MAPKAPK2 Antibody (2B3) - Azide and BSA Free

Novus Biologicals | Catalog # H00009261-M01

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Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, ELISA, Immunocytochemistry/ Immunofluorescence, Simple Western, Immunoprecipitation, Proximity Ligation Assay

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2b Kappa Clone # 2B3

Format

Azide and BSA Free
View all MAPKAPK2 Primary Antibodies »

Product Specifications

Immunogen

MAPKAPK2 (NP_116584, 302 a.a. ~ 400 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. IRNLLKTEPTQRMTITEFMNHPWIMQSTKVPQTPLHTSRVLKEDKERWEDVKEEMTSALATMRVDYEQIKIKKIEDASNPLLLKRRKKARALEAAALAH

Specificity

MAPKAPK2 - mitogen-activated protein kinase-activated protein kinase 2

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2b Kappa

Scientific Data Images for MAPKAPK2 Antibody (2B3) - Azide and BSA Free

Western Blot: MAPKAPK2 Antibody (2B3) [H00009261-M01]

Western Blot: MAPKAPK2 Antibody (2B3) [H00009261-M01]

Western Blot: MAPKAPK2 Antibody (2B3) [H00009261-M01] - MAPKAPK2 monoclonal antibody (M01), clone 2B3 Analysis of MAPKAPK2 expression in K-562.
Immunocytochemistry/ Immunofluorescence: MAPKAPK2 Antibody (2B3) [H00009261-M01]

Immunocytochemistry/ Immunofluorescence: MAPKAPK2 Antibody (2B3) [H00009261-M01]

Immunocytochemistry/Immunofluorescence: MAPKAPK2 Antibody (2B3) [H00009261-M01] - Analysis of monoclonal antibody to MAPKAPK2 on HeLa cell. Antibody concentration 10 ug/ml.
Immunohistochemistry-Paraffin: MAPKAPK2 Antibody (2B3) [H00009261-M01]

Immunohistochemistry-Paraffin: MAPKAPK2 Antibody (2B3) [H00009261-M01]

Immunohistochemistry-Paraffin: MAPKAPK2 Antibody (2B3) [H00009261-M01] - Analysis of monoclonal antibody to MAPKAPK2 on formalin-fixed paraffin-embedded human kidney. Antibody concentration 3 ug/ml.
Proximity Ligation Assay: MAPKAPK2 Antibody (2B3) [H00009261-M01]

Proximity Ligation Assay: MAPKAPK2 Antibody (2B3) [H00009261-M01]

Proximity Ligation Assay: MAPKAPK2 Antibody (2B3) [H00009261-M01] - Analysis of protein-protein interactions between AKT1 and MAPKAPK2. HeLa cells were stained with anti-AKT1 rabbit purified polyclonal 1:1200 and anti-MAPKAPK2 mouse monoclonal antibody 1:50. Each red dot represents the detection of protein-protein interaction complex, and nuclei were counterstained with DAPI (blue).
Immunoprecipitation: MAPKAPK2 Antibody (2B3) [H00009261-M01]

Immunoprecipitation: MAPKAPK2 Antibody (2B3) [H00009261-M01]

Immunoprecipitation: MAPKAPK2 Antibody (2B3) [H00009261-M01] - Analysis of MAPKAPK2 transfected lysate using anti-MAPKAPK2 monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with MAPKAPK2 MaxPab rabbit polyclonal antibody.
ELISA: MAPKAPK2 Antibody (2B3) [H00009261-M01]

ELISA: MAPKAPK2 Antibody (2B3) [H00009261-M01]

ELISA: MAPKAPK2 Antibody (2B3) [H00009261-M01] - Detection limit for recombinant GST tagged MAPKAPK2 is approximately 0.1ng/ml as a capture antibody.

Applications for MAPKAPK2 Antibody (2B3) - Azide and BSA Free

Application
Recommended Usage

ELISA

1:100-1:2000

Immunocytochemistry/ Immunofluorescence

1:10-1:500

Immunohistochemistry

1:10-1:500

Immunohistochemistry-Paraffin

1:10-1:500

Immunoprecipitation

1:10-1:500

Simple Western

1:50

Western Blot

1:500
Application Notes

Antibody reactive against cell lysate and recombinant protein for Western Blot. Has also been used for immunofluoresence, immunohistochemistry (paraffin), and ELISA.In Simple Western only 10-15 uL of the recommended dilution is used per data point. See Simple Western Antibody Database for Simple Western validation: separated by size, charge; antibody dilution of 1:50; detects a band at 50 kDa.

Reviewed Applications

Read 1 review rated 4 using H00009261-M01 in the following applications:

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: MAPKAPK2

This gene encodes a member of the Ser/Thr protein kinase family. This kinase is regulated through direct phosphorylation by p38 MAP kinase. In conjunction with p38 MAP kinase, this kinase is known to be involved in many cellular processes including stress and inflammatory responses, nuclear export, gene expression regulation and cell proliferation. Heat shock protein HSP27 was shown to be one of the substrates of this kinase in vivo. Two transcript variants encoding two different isoforms have been found for this gene.

Long Name

Mitogen-Activated Protein Kinase-Activated Protein Kinase 2

Alternate Names

MK2, Rps6kc1

Entrez Gene IDs

9261 (Human)

Gene Symbol

MAPKAPK2

OMIM

602006 (Human)

UniProt

NP_116584 (Human)

Additional MAPKAPK2 Products

Product Documents for MAPKAPK2 Antibody (2B3) - Azide and BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for MAPKAPK2 Antibody (2B3) - Azide and BSA Free

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

Interferons

Customer Reviews for MAPKAPK2 Antibody (2B3) - Azide and BSA Free (1)

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  • MAPKAPK2 Antibody (2B3)
    Name: Anonymous
    Application: Western Blot
    Sample Tested: Human breast cancer cell lines
    Species: Human
    Verified Customer | Posted 10/24/2018
    Human breast cancer cell line MDA-MB-231 was treated with paclitaxel for 72 hours and the expression of p-MK2 and total MK2 was detected by western blot.
    MAPKAPK2 Antibody (2B3) - Azide and BSA Free H00009261-M01

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Protocols

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FAQs

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Associated Pathways

VEGF - VEGF R2 Signaling Pathways VEGF - VEGF R2 Signaling Pathway Thumbnail