MAT2A Antibody - BSA Free

Novus Biologicals | Catalog # NBP1-92100

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human, Mouse

Cited:

Human, Mouse

Predicted:

Rat (100%). Backed by our 100% Guarantee.

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence

Cited:

Immunohistochemistry-Paraffin, Western Blot, IF/IHC

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
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Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: MNGQLNGFHEAFIEEGTFL

Reactivity Notes

Mouse reactivity reported in scientific literature (PMID: 30926424).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for MAT2A Antibody - BSA Free

Western Blot: MAT2A Antibody [NBP1-92100]

Western Blot: MAT2A Antibody [NBP1-92100]

Western Blot: MAT2A Antibody [NBP1-92100] - Analysis in human cell line CAPAN-2.
Immunocytochemistry/ Immunofluorescence: MAT2A Antibody [NBP1-92100]

Immunocytochemistry/ Immunofluorescence: MAT2A Antibody [NBP1-92100]

Immunocytochemistry/Immunofluorescence: MAT2A Antibody [NBP1-92100] - Staining of human cell line U-251 MG shows localization to nucleoplasm. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: MAT2A Antibody [NBP1-92100]

Immunohistochemistry-Paraffin: MAT2A Antibody [NBP1-92100]

Immunohistochemistry-Paraffin: MAT2A Antibody [NBP1-92100] - Staining of human Liver shows very weak nuclear positivity in hepatocytes.
Immunohistochemistry-Paraffin: MAT2A Antibody [NBP1-92100]

Immunohistochemistry-Paraffin: MAT2A Antibody [NBP1-92100]

Immunohistochemistry-Paraffin: MAT2A Antibody [NBP1-92100] - Staining of human Duodenum shows strong nuclear positivity in glandular cells.
Immunohistochemistry-Paraffin: MAT2A Antibody [NBP1-92100]

Immunohistochemistry-Paraffin: MAT2A Antibody [NBP1-92100]

Immunohistochemistry-Paraffin: MAT2A Antibody [NBP1-92100] - Staining of human Kidney shows strong nuclear positivity in cells in tubules.
Immunohistochemistry-Paraffin: MAT2A Antibody [NBP1-92100]

Immunohistochemistry-Paraffin: MAT2A Antibody [NBP1-92100]

Immunohistochemistry-Paraffin: MAT2A Antibody [NBP1-92100] - Staining of human Prostate shows strong nuclear positivity in glandular cells.
MAT2A Antibody - BSA Free Western Blot: MAT2A Antibody - BSA Free [NBP1-92100]

Western Blot: MAT2A Antibody - BSA Free [NBP1-92100]

Analysis in human cell line CAPAN-2.
MAT2A Antibody - BSA Free

Western Blot: MAT2A Antibody - BSA Free [NBP1-92100] -

Effects of MAT2A on learning and memory in 5 × FAD mice. (A,B) Expression of MAT2A protein in the hippocampus of 5 × FAD mice (n = 5). (C) Flow chart of injection of overexpressed MAT2A virus and subsequent neurobehavioral experiments. (D) Schematic representation of stereoscopic fluorescence diffusion in 5 × FAD mice hippocampal brain (scale bar = 500 μm). (E,F) Expression level of MAT2A protein after overexpression of MAT2A in 5 × FAD mice (n = 5). (G) Expression level of MAT2A mRNA after overexpression of MAT2A in 5 × FAD mice (n = 3). (H,I) NOR was performed to assess recognition memory after overexpression of MAT2A in 5 × FAD mice (n = 10). (J–L) YM was performed to assess spatial memory after overexpression of MAT2A in 5 × FAD mice (n = 10). (M–P) MWM was performed to assess spatial memory after overexpression of MAT2A in 5 × FAD mice (n = 10). Data are shown as the mean +/- SD. *p < 0.05, **p < 0.01, and ***p < 0.001. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/40308894), licensed under a CC-BY license. Not internally tested by Novus Biologicals.
MAT2A Antibody - BSA Free

Western Blot: MAT2A Antibody - BSA Free [NBP1-92100] -

Effects of METTL16 regulation of MAT2A on learning and memory in 5 × FAD mice. (A,B) Expression of MAT2A protein in the hippocampus of 5 × FAD mice after METTL16 overexpression (n = 5). (C) Expression of MAT2A mRNA in the hippocampus of 5 × FAD mice after METTL16 overexpression (n = 3). (D) m6A methylation level of MAT2A mRNA in the hippocampus of 5 × FAD mice after METTL16 overexpression (n = 3). (E) Flow chart illustrating injection of overexpressed METTL16, knockdown MAT2A virus, and subsequent neurobehavioral experiments. (F) Schematic representation of stereoscopic fluorescence diffusion in 5 × FAD mice hippocampal brain (scale bar = 500 μm). (G,H) Expression level of MAT2A protein after overexpression of METTL16 and knockdown of MAT2A in 5 × FAD mice (n = 4). (I) Expression level of MAT2A mRNA after overexpression of METTL16 and knockdown of MAT2A in 5 × FAD mice (n = 3). (J,K) NOR was performed to assess recognition memory after overexpression of METTL16 and knockdown of MAT2A in 5 × FAD mice (n = 10). (L–N) YM was performed to assess spatial memory after overexpression of METTL16 and knockdown of MAT2A in 5 × FAD mice (n = 10). (O–R) MWM was performed to assess spatial memory after overexpression of METTL16 and knockdown of MAT2A in 5 × FAD mice (n = 10). Data are shown as the mean +/- SD. *p < 0.05, **p < 0.01, and ***p < 0.001. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/40308894), licensed under a CC-BY license. Not internally tested by Novus Biologicals.
MAT2A Antibody - BSA Free

Western Blot: MAT2A Antibody - BSA Free [NBP1-92100] -

Effects of MAT2A on learning and memory in 5 × FAD mice. (A,B) Expression of MAT2A protein in the hippocampus of 5 × FAD mice (n = 5). (C) Flow chart of injection of overexpressed MAT2A virus and subsequent neurobehavioral experiments. (D) Schematic representation of stereoscopic fluorescence diffusion in 5 × FAD mice hippocampal brain (scale bar = 500 μm). (E,F) Expression level of MAT2A protein after overexpression of MAT2A in 5 × FAD mice (n = 5). (G) Expression level of MAT2A mRNA after overexpression of MAT2A in 5 × FAD mice (n = 3). (H,I) NOR was performed to assess recognition memory after overexpression of MAT2A in 5 × FAD mice (n = 10). (J–L) YM was performed to assess spatial memory after overexpression of MAT2A in 5 × FAD mice (n = 10). (M–P) MWM was performed to assess spatial memory after overexpression of MAT2A in 5 × FAD mice (n = 10). Data are shown as the mean +/- SD. *p < 0.05, **p < 0.01, and ***p < 0.001. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/40308894), licensed under a CC-BY license. Not internally tested by Novus Biologicals.
MAT2A Antibody - BSA Free

Western Blot: MAT2A Antibody - BSA Free [NBP1-92100] -

Effects of METTL16 regulation of MAT2A on learning and memory in 5 × FAD mice. (A,B) Expression of MAT2A protein in the hippocampus of 5 × FAD mice after METTL16 overexpression (n = 5). (C) Expression of MAT2A mRNA in the hippocampus of 5 × FAD mice after METTL16 overexpression (n = 3). (D) m6A methylation level of MAT2A mRNA in the hippocampus of 5 × FAD mice after METTL16 overexpression (n = 3). (E) Flow chart illustrating injection of overexpressed METTL16, knockdown MAT2A virus, and subsequent neurobehavioral experiments. (F) Schematic representation of stereoscopic fluorescence diffusion in 5 × FAD mice hippocampal brain (scale bar = 500 μm). (G,H) Expression level of MAT2A protein after overexpression of METTL16 and knockdown of MAT2A in 5 × FAD mice (n = 4). (I) Expression level of MAT2A mRNA after overexpression of METTL16 and knockdown of MAT2A in 5 × FAD mice (n = 3). (J,K) NOR was performed to assess recognition memory after overexpression of METTL16 and knockdown of MAT2A in 5 × FAD mice (n = 10). (L–N) YM was performed to assess spatial memory after overexpression of METTL16 and knockdown of MAT2A in 5 × FAD mice (n = 10). (O–R) MWM was performed to assess spatial memory after overexpression of METTL16 and knockdown of MAT2A in 5 × FAD mice (n = 10). Data are shown as the mean +/- SD. *p < 0.05, **p < 0.01, and ***p < 0.001. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/40308894), licensed under a CC-BY license. Not internally tested by Novus Biologicals.
MAT2A Antibody - BSA Free

Immunohistochemistry: MAT2A Antibody - BSA Free [NBP1-92100] -

MAT2A and PDCD6 are highly expressed in cervical cancer tissues and are correlated with poor prognosis with cervical cancer patients.A Immunohistochemical staining with MAT2A antibody was performed in 67 cervical cancer specimens and 67 normal cervical specimens. Representative photos of tumors versus the normal tissue and adjacent normal tissues were shown (magnification: ×100 and ×400, left panel). Comparative analysis of MAT2A expression among normal cervical tissue, adjacent normal tissue and cervical cancer specimen was shown (right panel).***P < 0.001. Scar bars: 50 μM. B Immunohistochemical staining with PDCD6 antibody was performed in 67 cervical cancer specimens and 67 normal cervical specimens. Representative photos of tumors versus the normal tissue and adjacent normal tissue was shown (magnification: ×100 and ×400, left panel). Comparative analysis of PDCD6 expression among normal cervical tissue, adjacent normal tissue and cervical cancer specimen was shown (right panel). ***P < 0.001. Scar bars: 50 μM. C Semiquantitative scoring and correlation analysis indicating the correlation between MAT2A and PDCD6 (r = 0.819, P < 0.001). D Prognostic analysis of PDCD6 gene expression in cervical cancer patients (GEPIA2) was illustrated. Overall survival (OS) and disease-free survival (DFS) analysis were performed to show the survival status in the TCGA cohort via GEPIA2. Kaplan–Meier curves were plotted with P-values and HRs by log-rank tests and Cox regression models. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/35396512), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Applications for MAT2A Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:200 - 1:500

Immunohistochemistry-Paraffin

1:200 - 1:500

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval method is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: MAT2A

MAT1A and MAT2A are catalytic subunits of methionine adenosyltransferase (MAT), which catalyzes the formation of S-adenosyltransferase for methionine catabolism. Inactivation of MAT1A has been linked to liver disease. In addition, in certain liver cancers, expression of MAT1A is replaced by expression of MAT1B. Overexpression of MAT2A has also been observed in colon cancer, leading to the proposal that MAT2A facilitates cancer cell growth.

MAT1A and MAT2A antibodies are useful tools for cancer research and liver function research.

Long Name

Methionine Adenosyltransferase 2A

Alternate Names

MAT-II, MATA2, SAMS2

Entrez Gene IDs

4144 (Human)

Gene Symbol

MAT2A

UniProt

Additional MAT2A Products

Product Documents for MAT2A Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for MAT2A Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for MAT2A Antibody - BSA Free

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Protocols

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