MAGP-2/MFAP5 Antibody - BSA Free

Novus Biologicals | Catalog # NBP1-82803

Novus Biologicals
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Key Product Details

Validated by

Orthogonal Validation

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Cited:

Western Blot, IF/IHC

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all MAGP-2/MFAP5 Primary Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: GVNSQRGDDVTQATPETFTEDPNLVNDPATDETVLAVLADIAPSTDDLASLSEKNTTAECWDEKFTCTRLYSVHRPVKQCIHQLCFTSLRRMYIVNKEICSRLVCKEHEAMKDELCRQMAGLPPRRLRRSNYFRLPPCENV

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Mouse (81%), Rat (83%).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for MAGP-2/MFAP5 Antibody - BSA Free

Immunohistochemistry-Paraffin: MAGP-2/MFAP5 Antibody [NBP1-82803]

Immunohistochemistry-Paraffin: MAGP-2/MFAP5 Antibody [NBP1-82803]

Immunohistochemistry-Paraffin: MAGP-2/MFAP5 Antibody [NBP1-82803] - Staining in human smooth muscle and cerebral cortex tissues using anti-MFAP5 antibody. Corresponding MFAP5 RNA-seq data are presented for the same tissues.
Western Blot: MAGP-2/MFAP5 Antibody [NBP1-82803]

Western Blot: MAGP-2/MFAP5 Antibody [NBP1-82803]

Western Blot: MAGP-2/MFAP5 Antibody [NBP1-82803] - Analysis in human cell line BEWO.
Immunohistochemistry-Paraffin: MAGP-2/MFAP5 Antibody [NBP1-82803]

Immunohistochemistry-Paraffin: MAGP-2/MFAP5 Antibody [NBP1-82803]

Immunohistochemistry-Paraffin: MAGP-2/MFAP5 Antibody [NBP1-82803] - Staining of human cerebral cortex shows low expression as expected.
Immunohistochemistry-Paraffin: MAGP-2/MFAP5 Antibody [NBP1-82803]

Immunohistochemistry-Paraffin: MAGP-2/MFAP5 Antibody [NBP1-82803]

Immunohistochemistry-Paraffin: MAGP-2/MFAP5 Antibody [NBP1-82803] - Staining of human smooth muscle shows high expression.
MAGP-2/MFAP5 Antibody

Immunohistochemistry-Paraffin: MAGP-2/MFAP5 Antibody [NBP1-82803] -

Immunohistochemistry-Paraffin: MAGP-2/MFAP5 Antibody [NBP1-82803] - Staining of human rectum shows strong membranous positivity in cells in lamina propria.
MAGP-2/MFAP5 Antibody

Immunohistochemistry-Paraffin: MAGP-2/MFAP5 Antibody [NBP1-82803] -

Immunohistochemistry-Paraffin: MAGP-2/MFAP5 Antibody [NBP1-82803] - Staining of human testis shows strong membranous positivity in cells in lamina propria.

Applications for MAGP-2/MFAP5 Antibody - BSA Free

Application
Recommended Usage

Immunohistochemistry

1:500 - 1:1000

Immunohistochemistry-Paraffin

1:500 - 1:1000

Western Blot

0.04-0.4 ug/ml
Application Notes
IHC-Paraffin, HIER pH 6 retrieval is recommended.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: MAGP-2/MFAP5

MFAP5, also known as Microfibrillar-associated protein 5, is a 173 amino acid protein that is 20 kDa, extracellular matrix secreted, and it is a component of the elastin-associated microfibrils. Current research is being performed on this protein involvement in marfan syndrome, scleroderma and skin fibrosis. The protein is involved in molecules associated with elastic fibres, extracellular matrix organization, elastic fibre formation, tropoelastin associates with microfibrils, elastin cross-linking by lysyl oxidase, and notch signaling pathways where it interacts with FBN2, JAG1, JAG2, MEGF6, VWF, FBLN1, FBN1, GYG1, DLL1, MFAP2, NOTCH1, ENAM, FBN3, and DLL4 proteins.

Long Name

Microfibril-associated Glycoprotein 2

Alternate Names

MAGP2, MFAP5, MP25

Gene Symbol

MFAP5

Additional MAGP-2/MFAP5 Products

Product Documents for MAGP-2/MFAP5 Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for MAGP-2/MFAP5 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for MAGP-2/MFAP5 Antibody - BSA Free

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Protocols

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FAQs

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Associated Pathways

Notch Signaling Pathways Notch Signaling Pathway Thumbnail