LYVE-1 Antibody - Azide and BSA Free

Novus Biologicals | Catalog # NB600-1008

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Cited:

Human, Mouse, Rat

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen, Immunohistochemistry Free-Floating, Western Blot, Flow Cytometry, CyTOF-ready

Cited:

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen, IF/IHC, IHC-F, IHC-Fr-Fl, IHC-Wh

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

Azide and BSA Free
View all LYVE-1 Primary Antibodies »

Product Specifications

Immunogen

Produced from sera of rabbits immunized with highly pure recombinant mouse soluble LYVE-1 produced in insect cells. The recombinant soluble LYVE-1 consists of amino acid 24 (Ala) to 228 (Gly) and is fused to a C-terminal His-tag (6xHis).

Reactivity Notes

Rat reactivity reported in (PMID: 24363089).

Localization

Plasma membrane

Marker

Lymphatic Vessel Marker

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

35 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for LYVE-1 Antibody - Azide and BSA Free

Immunohistochemistry-Paraffin: LYVE-1 Antibody [NB600-1008]

Immunohistochemistry-Paraffin: LYVE-1 Antibody [NB600-1008]

Immunohistochemistry-Paraffin: LYVE-1 Antibody [NB600-1008] - Positive staining for Lyve-1 on FFPE Mouse Liver, 20x magnification. Antibody diluted 1:800. Image from verified customer review.
Immunohistochemistry-Paraffin: LYVE-1 Antibody [NB600-1008]

Immunohistochemistry-Paraffin: LYVE-1 Antibody [NB600-1008]

Immunohistochemistry-Paraffin: LYVE-1 Antibody [NB600-1008] - Mouse kidney stained with LYVE-1 antibody. Image from verified customer review.
Western Blot: LYVE-1 Antibody [NB600-1008]

Western Blot: LYVE-1 Antibody [NB600-1008]

Western Blot: LYVE-1 Antibody [NB600-1008] - Analysis of anti-mouse LYVE-1. Sample was loaded in 15% SDS-polyacrylamide gel under reducing conditions.
Immunohistochemistry: LYVE-1 Antibody [NB600-1008]

Immunohistochemistry: LYVE-1 Antibody [NB600-1008]

LYVE-1 Antibody-Immunohistochemistry-NB600-1008-img0057.jpg
Immunohistochemistry-Paraffin: LYVE-1 Antibody [NB600-1008]

Immunohistochemistry-Paraffin: LYVE-1 Antibody [NB600-1008]

Immunohistochemistry-Paraffin: LYVE-1 Antibody [NB600-1008] - Mouse liver. Antibody at 1:800. Detection by Polymer-HRP. Image from verified customer review.
Immunohistochemistry: LYVE-1 Antibody [NB600-1008]

Immunohistochemistry: LYVE-1 Antibody [NB600-1008]

LYVE-1-Antibody-Immunohistochemistry-NB600-1008-img0052.jpg
LYVE-1 Antibody

Immunohistochemistry: LYVE-1 Antibody [NB600-1008] -

Hypoxia, blood vessel & proliferation staining. IHC staining in the Biopsy 23 & Biopsy 3 respectively for CA-9, EF5, CD31, Ki67 & LYVE1. 15× Magnification. Image collected & cropped by CiteAb from the following publication (http://www.mdpi.com/2072-6694/4/3/821), licensed under a CC-BY license. Not internally tested by Novus Biologicals.
LYVE-1 Antibody

Immunohistochemistry: LYVE-1 Antibody [NB600-1008] -

Immunohistochemistry: LYVE-1 Antibody [NB600-1008] - Zeb1-regulation of alkali-induced corneal NV in mice.a Reduction of Zeb1 mRNA in the heterozygous Zeb−/+ corneas compared to the homozygous Zeb1+/+ corneas. b Significant NV score & vessel size reduction in the Zeb1−/+ corneas as compared to their Zeb1+/+ siblings. c1 A representative stereoscopic image of the Zeb1−/+ & d1 Zeb1+/+ corneas & c2–d2 their whole flat-mount corneas immunostained with the endothelium marker CD31 & the lymphatic vessel marker LYVE-1. *p ≤ 0.05; **p ≤ 0.01. Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/32620870), licensed under a CC-BY license. Not internally tested by Novus Biologicals.
LYVE-1 Antibody

Immunohistochemistry: LYVE-1 Antibody [NB600-1008] -

Immunohistochemistry: LYVE-1 Antibody [NB600-1008] - Zeb1-regulation of alkali-induced corneal NV in mice.a Reduction of Zeb1 mRNA in the heterozygous Zeb−/+ corneas compared to the homozygous Zeb1+/+ corneas. b Significant NV score & vessel size reduction in the Zeb1−/+ corneas as compared to their Zeb1+/+ siblings. c1 A representative stereoscopic image of the Zeb1−/+ & d1 Zeb1+/+ corneas & c2–d2 their whole flat-mount corneas immunostained with the endothelium marker CD31 & the lymphatic vessel marker LYVE-1. *p ≤ 0.05; **p ≤ 0.01. Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/32620870), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Applications for LYVE-1 Antibody - Azide and BSA Free

Application
Recommended Usage

Flow Cytometry

3-10 ug/mL

Immunohistochemistry

1:10-1:500

Immunohistochemistry-Frozen

1:10-1:500

Immunohistochemistry-Paraffin

1:800

Western Blot

2-5 ug/mL
Application Notes
Use in IHC free floating reported in scientific literature (PMID: 30514806). Use in ICC/IF reported in secitific publication PMID: 32620870

Reviewed Applications

Read 3 reviews rated 4.7 using NB600-1008 in the following applications:

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A purified

Reconstitution

Centrifuge vial prior to opening. Reconstitute in sterile water to a concentration of 0.1-1.0 mg/ml. Please note the sample size of this product will be provided in reconstituted liquid form.

Formulation

PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

LYOPH mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: LYVE-1

LYVE-1 (Lymphatic Vessel Endothelial Receptor 1) is a receptor for the extracellular matrix mucopolysaccharide hyaluronan (HA) and is primarily expressed by lymphatic endothelial cells in addition to the sinusoidal endothelium of the liver and spleen (1-3). HA, also called hyaluronic acid, is a main component of the extracellular matrix and functions largely in cell adhesion, migration, and tissue remodeling (2). HA undergoes a turnover process that involves release from the tissues to the afferent lymph, degradation within the lymph nodes, and removal of fragments by the liver (2,3). LYVE-1, in addition to other lymphatic proteins including VEGFR3, Prox1, and podoplanin, is a common marker for differentiating between the blood and lymphatic systems (2,3). Furthermore, LYVE-1 is closely related to the leukocyte receptor CD44, having ~44% sequence similarity (1-3). Like CD44, the LYVE-1 protein contains an extracellular HA-binding link domain, with N- and C-terminal extensions, located at the end of a glycosylated juxtamembrane domain stalk region, followed by a transmembrane region, and a cytoplasmic tail (1-3). The key features of the HA-binding like molecule are the three disulfide bridges formed by six cysteine residues (1-3). LYVE-1 is synthesized as a protein of 322 amino acids in length with a theoretical molecular weight of 35 kDa, although due to O-glycosylation often appears in SDS-PAGE at a molecular weight ranging from ~60-70 kDa (2,4). The role of HA-binding is further elucidated by the identification of LYVE-1 as a docking receptor for dendritic cells and macrophages, binding their surface HA to control the entry and migration into lymph vessels (1).

LYVE-1 has been an important marker in studies of embryonic and tumor lymphangiogenesis, as many cancers are characterized by early metastasis to the lymph nodes (1-3, 5). One study of five different vascular tumors in infants used immunohistochemical analysis and found positive LYVE-1 expression in infantile hemangioma, tufted angioma, and kaposiform hemangioendothelioma (5). LYVE-1 along with other markers such as GLUT-1, CD31, CD34, Prox-1, and WT-1 can be used to help provide immunohistologic profiles of various tumors and, when used in conjunction with clinical and histopathologic approaches, may offer better overall diagnosis and disease treatment (5).

References

1. Jackson D. G. (2019). Hyaluronan in the lymphatics: The key role of the hyaluronan receptor LYVE-1 in leucocyte trafficking. Matrix Biology : Journal of the International Society for Matrix Biology. https://doi.org/10.1016/j.matbio.2018.02.001

2. Jackson D. G. (2004). Biology of the lymphatic marker LYVE-1 and applications in research into lymphatic trafficking and lymphangiogenesis. APMIS : acta pathologica, microbiologica, et immunologica Scandinavica. https://doi.org/10.1111/j.1600-0463.2004.apm11207-0811.x

3. Jackson D. G. (2003). The lymphatics revisited: new perspectives from the hyaluronan receptor LYVE-1. Trends in Cardiovascular Medicine. https://doi.org/10.1016/s1050-1738(02)00189-5

4. Unitprot (Q9Y5Y7)

5. Johnson, E. F., Davis, D. M., Tollefson, M. M., Fritchie, K., & Gibson, L. E. (2018). Vascular Tumors in Infants: Case Report and Review of Clinical, Histopathologic, and Immunohistochemical Characteristics of Infantile Hemangioma, Pyogenic Granuloma, Noninvoluting Congenital Hemangioma, Tufted Angioma, and Kaposiform Hemangioendothelioma. The American Journal of Dermatopathology. https://doi.org/10.1097/DAD.0000000000000983

Long Name

Lymphatic Vessel Endothelial Hyaluronan Receptor 1

Alternate Names

LYVE1, XLKD1

Entrez Gene IDs

114332 (Mouse)

Gene Symbol

LYVE1

UniProt

Q8BHC0

Additional LYVE-1 Products

Product Documents for LYVE-1 Antibody - Azide and BSA Free

Certificate of Analysis

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Product Specific Notices for LYVE-1 Antibody - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for LYVE-1 Antibody - Azide and BSA Free

Customer Reviews for LYVE-1 Antibody - Azide and BSA Free (3)

4.7 out of 5
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Customer Images

Showing  1 - 3 of 3 reviews Showing All
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  • LYVE-1 Antibody
    Name: Anonymous
    Application: Immunohistochemistry-Paraffin
    Sample Tested: mouse liver
    Species: Mouse
    Verified Customer | Posted 06/07/2019
    Mouse Liver, 20x magnification
    Antibody working dilution 1:800; Citrate pH 6.0. Detection kit Polymer-HRP.
    LYVE-1 Antibody - Azide and BSA Free NB600-1008
  • LYVE-1 Antibody
    Name: Anonymous
    Application: Immunohistochemistry
    Sample Tested: Formalin fixed paraffin embedded mouse liver
    Species: Mouse
    Verified Customer | Posted 06/29/2017
    IHC positive staining for Lyve-1 NB600-1008 on FFPE Mouse Liver, 20x
    WD [1:800], HIER with Citrate Buffer pH 6.0 10mM; secondary ab RTU Polymer-HRP
    LYVE-1 Antibody - Azide and BSA Free NB600-1008
  • Name: Sarah Bowhay
    Application: Immunohistochemistry-Paraffin
    Sample Tested: Mouse kidney
    Species: Mouse
    Verified Customer | Posted 03/15/2016
    LYVE-1 Antibody staining lymph vessels in the kidney
    LYVE-1 Antibody - Azide and BSA Free NB600-1008

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