Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Predicted:

Bovine (92%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, ELISA, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all MANF Primary Antibodies »

Product Specifications

Immunogen

Antibody was raised against a 12 amino acid synthetic peptide from near the amino terminus of human MANF. The immunogen is located within the first 50 amino acids of MANF. Amino Acid Squence: GGG LRPGDCEVCISY

Specificity

This antibody does not cross-react with CDNF.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

26 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

Novus Biologicals Rabbit MANF Antibody - BSA Free (NBP1-76837) is a polyclonal antibody validated for use in IHC, WB, ELISA and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.

Scientific Data Images for MANF Antibody - BSA Free

Western Blot: MANF AntibodyBSA Free [NBP1-76837]

Western Blot: MANF AntibodyBSA Free [NBP1-76837]

Western Blot: MANF Antibody [NBP1-76837] - Western Blot Validation with Recombinant Protein. Loading: 30 ng of human MANF recombinant protein per lane. Antibodies: MANF NBP1-76837 (Lane 1: 0.125 ug/mL, Lane 2: 0.25 ug/mL and Lane 3: 0.5 ug/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution. Observed at around 55kDa.
Western Blot: MANF AntibodyBSA Free [NBP1-76837]

Western Blot: MANF AntibodyBSA Free [NBP1-76837]

Western Blot: MANF Antibody [NBP1-76837] - Rat brain tissue lysate with ARMET antibody at (A) 1 and (B) 2 ug/mL.
Western Blot: MANF AntibodyBSA Free [NBP1-76837]

Western Blot: MANF AntibodyBSA Free [NBP1-76837]

Western Blot: MANF Antibody [NBP1-76837] - Western Blot Validation in Human Cell Lines. Loading: 15 ug of lysates per lane. Antibodies: MANF NBP1-76837, (2 ug/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
MANF Antibody - BSA Free

Immunohistochemistry: MANF Antibody - BSA Free [NBP1-76837] -

Immunohistochemistry: MANF Antibody - BSA Free [NBP1-76837] - Validation of MANF in Human Brain Tissue.Immunohistochemical analysis of paraffin-embedded human brain tissue using anti-MANF antibody at 2.5 ug/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.
MANF Antibody - BSA Free

Immunocytochemistry/ Immunofluorescence: MANF Antibody - BSA Free [NBP1-76837] -

Immunocytochemistry/ Immunofluorescence: MANF Antibody - BSA Free [NBP1-76837] - Validation of MANF in Human Brain Tissue. Immunofluorescent analysis of 4% paraformaldehyde-fixed human brain tissue labeling MANF with at 20 ug/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (red).

Applications for MANF Antibody - BSA Free

Application
Recommended Usage

ELISA

1:100-1:2000

Immunocytochemistry/ Immunofluorescence

20 ug/ml

Immunohistochemistry

2.5 ug/mL

Western Blot

0.125-2 ug/mL

Formulation, Preparation, and Storage

Purification

Peptide affinity purified

Formulation

PBS

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: MANF

MANF, also known as ARMET, was initially identified as a protein containing an arginine-rich region that was highly mutated in a variety of tumors. More recently it was identified as a mesencephalic astrocyte-derived neurotrophic factor with selectivity for dopaminergic neurons, similar to glial cell line-derived neurotrophic factor (GDNF) and CDNF. In rat brain slices, MANF enhanced nigral gamma-aminobutyric acid release. Like GDNF and CDNF, MANF has selective neuroprotective activity for dopaminergic neurons suggesting that it may be indicated for the treatment of Parkinson's disease. Expression of MANF has also been shown to be induced during ER stress, suggesting that it may play a role in protein quality control during ER stress. This antibody does not cross-react with CDNF.

Long Name

Mesencephalic Astrocyte-derived Neurotrphic Factor

Alternate Names

ARMET, ARP

Gene Symbol

MANF

UniProt

P55145 (Human)

Additional MANF Products

Product Documents for MANF Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for MANF Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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