Malondialdehyde Antibody (6H6) - BSA Free

Novus Biologicals | Catalog # NBP2-59366

Novus Biologicals

Key Product Details

Validated by

Biological Validation

Species Reactivity

Non-species specific

Applications

Validated:

Western Blot, ELISA, Flow Cytometry, Immunocytochemistry/ Immunofluorescence

Cited:

Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 6H6

Format

BSA Free
View all Malondialdehyde Primary Antibodies »

Product Specifications

Immunogen

Synthetic Malondialdehyde modified Keyhole Limpet Hemocyanin (KLH).

Specificity

Specific for Malondialdehyde conjugated proteins. Does not detect free Malondialdehyde. Does not cross-react with Acrolein, Crotonaldehyde, Hexanoyl Lysine, 4-Hydroxy-2-hexenal, 4-Hydroxy nonenal, or Methylglyoxal modified proteins.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for Malondialdehyde Antibody (6H6) - BSA Free

Western Blot: Malondialdehyde Antibody (6H6) [NBP2-59366]

Western Blot: Malondialdehyde Antibody (6H6) [NBP2-59366]

Western Blot: Malondialdehyde Antibody (6H6) [NBP2-59366] - Western Blot analysis of Malondialdehyde-BSA Conjugate showing detection of 67 kDa Malondialdehyde protein using Mouse Anti-Malondialdehyde Monoclonal Antibody, Clone 6H6 (NBP2-59366). Lane 1: Molecular Weight Ladder (MW). Lane 2: Malondialdehyde-BSA (0.5 ug). Lane 3: Malondialdehyde-BSA (2.0 ug). Lane 4: BSA (0.5 ug). Lane 5: BSA (2.0 ug). Block: 5% Skim Milk in TBST. Primary Antibody: Mouse Anti-Malondialdehyde Monoclonal Antibody (NBP2-59366) at 1:1000 for 2 hours at RT. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:2000 for 60 min at RT. Color Development: ECL solution for 5 min in RT. Predicted/Observed Size: 67 kDa.
Immunocytochemistry/ Immunofluorescence: Malondialdehyde Antibody (6H6) [NBP2-59366]

Immunocytochemistry/ Immunofluorescence: Malondialdehyde Antibody (6H6) [NBP2-59366]

Immunocytochemistry/Immunofluorescence: Malondialdehyde Antibody (6H6) [NBP2-59366] - Tissue: Embryonic kidney cells (HEK293). Species: Human. Fixation: 5% Formaldehyde for 5 min. Primary Antibody: Mouse Anti-Malondialdehyde Monoclonal Antibody at 1:50 for 30-60 min at RT. Secondary Antibody: Goat Anti-Mouse Alexa Fluor 488 at 1:1500 for 30-60 min at RT. Counterstain: Phalloidin Alexa Fluor 633 F-Actin stain; DAPI (blue) nuclear stain at 1:250, 1:50000 for 30-60 min at RT. Magnification: 20X (2X Zoom). (A,C,E,G) - Untreated. (B,D,F,H) - Cells cultured overnight with 50 uM H2O2. (A,B) DAPI (blue) nuclear stain. (C,D) Phalloidin Alex Fluor 633 F-Actin stain. (E,F) Malondialdehyde Antibody. (G,H) Composite. Courtesy of: Dr. Robert Burke, University of Victoria.
Flow Cytometry: Malondialdehyde Antibody (6H6) [NBP2-59366]

Flow Cytometry: Malondialdehyde Antibody (6H6) [NBP2-59366]

Flow Cytometry: Malondialdehyde Antibody (6H6) [NBP2-59366] - Flow Cytometry analysis using Mouse Anti-Malondialdehyde Monoclonal Antibody, Clone 6H6 (NBP2-59366). Tissue: Neuroblastoma cells (SH-SY5Y). Species: Human. Fixation: 90% Methanol. Primary Antibody: Mouse Anti-Malondialdehyde Monoclonal Antibody (NBP2-59366) at 1:50 for 30 min on ice. Secondary Antibody: Goat Anti-Mouse: PE at 1:100 for 20 min at RT. Isotype Control: Non Specific IgG. Cells were subject to oxidative stress by treating with 250 M H2O2 for 24 hours.
Malondialdehyde Antibody (6H6) - BSA Free

Western Blot: Malondialdehyde Antibody (6H6) - BSA Free [NBP2-59366] -

AK046375 alleviates H2O2-induced oxidative stress in primary neurons and astrocytes. (1) Western blotting images of MT2, MDA, CAT, and SOD2 in neurons (a) and astrocytes (d) for each group, and the content of GSH, GSSG, and GSH/GSSG in neurons (b) and astrocytes (e) with quantification. (2) Fluorescence of MitoSOXred in neurons (c) and astrocytes (f) and quantification (scale bar = 50 μm, 400x for neurons; scale bar = 50 μm, 200x for astrocytes) (n = 6/group, mean +/- SD, ∗P < 0.05 vs. the overexpression vector+H2O2 group, &P < 0.05 vs. the knockdown vector+H2O2 group by one-way ANOVA). Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/35222805), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Applications for Malondialdehyde Antibody (6H6) - BSA Free

Application
Recommended Usage

ELISA

1:1000

Flow Cytometry

1:50

Immunocytochemistry/ Immunofluorescence

1:50

Western Blot

1:1000

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS (pH 7.4), 50% Glycerol

Format

BSA Free

Preservative

0.09% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C.

Background: Malondialdehyde

Alternate Names

3-Propanedial;Malonaldehyde;Malondialdehyde;MDA

Additional Malondialdehyde Products

Product Documents for Malondialdehyde Antibody (6H6) - BSA Free

Certificate of Analysis

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Product Specific Notices for Malondialdehyde Antibody (6H6) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for Malondialdehyde Antibody (6H6) - BSA Free

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Protocols

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