Matriptase 2 Antibody - BSA Free

Novus Biologicals | Catalog # NBP3-35158

Novus Biologicals
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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Western Blot, ELISA, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all Matriptase 2 Primary Antibodies »

Product Specifications

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 77-220 of human Matriptase 2 (NP_705837.1).

Sequence:
GYKAEVMVSQVYSGSLRVLNRHFSQDLTRRESSAFRSETAKAQKMLKELITSTRLGTYYNSSSVYSFGEGPLTCFFWFILQIPEHRRLMLSPEVVQALLVEELLSTVNSSAAVPYRAEYEVDPEGLVILEASVKDIAALNSTLG

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

90 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Matriptase 2 Antibody - BSA Free

Matriptase 2 Antibody

Western Blot: Matriptase 2 Antibody [NBP3-35158] -

Western Blot: Matriptase 2 Antibody [NBP3-35158] - Western blot analysis of various lysates using Matriptase 2 Rabbit pAb at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 90s.
Matriptase 2 Antibody

Western Blot: Matriptase 2 Antibody [NBP3-35158] -

Western Blot: Matriptase 2 Antibody [NBP3-35158] - Western blot analysis of lysates from Rat liver, using Matriptase 2 Rabbit pAb at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 180s.
Matriptase 2 Antibody

Immunocytochemistry/ Immunofluorescence: Matriptase 2 Antibody [NBP3-35158] -

Immunocytochemistry/ Immunofluorescence: Matriptase 2 Antibody [NBP3-35158] - Immunofluorescence analysis of paraffin-embedded mouse liver using Matriptase 2 Rabbit pAb at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Applications for Matriptase 2 Antibody - BSA Free

Application
Recommended Usage

ELISA

Recommended starting concentration is 1 ug/mL

Immunocytochemistry/ Immunofluorescence

1:50 - 1:200

Western Blot

1:500 - 1:1000

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.3), 50% glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Matriptase 2

The protein encoded by the Matriptase 2 gene is a type II transmembrane serine proteinase that is found attached to the cell surface. The encoded protein may be involved in matrix remodeling processes in the liver. [provided by RefSeq]

Alternate Names

EC 3.4.21, EC 3.4.21.-, FLJ30744, IRIDA, Matriptase-2, membrane-bound mosaic serine proteinase matriptase-2, transmembrane protease serine 6, transmembrane protease, serine 6, type II transmembrane serine protease 6

Gene Symbol

TMPRSS6

Additional Matriptase 2 Products

Product Documents for Matriptase 2 Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for Matriptase 2 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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