MAGEA1 Antibody - BSA Free

Novus Biologicals | Catalog # NBP3-38158

Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, ELISA, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all MAGEA1 Primary Antibodies »

Product Specifications

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 1-309 of human MAGEA1 (NP_004979.3).

Sequence:
MSLEQRSLHCKPEEALEAQQEALGLVCVQAATSSSSPLVLGTLEEVPTAGSTDPPQSPQGASAFPTTINFTRQRQPSEGSSSREEEGPSTSCILESLFRAVITKKVADLVGFLLLKYRAREPVTKAEMLESVIKNYKHCFPEIFGKASESLQLVFGIDVKEADPTGHSYVLVTCLGLSYDGLLGDNQIMPKTGFLIIVLVMIAMEGGHAPEEEIWEELSVMEVYDGREHSAYGEPRKLLTQDLVQEKYLEYRQVPDSDPARYEFLWGPRALAETSYVKVLEYVIKVSARVRFFFPSLREAALREEEEGV

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

34 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for MAGEA1 Antibody - BSA Free

MAGEA1 Antibody

Immunohistochemistry: MAGEA1 Antibody [NBP3-38158] -

Immunohistochemistry: MAGEA1 Antibody [NBP3-38158] - Immunohistochemistry analysis of paraffin-embedded Human breast cancer using MAGEA1 Rabbit pAb at dilution of 1:200 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.
MAGEA1 Antibody

Immunohistochemistry: MAGEA1 Antibody [NBP3-38158] -

Immunohistochemistry: MAGEA1 Antibody [NBP3-38158] - Immunohistochemistry analysis of paraffin-embedded Human stomach cancer using MAGEA1 Rabbit pAb at dilution of 1:200 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.
MAGEA1 Antibody

Immunohistochemistry: MAGEA1 Antibody [NBP3-38158] -

Immunohistochemistry: MAGEA1 Antibody [NBP3-38158] - Immunohistochemistry analysis of paraffin-embedded Human liver cancer using MAGEA1 Rabbit pAb at dilution of 1:200 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.
MAGEA1 Antibody

Immunocytochemistry/ Immunofluorescence: MAGEA1 Antibody [NBP3-38158] -

Immunocytochemistry/ Immunofluorescence: MAGEA1 Antibody [NBP3-38158] - Immunofluorescence analysis of HeLa cells using MAGEA1 Rabbit pAb. Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
MAGEA1 Antibody

Western Blot: MAGEA1 Antibody [NBP3-38158] -

Western Blot: MAGEA1 Antibody [NBP3-38158] - Western blot analysis of lysates from 22Rv1 cells, using MAGEA1 Rabbit pAb at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 30s.

Applications for MAGEA1 Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:50 - 1:100

Immunohistochemistry-Paraffin

1:50 - 1:200

Western Blot

1:500 - 1:2000

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.3), 50% glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: MAGEA1

The MAGE 1 gene is a member of the melanoma antigen encoding gene family. These genes encode for HLA-restricted tumor associated rejection antigens recognized by cytotoxic T lymphocytes. Some of these target antigens may be potentially useful for cancer specific immunotherapy. The expression of MAGE genes has been reported not only in melanoma but also in various other malignant tumors such as hepatocellular carcinoma and germ cell tumors.

Long Name

Melanoma-Associated Antigen 1

Alternate Names

CT1.1, MAGE-A1, MAGE1, MGC9326, MZ2-E

Gene Symbol

MAGEA1

Additional MAGEA1 Products

Product Documents for MAGEA1 Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for MAGEA1 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

Cancer Stem Cell Markers

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Protocols

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FAQs

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