MAP1B Antibody - BSA Free

Novus Biologicals | Catalog # NBP1-81261

Novus Biologicals
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Key Product Details

Validated by

Knockout/Knockdown, Orthogonal Validation

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence, Knockdown Validated

Cited:

IF/IHC

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all MAP1B Primary Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: EVVEEHCASPEDKTLEVVSPSQSVTGSAGHTPYYQSPTDEKSSHLPTEVIEKPPAVPVSFEFSDAKDENERASVSPMDEPVPDSESPIEKVLSPLRSPPLIGSESAYESFLSADDKASGRGAESPFEEKSGKQGSPDQVSPVSEMTST

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Mouse (85%), Rat (86%).

Marker

Neuronal Marker

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Description

Novus Biologicals Rabbit MAP1B Antibody - BSA Free (NBP1-81261) is a polyclonal antibody validated for use in IHC, WB and ICC/IF. Anti-MAP1B Antibody: Cited in 2 publications. All Novus Biologicals antibodies are covered by our 100% guarantee.

Scientific Data Images for MAP1B Antibody - BSA Free

Immunohistochemistry-Paraffin: MAP1B Antibody [NBP1-81261]

Immunohistochemistry-Paraffin: MAP1B Antibody [NBP1-81261]

Immunohistochemistry-Paraffin: MAP1B Antibody [NBP1-81261] - Analysis in human cerebral cortex and skeletal muscle tissues using NBP1-81261 antibody. Corresponding MAP1B RNA-seq data are presented for the same tissues.
Western Blot: MAP1B Antibody [NBP1-81261]

Western Blot: MAP1B Antibody [NBP1-81261]

Western Blot: MAP1B Antibody [NBP1-81261] - Analysis in U-251MG cells transfected with control siRNA, target specific siRNA probe #1 and #2, using Anti-MAP1B antibody. Remaining relative intensity is presented. Loading control: Anti-GAPDH.
Immunocytochemistry/ Immunofluorescence: MAP1B Antibody [NBP1-81261]

Immunocytochemistry/ Immunofluorescence: MAP1B Antibody [NBP1-81261]

Immunocytochemistry/Immunofluorescence: MAP1B Antibody [NBP1-81261] - Immunofluorescent staining of human cell line U-2 OS shows localization to cytosol.
Immunohistochemistry-Paraffin: MAP1B Antibody [NBP1-81261]

Immunohistochemistry-Paraffin: MAP1B Antibody [NBP1-81261]

Immunohistochemistry-Paraffin: MAP1B Antibody [NBP1-81261] - Staining of human cerebellum shows moderate to strong membranous positivity in cells in granular layer.
Immunohistochemistry-Paraffin: MAP1B Antibody [NBP1-81261]

Immunohistochemistry-Paraffin: MAP1B Antibody [NBP1-81261]

Immunohistochemistry-Paraffin: MAP1B Antibody [NBP1-81261] - Staining of human cerebral cortex shows moderate to strong cytoplasmic positivity in neurons.
Immunohistochemistry-Paraffin: MAP1B Antibody [NBP1-81261]

Immunohistochemistry-Paraffin: MAP1B Antibody [NBP1-81261]

Immunohistochemistry-Paraffin: MAP1B Antibody [NBP1-81261] - Staining of human skeletal muscle shows low positivity in myocytes as expected.
Immunohistochemistry-Paraffin: MAP1B Antibody [NBP1-81261]

Immunohistochemistry-Paraffin: MAP1B Antibody [NBP1-81261]

Immunohistochemistry-Paraffin: MAP1B Antibody [NBP1-81261] - Staining of human testis shows moderate membranous positivity in cells in seminiferous ducts.

Applications for MAP1B Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:1000 - 1:2500

Immunohistochemistry-Paraffin

1:1000 - 1:2500

Western Blot

0.04 - 0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: MAP1B

The MAP1B gene encodes a protein that belongs to the microtubule-associated protein family. The proteins of this family are thought to be involved in microtubule assembly, which is an essential step in neurogenesis. The product of this gene is a precursor poly

Alternate Names

DKFZp686E1099, DKFZp686F1345, FLJ38954, FUTSCH, MAP-1B, MAP5, microtubule-associated protein 1B

Gene Symbol

MAP1B

Additional MAP1B Products

Product Documents for MAP1B Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for MAP1B Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for MAP1B Antibody - BSA Free

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Protocols

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