MAX binding protein Antibody (HL2309) - Azide and BSA Free

Novus Biologicals | Catalog # NBP3-25567

Recombinant Monoclonal Antibody
Novus Biologicals
100% Guarantee

Key Product Details

Species Reactivity

Human, Mouse

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # HL2309 expressed in HEK293

Format

Azide and BSA Free
View all MAX binding protein Primary Antibodies »

Product Specifications

Immunogen

Recombinant fragmemt of human MAX binding protein.

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for MAX binding protein Antibody (HL2309) - Azide and BSA Free

MAX binding protein Antibody (HL2309) - Azide and BSA Free

Western Blot: MAX binding protein Antibody (HL2309) - Azide and BSA Free [NBP3-25567] -

Western Blot: MAX binding protein Antibody (HL2309) - Azide and BSA Free [NBP3-25567] - A431 whole cell and nuclear extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with MNT antibody [HL2309] (NBP3-25567) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
MAX binding protein Antibody (HL2309) - Azide and BSA Free

Western Blot: MAX binding protein Antibody (HL2309) - Azide and BSA Free [NBP3-25567] -

Western Blot: MAX binding protein Antibody (HL2309) - Azide and BSA Free [NBP3-25567] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with MNT antibody [HL2309] (NBP3-25567) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.
MAX binding protein Antibody (HL2309) - Azide and BSA Free

Immunohistochemistry-Paraffin: MAX binding protein Antibody (HL2309) - Azide and BSA Free [NBP3-25567] -

Immunohistochemistry-Paraffin: MAX binding protein Antibody (HL2309) - Azide and BSA Free [NBP3-25567] - MNT antibody [HL2309] detects MNT protein at cell membrane and cytoplasm by immunohistochemical analysis. Sample: Paraffin-embedded mouse testis. MNT stained by MNT antibody [HL2309] (NBP3-25567) diluted at 1:200. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
MAX binding protein Antibody (HL2309) - Azide and BSA Free

Immunocytochemistry/Immunofluorescence: MAX binding protein Antibody (HL2309) - Azide and BSA Free [NBP3-25567] -

Immunocytochemistry/Immunofluorescence: MAX binding protein Antibody (HL2309) - Azide and BSA Free [NBP3-25567] - MNT antibody [HL2309] detects MNT protein at cytoplasm and nucleus by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: MNT stained by MNT antibody [HL2309] (NBP3-25567) diluted at 1:500. Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] diluted at 1:1000.

Applications for MAX binding protein Antibody (HL2309) - Azide and BSA Free

Application
Recommended Usage

Western Blot

1:500-1:3000

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: MAX binding protein

MNT, also known as MAX-binding protein MNT, is a 582 amino acid protein that is 62 kDa, has a basic-Helix-Loop-Helix-zipper domain (bHLHzip) with which it binds the canonical DNA sequence CANNTG, known as the E box, following heterodimerization with Max proteins. This protein is likely a transcriptional repressor and an antagonist of Myc-dependent transcriptional activation and cell growth and represses transcription by binding to DNA binding proteins at its N-terminal Sin3-interaction domain. Current research is being performed on several diseases and disorders including Miller-Dieker lissencephaly, Miller-Dieker syndrome, Williams-Beuren syndrome, tetanus neonatorum, rheumatic fever, lissencephaly, tetanus, cholestasis, rabies, medulloblastoma, lung cancer, neuroblastoma, and leukemia.

Alternate Names

BHLHD3, bHLHd3Max-interacting protein, Class D basic helix-loop-helix protein 3, MAD6, MAX binding protein, MXD6, Myc antagonist MNT, Protein ROX, ROXmax-binding protein MNT

Gene Symbol

MNT

Additional MAX binding protein Products

Product Documents for MAX binding protein Antibody (HL2309) - Azide and BSA Free

Certificate of Analysis

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Product Specific Notices for MAX binding protein Antibody (HL2309) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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