MAT2A Antibody - BSA Free
Novus Biologicals | Catalog # NBP1-92100
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Key Product Details
Species Reactivity
Validated:
Human, Mouse
Cited:
Human, Mouse
Predicted:
Rat (100%). Backed by our 100% Guarantee.
Applications
Validated:
Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence
Cited:
Immunohistochemistry-Paraffin, Western Blot, IF/IHC
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Format
BSA Free
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Product Specifications
Immunogen
This antibody was developed against Recombinant Protein corresponding to amino acids: MNGQLNGFHEAFIEEGTFL
Reactivity Notes
Mouse reactivity reported in scientific literature (PMID: 30926424).
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for MAT2A Antibody - BSA Free
Western Blot: MAT2A Antibody [NBP1-92100]
Western Blot: MAT2A Antibody [NBP1-92100] - Analysis in human cell line CAPAN-2.Immunocytochemistry/ Immunofluorescence: MAT2A Antibody [NBP1-92100]
Immunocytochemistry/Immunofluorescence: MAT2A Antibody [NBP1-92100] - Staining of human cell line U-251 MG shows localization to nucleoplasm. Antibody staining is shown in green.Immunohistochemistry-Paraffin: MAT2A Antibody [NBP1-92100]
Immunohistochemistry-Paraffin: MAT2A Antibody [NBP1-92100] - Staining of human Liver shows very weak nuclear positivity in hepatocytes.Immunohistochemistry-Paraffin: MAT2A Antibody [NBP1-92100]
Immunohistochemistry-Paraffin: MAT2A Antibody [NBP1-92100] - Staining of human Duodenum shows strong nuclear positivity in glandular cells.Immunohistochemistry-Paraffin: MAT2A Antibody [NBP1-92100]
Immunohistochemistry-Paraffin: MAT2A Antibody [NBP1-92100] - Staining of human Kidney shows strong nuclear positivity in cells in tubules.Immunohistochemistry-Paraffin: MAT2A Antibody [NBP1-92100]
Immunohistochemistry-Paraffin: MAT2A Antibody [NBP1-92100] - Staining of human Prostate shows strong nuclear positivity in glandular cells.Western Blot: MAT2A Antibody - BSA Free [NBP1-92100]
Analysis in human cell line CAPAN-2.Western Blot: MAT2A Antibody - BSA Free [NBP1-92100] -
Effects of MAT2A on learning and memory in 5 × FAD mice. (A,B) Expression of MAT2A protein in the hippocampus of 5 × FAD mice (n = 5). (C) Flow chart of injection of overexpressed MAT2A virus and subsequent neurobehavioral experiments. (D) Schematic representation of stereoscopic fluorescence diffusion in 5 × FAD mice hippocampal brain (scale bar = 500 μm). (E,F) Expression level of MAT2A protein after overexpression of MAT2A in 5 × FAD mice (n = 5). (G) Expression level of MAT2A mRNA after overexpression of MAT2A in 5 × FAD mice (n = 3). (H,I) NOR was performed to assess recognition memory after overexpression of MAT2A in 5 × FAD mice (n = 10). (J–L) YM was performed to assess spatial memory after overexpression of MAT2A in 5 × FAD mice (n = 10). (M–P) MWM was performed to assess spatial memory after overexpression of MAT2A in 5 × FAD mice (n = 10). Data are shown as the mean +/- SD. *p < 0.05, **p < 0.01, and ***p < 0.001. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/40308894), licensed under a CC-BY license. Not internally tested by Novus Biologicals.Western Blot: MAT2A Antibody - BSA Free [NBP1-92100] -
Effects of METTL16 regulation of MAT2A on learning and memory in 5 × FAD mice. (A,B) Expression of MAT2A protein in the hippocampus of 5 × FAD mice after METTL16 overexpression (n = 5). (C) Expression of MAT2A mRNA in the hippocampus of 5 × FAD mice after METTL16 overexpression (n = 3). (D) m6A methylation level of MAT2A mRNA in the hippocampus of 5 × FAD mice after METTL16 overexpression (n = 3). (E) Flow chart illustrating injection of overexpressed METTL16, knockdown MAT2A virus, and subsequent neurobehavioral experiments. (F) Schematic representation of stereoscopic fluorescence diffusion in 5 × FAD mice hippocampal brain (scale bar = 500 μm). (G,H) Expression level of MAT2A protein after overexpression of METTL16 and knockdown of MAT2A in 5 × FAD mice (n = 4). (I) Expression level of MAT2A mRNA after overexpression of METTL16 and knockdown of MAT2A in 5 × FAD mice (n = 3). (J,K) NOR was performed to assess recognition memory after overexpression of METTL16 and knockdown of MAT2A in 5 × FAD mice (n = 10). (L–N) YM was performed to assess spatial memory after overexpression of METTL16 and knockdown of MAT2A in 5 × FAD mice (n = 10). (O–R) MWM was performed to assess spatial memory after overexpression of METTL16 and knockdown of MAT2A in 5 × FAD mice (n = 10). Data are shown as the mean +/- SD. *p < 0.05, **p < 0.01, and ***p < 0.001. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/40308894), licensed under a CC-BY license. Not internally tested by Novus Biologicals.Western Blot: MAT2A Antibody - BSA Free [NBP1-92100] -
Effects of MAT2A on learning and memory in 5 × FAD mice. (A,B) Expression of MAT2A protein in the hippocampus of 5 × FAD mice (n = 5). (C) Flow chart of injection of overexpressed MAT2A virus and subsequent neurobehavioral experiments. (D) Schematic representation of stereoscopic fluorescence diffusion in 5 × FAD mice hippocampal brain (scale bar = 500 μm). (E,F) Expression level of MAT2A protein after overexpression of MAT2A in 5 × FAD mice (n = 5). (G) Expression level of MAT2A mRNA after overexpression of MAT2A in 5 × FAD mice (n = 3). (H,I) NOR was performed to assess recognition memory after overexpression of MAT2A in 5 × FAD mice (n = 10). (J–L) YM was performed to assess spatial memory after overexpression of MAT2A in 5 × FAD mice (n = 10). (M–P) MWM was performed to assess spatial memory after overexpression of MAT2A in 5 × FAD mice (n = 10). Data are shown as the mean +/- SD. *p < 0.05, **p < 0.01, and ***p < 0.001. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/40308894), licensed under a CC-BY license. Not internally tested by Novus Biologicals.Western Blot: MAT2A Antibody - BSA Free [NBP1-92100] -
Effects of METTL16 regulation of MAT2A on learning and memory in 5 × FAD mice. (A,B) Expression of MAT2A protein in the hippocampus of 5 × FAD mice after METTL16 overexpression (n = 5). (C) Expression of MAT2A mRNA in the hippocampus of 5 × FAD mice after METTL16 overexpression (n = 3). (D) m6A methylation level of MAT2A mRNA in the hippocampus of 5 × FAD mice after METTL16 overexpression (n = 3). (E) Flow chart illustrating injection of overexpressed METTL16, knockdown MAT2A virus, and subsequent neurobehavioral experiments. (F) Schematic representation of stereoscopic fluorescence diffusion in 5 × FAD mice hippocampal brain (scale bar = 500 μm). (G,H) Expression level of MAT2A protein after overexpression of METTL16 and knockdown of MAT2A in 5 × FAD mice (n = 4). (I) Expression level of MAT2A mRNA after overexpression of METTL16 and knockdown of MAT2A in 5 × FAD mice (n = 3). (J,K) NOR was performed to assess recognition memory after overexpression of METTL16 and knockdown of MAT2A in 5 × FAD mice (n = 10). (L–N) YM was performed to assess spatial memory after overexpression of METTL16 and knockdown of MAT2A in 5 × FAD mice (n = 10). (O–R) MWM was performed to assess spatial memory after overexpression of METTL16 and knockdown of MAT2A in 5 × FAD mice (n = 10). Data are shown as the mean +/- SD. *p < 0.05, **p < 0.01, and ***p < 0.001. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/40308894), licensed under a CC-BY license. Not internally tested by Novus Biologicals.Immunohistochemistry: MAT2A Antibody - BSA Free [NBP1-92100] -
MAT2A and PDCD6 are highly expressed in cervical cancer tissues and are correlated with poor prognosis with cervical cancer patients.A Immunohistochemical staining with MAT2A antibody was performed in 67 cervical cancer specimens and 67 normal cervical specimens. Representative photos of tumors versus the normal tissue and adjacent normal tissues were shown (magnification: ×100 and ×400, left panel). Comparative analysis of MAT2A expression among normal cervical tissue, adjacent normal tissue and cervical cancer specimen was shown (right panel).***P < 0.001. Scar bars: 50 μM. B Immunohistochemical staining with PDCD6 antibody was performed in 67 cervical cancer specimens and 67 normal cervical specimens. Representative photos of tumors versus the normal tissue and adjacent normal tissue was shown (magnification: ×100 and ×400, left panel). Comparative analysis of PDCD6 expression among normal cervical tissue, adjacent normal tissue and cervical cancer specimen was shown (right panel). ***P < 0.001. Scar bars: 50 μM. C Semiquantitative scoring and correlation analysis indicating the correlation between MAT2A and PDCD6 (r = 0.819, P < 0.001). D Prognostic analysis of PDCD6 gene expression in cervical cancer patients (GEPIA2) was illustrated. Overall survival (OS) and disease-free survival (DFS) analysis were performed to show the survival status in the TCGA cohort via GEPIA2. Kaplan–Meier curves were plotted with P-values and HRs by log-rank tests and Cox regression models. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/35396512), licensed under a CC-BY license. Not internally tested by Novus Biologicals.Applications for MAT2A Antibody - BSA Free
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
0.25-2 ug/ml
Immunohistochemistry
1:200 - 1:500
Immunohistochemistry-Paraffin
1:200 - 1:500
Western Blot
0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval method is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.
Formulation, Preparation, and Storage
Purification
Affinity purified
Formulation
PBS (pH 7.2) and 40% Glycerol
Format
BSA Free
Preservative
0.02% Sodium Azide
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: MAT2A
MAT1A and MAT2A antibodies are useful tools for cancer research and liver function research.
Long Name
Methionine Adenosyltransferase 2A
Alternate Names
MAT-II, MATA2, SAMS2
Entrez Gene IDs
4144 (Human)
Gene Symbol
MAT2A
UniProt
Additional MAT2A Products
Product Documents for MAT2A Antibody - BSA Free
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for MAT2A Antibody - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Citations for MAT2A Antibody - BSA Free
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Antigen Retrieval Protocol (PIER)
- Antigen Retrieval for Frozen Sections Protocol
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- Chromogenic IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Chromogenic Immunohistochemistry Staining of Frozen Tissue
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- Fluorescent IHC Staining of Frozen Tissue Protocol
- Graphic Protocol for Heat-induced Epitope Retrieval
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Graphic Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- ICC Cell Smear Protocol for Suspension Cells
- ICC Immunocytochemistry Protocol Videos
- ICC for Adherent Cells
- IHC Sample Preparation (Frozen sections vs Paraffin)
- Immunocytochemistry (ICC) Protocol
- Immunocytochemistry Troubleshooting
- Immunofluorescence of Organoids Embedded in Cultrex Basement Membrane Extract
- Immunofluorescent IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunohistochemistry Frozen Troubleshooting
- Immunohistochemistry Paraffin Troubleshooting
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Protocol for Heat-Induced Epitope Retrieval (HIER)
- Protocol for Making a 4% Formaldehyde Solution in PBS
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Fluorescent ICC Staining of Cell Smears - Graphic
- Protocol for the Fluorescent ICC Staining of Cultured Cells on Coverslips - Graphic
- Protocol for the Preparation & Fixation of Cells on Coverslips
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections - Graphic
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections - Graphic
- Protocol for the Preparation and Fluorescent ICC Staining of Cells on Coverslips
- Protocol for the Preparation and Fluorescent ICC Staining of Non-adherent Cells
- Protocol for the Preparation and Fluorescent ICC Staining of Stem Cells on Coverslips
- Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- Protocol for the Preparation of a Cell Smear for Non-adherent Cell ICC - Graphic
- R&D Systems Quality Control Western Blot Protocol
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: Immunohistochemistry
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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