MafA Antibody (BLR067G) - BSA Free

Novus Biologicals | Catalog # NBP2-78707

Recombinant Monoclonal Antibody
Novus Biologicals
100% Guarantee

Key Product Details

Species Reactivity

Human, Mouse

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # BLR067G

Format

BSA Free
View all MafA Primary Antibodies »

Product Specifications

Immunogen

Immunogen was a peptide representing a region between residue 125 and 175 of human MAF bZIP transcription factor A using the numbering given in entry NP_963883.2 (Gene ID 389692).

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for MafA Antibody (BLR067G) - BSA Free

Western Blot: MafA Antibody (BLR067G) [NBP2-78707]

Western Blot: MafA Antibody (BLR067G) [NBP2-78707]

Western Blot: MafA Antibody (BLR067G) [NBP2-78707] - Detection of human and mouse MafA by WB of HEK293T transfected with myc tagged human or mouse MafA, MafB or c-Maf using this antibody, rabbit anti-MafB recombinant monoclonal [BLR046F], and rabbit anti-c-Maf recombinant monoclonal [BLR045F]. Secondary: HRP-conjugated goat anti-rabbit IgG (NB100-2332 ). Lower panel: Rabbit anti-Myc.
Immunocytochemistry/ Immunofluorescence: MafA Antibody (BLR067G) [NBP2-78707]

Immunocytochemistry/ Immunofluorescence: MafA Antibody (BLR067G) [NBP2-78707]

Immunocytochemistry/Immunofluorescence: MafA Antibody (BLR067G) [NBP2-78707] - Rabbit anti-MafA recombinant monoclonal antibody [BLR067G]. Secondary: HRP-conjugated goat anti-rabbit IgG. Substrate: DAB.
Immunohistochemistry-Paraffin: MafA Antibody (BLR067G) [NBP2-78707]

Immunohistochemistry-Paraffin: MafA Antibody (BLR067G) [NBP2-78707]

Immunohistochemistry-Paraffin: MafA Antibody (BLR067G) [NBP2-78707] - Rabbit anti-MafA recombinant monoclonal antibody [BLR067G] Secondary: HRP-conjugated goat anti-rabbit IgG. Substrate: DAB.
Western Blot: MafA Antibody (BLR067G) [NBP2-78707]

Western Blot: MafA Antibody (BLR067G) [NBP2-78707]

Western Blot: MafA Antibody (BLR067G) [NBP2-78707] - Detection of mouse MafA by WB of Beta-TC-6, AlphaTC1 Clone 9, and NIH 3T3 lysate using this antibody. Secondary: HRP-conjugated goat anti-rabbit IgG (NB100-2332 ).
Immunohistochemistry-Paraffin: MafA Antibody (BLR067G) [NBP2-78707]

Immunohistochemistry-Paraffin: MafA Antibody (BLR067G) [NBP2-78707]

Immunohistochemistry-Paraffin: MafA Antibody (BLR067G) [NBP2-78707] - Detection of human MafA (red) in FFPE pancreatic carcinoma by IHC-IF using this antibody. Secondary: HRP-conjugated goat anti-rabbit IgG (NB100-2332). Substrate: Opal™. Counterstain: DAPI (blue).

Applications for MafA Antibody (BLR067G) - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100 - 1:500

Immunohistochemistry

1:100 - 1:500

Western Blot

1:1000
Application Notes
Centrifuge tube to remove product from lid. Optimal working dilutions should be determined experimentally by the investigator. Prepare working dilution immediately before use. IHC and ICC/IF: Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections. Zinc-fixative (JB fix) is recommended to enhance staining.

Formulation, Preparation, and Storage

Purification

Protein A or G purified from cell culture supernatant

Formulation

Borate Buffered Saline (BBS) pH 8.2.

Format

BSA Free

Preservative

0.09% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 2 - 8 C / 1 year from date of receipt

Background: MafA

Insulin gene expression is regulated by several islet-enriched transcription factors. However, MafA is the only beta cell-specific activator. MafA selectively induces endogenous insulin transcription in non-beta cells. MafA was also first detected in the insulin-producing cells formed during the second and predominant phase of beta cell differentiation, and absent in the few insulin-positive cells found in Nkx6.1(-/-) pancreata, which lack the majority of second-phase beta cells. These results demonstrate that MafA is a potent insulin activator that is likely to function downstream of Nkx6.1 during islet insulin-producing cell development.

Alternate Names

hMafA, Pancreatic beta-cell-specific transcriptional activator, RIPE3b1, transcription factor MafA, Transcription factor RIPE3b1, V-maf musculoaponeurotic fibrosarcoma oncogene homolog A, v-maf musculoaponeurotic fibrosarcoma oncogene homolog A (avian)

Entrez Gene IDs

NP_963883.2 (Human)

Gene Symbol

MAFA

Additional MafA Products

Product Documents for MafA Antibody (BLR067G) - BSA Free

Certificate of Analysis

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Product Specific Notices for MafA Antibody (BLR067G) - BSA Free

This Product sold under License from Bethyl Laboratories, Inc.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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