Key Product Details

Species Reactivity

Validated:

Human, Mouse

Cited:

Human, Mouse

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen, Western Blot, Immunocytochemistry/ Immunofluorescence, Chromatin Immunoprecipitation (ChIP), Gel Super Shift Assays

Cited:

Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen, Western Blot, Immunocytochemistry/ Immunofluorescence, Chemotaxis

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all MafA Primary Antibodies »

Product Specifications

Immunogen

The immunogen recognized by this antibody maps to a region between residue 300 and the C-terminus (residue 359) of mouse v-maf musculoaponeurotic fibrosarcoma oncogene homolog A using the numbering given in entry NP_919331.1 (GeneID 378435).

Reactivity Notes

Human reactivity reported in the scientific literature (PMID: 22665223).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for MafA Antibody - BSA Free

Western Blot: MafA Antibody [NB400-137]

Western Blot: MafA Antibody [NB400-137]

Western Blot: MafA Antibody [NB400-137] - Nuclear extract (6 ug) from HeLa cells transfected with MafA, MafB or cMaf expression constructs. Affinity purified anti-MafA antibody (NB400-137), anti-MafB antibody (NB600-266) or anti-c-Maf antibody (NB600-267). Each antibody was used at 0.5 ug/ml.
Immunohistochemistry-Paraffin: MafA Antibody [NB400-137]

Immunohistochemistry-Paraffin: MafA Antibody [NB400-137]

Immunohistochemistry-Paraffin: MafA Antibody [NB400-137] - Immunohistochemical staining of MafA (blue) and insulin (green) in mouse pancreas. Image from verified customer review.
Western Blot: MafA Antibody [NB400-137]

Western Blot: MafA Antibody [NB400-137]

Western Blot: MafA Antibody [NB400-137] - Binding of MafA to the enhancer region of the endogenous insulin gene. Samples: Immunoprecipitated cross-linked DNA from betaTC-3 cells was analyzed by PCR. As controls, reactions were run with no DNA, with input chromatin, with DNA obtained after precipitation with rabbit IgG or without antibody (lanes 1 through 5, respectively). Antibody: Affinity purified rabbit anti-MafA antibody (NB 400-137).
Gel Super Shift Assays: MafA Antibody [NB400-137]

Gel Super Shift Assays: MafA Antibody [NB400-137]

Gel Super Shift Assays: MafA Antibody [NB400-137] - Electrophoretic Mobility Shift of MafA, MafB and c-Maf. Samples: Nuclear extract (6 ug) from HeLa cells transfected with MafA, MafB or c-Maf expression constructs. Antibodies: Affinity purified anti-MafA antibody NB400-137, anti-MafB antibody NB600-266 or anti-c-Maf antibody NB600-267.

Applications for MafA Antibody - BSA Free

Application
Recommended Usage

Gel Super Shift Assays

1 to 5 ug/20 ul reaction

Immunocytochemistry/ Immunofluorescence

1:10-1:500

Immunohistochemistry

1:10-1:500

Immunohistochemistry-Frozen

1:10-1:500

Immunohistochemistry-Paraffin

1:1000 to 1:3000

Western Blot

1:1000-1:10000
Application Notes
A band ~36-40 kDa is seen in WB analysis. Use in IHC-P was reported in the scientific literature (PMID: 22665223). In some cases, the antibody may be diluted further than indicated. Optimal working dilutions should be determined experimentally by the investigator. Prepare working dilution immediately before use. Use in ICC/IF and in IHC-Frozen reported in scientific literature (PMID 23967072). Chip reported in scientific literature (PMID: 26314560).

Reviewed Applications

Read 1 review rated 5 using NB400-137 in the following applications:

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris-Citrate/Phosphate (pH 7.0 - 8.0)

Format

BSA Free

Preservative

0.09% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: MafA

Insulin gene expression is regulated by several islet-enriched transcription factors. However, MafA is the only beta cell-specific activator. MafA selectively induces endogenous insulin transcription in non-beta cells. MafA was also first detected in the insulin-producing cells formed during the second and predominant phase of beta cell differentiation, and absent in the few insulin-positive cells found in Nkx6.1(-/-) pancreata, which lack the majority of second-phase beta cells. These results demonstrate that MafA is a potent insulin activator that is likely to function downstream of Nkx6.1 during islet insulin-producing cell development.

Alternate Names

hMafA, Pancreatic beta-cell-specific transcriptional activator, RIPE3b1, transcription factor MafA, Transcription factor RIPE3b1, V-maf musculoaponeurotic fibrosarcoma oncogene homolog A, v-maf musculoaponeurotic fibrosarcoma oncogene homolog A (avian)

Entrez Gene IDs

378435 (Mouse)

Gene Symbol

MAFA

UniProt

Q8CF90 (Mouse)

Additional MafA Products

Product Documents for MafA Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for MafA Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for MafA Antibody - BSA Free

Customer Reviews for MafA Antibody - BSA Free (1)

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Customer Images

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  • Name: Yves Heremans
    Application: Immunohistochemistry-Paraffin
    Sample Tested: Mouse Pancreas
    Species: Mouse
    Verified Customer | Posted 09/21/2015
    MafA-insulin
    MafA Antibody - BSA Free NB400-137

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