Matrin 3 Antibody

Novus Biologicals | Catalog # NB100-1761

Novus Biologicals
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Key Product Details

Validated by

Knockout/Knockdown

Species Reactivity

Validated:

Human, Mouse

Cited:

Human, Mouse

Applications

Validated:

Knockout Validated, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation

Cited:

Western Blot, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation, Knockdown Validated

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG
View all Matrin 3 Primary Antibodies »

Product Specifications

Immunogen

The immunogen recognized by this antibody maps to a region between residue 800 and the C-terminus (residue 847) of human Matrin 3 using the numbering given in entry NP_061322.2 (GeneID 9782).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Matrin 3 Antibody

Matrin 3 Antibody

Western Blot Shows Human Matrin 3 Specificity Using Knockout Cell Line.

Western blot shows lysates of HAP1 human near-haploid cell line and Matrin 3 knockout HAP1 cell line (KO). Nitrocellulose membrane was probed with Matrin 3 Antibody (Catalog # NB100-1761) O/N at 4C, followed by HRP-conjugated Secondary Antibody and ECL detection. A specific band was detected for Matrin 3 (as indicated) in the parental HAP1 cell line, but is not detectable in knockout HAP1 cell line. Primary antibody dilution used: 1/5000. The Ponceau stained transfers of each blot are shown. Image, protocol and testing courtesy of YCharOS Inc. (ycharos.com).
Matrin 3 Antibody

Detection of Matrin 3 by Immunoprecipitation.

HAP1 lysates were prepared, and immunoprecipitation was performed using 2.0 ug of Matrin 3 antibody (Catalog # NBP1-04340) pre-coupled to Dynabeads protein A. Samples were washed and processed for Western Blot with Matrin 3 antibody. For Western Blot, Rb x Matrin 3 was used at 1/500. The Ponceau stained transfers of each blot are shown. SM=4% starting material; UB=4% unbound fraction; IP=immunoprecipitate. Image, protocol and testing courtesy of YCharOS Inc. (ycharos.com).
Immunoprecipitation: Matrin 3 Antibody [NB100-1761]

Immunoprecipitation: Matrin 3 Antibody [NB100-1761]

Immunoprecipitation: Matrin 3 Antibody [NB100-1761] - Detection of Human and Mouse Matrin 3 on HeLa whole cell lysate using NB100-1761. Matrin 3 was also immunoprecipitated using another rabbit anti-Matrin 3 antibody and NB100-1559.
Immunohistochemistry: Matrin 3 Antibody [NB100-1761]

Immunohistochemistry: Matrin 3 Antibody [NB100-1761]

Immunohistochemistry: Matrin 3 Antibody [NB100-1761] - Sample: FFPE section of human stomach carcinoma. Antibody: Affinity purified rabbit anti-Matrin 3 used at a dilution of 1:400 (0.5ug/ml). Detection: Red-fluorescent Goat anti-Rabbit IgG-heavy and light chain cross-adsorbed Antibody DyLight 594 Conjugated
Immunohistochemistry-Paraffin: Matrin 3 Antibody [NB100-1761]

Immunohistochemistry-Paraffin: Matrin 3 Antibody [NB100-1761]

Immunohistochemistry-Paraffin: Matrin 3 Antibody [NB100-1761] - Section of human laryengeal squamous cell carcinoma (left) and mouse squamous cell carcinoma (right). Antibody: Affinity purified rabbit anti- Matrin 3 used at a dilution of 1:1,000 (0.2ug/ml). Detection: DAB
Western Blot: Matrin 3 Antibody [NB100-1761]

Western Blot: Matrin 3 Antibody [NB100-1761]

Matrin-3-Antibody-Western-Blot-NB100-1761-img0017.jpg
Immunohistochemistry: Matrin 3 Antibody [NB100-1761]

Immunohistochemistry: Matrin 3 Antibody [NB100-1761]

Matrin-3-Antibody-Immunohistochemistry-NB100-1761-img0020.jpg
Immunohistochemistry: Matrin 3 Antibody [NB100-1761]

Immunohistochemistry: Matrin 3 Antibody [NB100-1761]

Matrin-3-Antibody-Immunohistochemistry-NB100-1761-img0018.jpg
Immunohistochemistry: Matrin 3 Antibody [NB100-1761]

Immunohistochemistry: Matrin 3 Antibody [NB100-1761]

Matrin-3-Antibody-Immunohistochemistry-NB100-1761-img0019.jpg
Immunohistochemistry: Matrin 3 Antibody [NB100-1761]

Immunohistochemistry: Matrin 3 Antibody [NB100-1761]

Matrin-3-Antibody-Immunohistochemistry-NB100-1761-img0021.jpg
Immunoprecipitation: Matrin 3 Antibody [NB100-1761]

Immunoprecipitation: Matrin 3 Antibody [NB100-1761]

Immunoprecipitation: Matrin 3 Antibody [NB100-1761] - IP of human fibroblasts. Image from verified customer review.
Matrin 3 Antibody

Knockout Validation of Matrin 3 Antibody in HAP1 and Matrin 3 Knockout HAP1 Cells in Immunocytochemistry/Immunofluorescence.

HAP1 WT and Matrin 3 KO cells were labelled with a green or a far-red fluorescent dye, respectively. Cells were stained with Rab1A antibody and with the corresponding Alexa-fluor 555 coupled secondary antibody including DAPI. Acquisition of the blue (nucleus-DAPI), green (identification of WT cells), red (antibody staining) and far-red (identification of KO cells) channels was performed. Representative images of the blue and red (grayscale) channels are shown. WT and KO cells are outlined with green and magenta dashed line, respectively. Antibody dilution used: 1/200. Image, protocol and testing courtesy of YCharOS Inc. (ycharos.com).
Matrin 3 Antibody

Western Blot: Matrin 3 Antibody [NB100-1761] -

Western Blot: Matrin 3 Antibody [NB100-1761] - TRAP150 inhibits the RRM-dependent binding activity of PSF, & inhibition of binding requires more than the minimal RRM binding domain. (A) UV crosslinking of ESS1 RNA with full-length PSF (PSF-FL) either alone (-), in the presence of BSA as a control or in the presence of increasing amounts of full-length FLAG-tagged TRAP150. (B) Same as panel (A), but competing binding of exRRMs of PSF with indicated truncations of GST-TRAP150 or GST alone. (C) Same as panel (A) but competing for binding of the minRRMs & hnRNP L by GST-TRAP(PID). (D) Western blots of immunoprecipitation of PSF from unstimulated (TRAP150 bound) & stimulated (TRAP150 unbound) JSL1 cells showing relative binding of TRAP150 & other known PSF-interacting partners. The source of the doublet for PSPC1 & p54nrb/NONO in stimulated cells is unknown, but is the same in input & IP samples. Image collected & cropped by CiteAb from the following publication (https://academic.oup.com/nar/article-lookup/doi/10.1093/nar/gkv816), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Applications for Matrin 3 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:250-1:1000

Immunohistochemistry

1:10-1:500

Immunohistochemistry-Paraffin

1:10-1:500

Immunoprecipitation

1-4 ug/mg of lysate

Western Blot

1:5000-1:15000
Application Notes
Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections.

Reviewed Applications

Read 1 review rated 5 using NB100-1761 in the following applications:

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

TBS and 0.1% BSA

Preservative

0.09% Sodium Azide

Concentration

0.2 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: Matrin 3

Matrin 3 is a nuclear matrix protein that has been observed to mediate multiple cellular processes. Matrin 3 can function to withhold defective RNAs within the nucleus by anchoring them to the nuclear matrix and modulate the promoter activity of genes proximal to matrix/scaffold attachment region (MAR/SAR). Matrin 3 has also been demonstrated to be the main PKA substrate following NMDA receptor activation in NMDA-induced neuronal cell death. Alternate names for Matrin 3 include MATR3, MGC9105, KIAA0723, DKFZp686K0542, and DKFZp686K23100.

Alternate Names

distal 2, DKFZp686K0542, DKFZp686K23100, matrin 3, matrin-3, vocal cord and pharyngeal weakness with distal myopathy

Entrez Gene IDs

9782 (Human); 17184 (Mouse)

Gene Symbol

MATR3

UniProt

P43243 (Human)

Additional Matrin 3 Products

Product Documents for Matrin 3 Antibody

Certificate of Analysis

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Product Specific Notices for Matrin 3 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for Matrin 3 Antibody

Customer Reviews for Matrin 3 Antibody (1)

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  • Name: Jennifer Fox
    Application: Immunoprecipitation
    Sample Tested: Human fibroblasts
    Species: Human
    Verified Customer | Posted 09/12/2014
    Matrin3 IP
    Matrin 3 Antibody NB100-1761

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Protocols

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