistochemistry: Lysyl tRNA synthetase Antibody [NB100-1719]](https://resources.rndsystems.com/images/products/Lysyl-tRNA-synthetase-Antibody-Immunohistochemistry-NB100-1719-img0007.jpg "Immunohistochemistry: Lysyl tRNA synthetase Antibody [NB100-1719]")
Immunohistochemistry: Lysyl tRNA synthetase Antibody [NB100-1719]
Immunohistochemistry: Lysyl tRNA synthetase Antibody [NB100-1719] - Sample: FFPE section of human ovarian carcinoma. Antibody: Affinity purified rabbit anti-KARS used at a dilution of 1:200 (1ug/ml). Detection: DABApplications for Lysyl tRNA synthetase Antibody
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
1:100 -1:500
Immunohistochemistry
1:10-1:500
Immunohistochemistry-Paraffin
1:10-1:500
Immunoprecipitation
1-4 ug/mg of lysate
Western Blot
1:2000-1:10000
Application Notes
Immunohistochemistry 1:100 to 1:500. Epitope retrieval with Tris-EDTA pH9.0 is recommended for FFPE tissue section
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
TBS and 0.1% BSA
Preservative
0.09% Sodium Azide
Concentration
0.2 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C. Do not freeze.
Background: Lysyl tRNA synthetase
Alternate Names
CMTRIB, EC 6.1.1, EC 6.1.1.6, KARS2, KIAA0070, KRS, lysine tRNA ligase, Lysine--tRNA ligase, lysRS, lysyl-tRNA synthetase
Entrez Gene IDs
3735 (Human)
Gene Symbol
KARS1
UniProt
Additional Lysyl tRNA synthetase Products
Product Documents for Lysyl tRNA synthetase Antibody
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for Lysyl tRNA synthetase Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Antigen Retrieval Protocol (PIER)
- Antigen Retrieval for Frozen Sections Protocol
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- Chromogenic IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Chromogenic Immunohistochemistry Staining of Frozen Tissue
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- Fluorescent IHC Staining of Frozen Tissue Protocol
- Graphic Protocol for Heat-induced Epitope Retrieval
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Graphic Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- ICC Cell Smear Protocol for Suspension Cells
- ICC Immunocytochemistry Protocol Videos
- ICC for Adherent Cells
- IHC Sample Preparation (Frozen sections vs Paraffin)
- Immunocytochemistry (ICC) Protocol
- Immunocytochemistry Troubleshooting
- Immunofluorescence of Organoids Embedded in Cultrex Basement Membrane Extract
- Immunofluorescent IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunohistochemistry Frozen Troubleshooting
- Immunohistochemistry Paraffin Troubleshooting
- Immunoprecipitation Protocol
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Protocol for Heat-Induced Epitope Retrieval (HIER)
- Protocol for Making a 4% Formaldehyde Solution in PBS
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Fluorescent ICC Staining of Cell Smears - Graphic
- Protocol for the Fluorescent ICC Staining of Cultured Cells on Coverslips - Graphic
- Protocol for the Preparation & Fixation of Cells on Coverslips
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections - Graphic
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections - Graphic
- Protocol for the Preparation and Fluorescent ICC Staining of Cells on Coverslips
- Protocol for the Preparation and Fluorescent ICC Staining of Non-adherent Cells
- Protocol for the Preparation and Fluorescent ICC Staining of Stem Cells on Coverslips
- Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- Protocol for the Preparation of a Cell Smear for Non-adherent Cell ICC - Graphic
- R&D Systems Quality Control Western Blot Protocol
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: Immunohistochemistry
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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