MAP1 Antibody (4A1) - Azide and BSA Free

Western Blot: MAP1 Antibody (4A1) - Azide and BSA Free [H00064112-M02] -

Comparison of downregulation of apoptosis/MOAP1 by siRNA of MOAP1 and hcmv-miR-UL70-3p: (a) Through DAPI imaging, images (63×) showing the nuclear morphology. (b) Measurement of Caspase 3/7 activity: The Caspase 3/7 activities were measured through Caspase Glo 3/7 assay in four different groups of cells, as mentioned above, these were recorded as raw luminescence units (RLU) (+/-SEM; ****p < 0.0001). (c) MOAP1 mRNA downregulation: The relative expression levels of MOAP1 mRNA after the transfection of either hcmv-miR-UL70-3p and siRNA of MOAP1 were measured through qRT-PCR. Results were expressed as the fold change (2− delta delta Ct) (+/- SEM; **, p < 0.01; ****, p < 0.0001). (d) MOAP1 protein downregulation: The MOAP1 protein downregulation after transfection with either siRNA of MOAP1 and hcmv-miR-UL70-3p were analyzed through Western blot. The relative MOAP1 protein quantification was performed through ImageJ software after normalizing with beta -actin. Experiments were performed in triplicates (Supplementary Figure S2b), and the data from three different experiments were used for statistical analysis (+/-SEM; *, p < 0.05; **, p < 0.01, ns = non-significant). Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/35008453), licensed under a CC-BY license. Not internally tested by Novus Biologicals.