MBL-2 Antibody (14D12)

Novus Biologicals | Catalog # NBP1-60128

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Key Product Details

Species Reactivity

Mouse

Applications

Immunohistochemistry, Immunohistochemistry-Frozen, Western Blot, Immunoassay, Immunocytochemistry/ Immunofluorescence, Functional

Label

Unconjugated

Antibody Source

Monoclonal Rat IgG2A Clone # 14D12
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Product Specifications

Immunogen

Mouse MBL-C

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.

Clonality

Monoclonal

Host

Rat

Isotype

IgG2A

Description

Positive Control: Mouse Serum and kidney tissue

Scientific Data Images for MBL-2 Antibody (14D12)

Immunohistochemistry-Frozen: MBL-2 Antibody (14D12) [NBP1-60128]

Immunohistochemistry-Frozen: MBL-2 Antibody (14D12) [NBP1-60128]

Immunohistochemistry-Frozen: Antibody (mAb 14D12) - MBL-C (clone 14D12) deposition in developing murine atherosclerotic lesions following 10 weeks of high fat feeding. MBL-C was detected in and around invading macrophages invading the intima (insert). MBL-C bound, similar to MBL-A, at sites of necrosis (upper right corner). No MBL-C binding was shown in the media or on fibrous caps covering the thickened intima.

Applications for MBL-2 Antibody (14D12)

Application
Recommended Usage

Immunoassay

1 ug/ml

Immunocytochemistry/ Immunofluorescence

1:10-1:500

Immunohistochemistry

1:10-1:500

Immunohistochemistry-Frozen

1:10-1:500

Western Blot

1:100-1:2000
Application Notes
For IHC-F: Cryostat sections (7 um) of mouse tissue were acetone fixed and stained o/n at 4 degrees Celsius in TBS/2% goat serum. (Matthijsen, R et al). Immunoassay: Biotinylated 14D12 was used as detector in ELISA in TBS/Tween-20/Ca (see Liu, H et al). For IF: Hyphae of C. albicans were fixed on poly-l-lysin coated slides with ice-cold aceton. Slides were incubated for 1h at RT with 14D12 (see Held, K et al). For WB: On a 12% non-reducing SDS-PAGE a prominent band of ca. 50 kDa is detected. Under reducing conditions a 26 kDa band is detected (see Phaneuf, L et al).

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

0.2 um filtered solution in PBS and 0.1% BSA

Preservative

0.02% Sodium Azide

Concentration

0.1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C.

Background: MBL-2

Mannose binding lectin (MBL), also called mannose- or mannan-binding protein (MBP), is a member of the group of collectins. MBL is an important pattern-recognition receptor in the innate immune system. The protein mediates innate immune responses, such as activation of the complement lectin pathway and phagocytosis, to help fight infections. MBL is an oligomeric lectin that recognizes carbohydrates as mannose and N-acetylglucosamine on pathogens. MBL contains a cysteine rich, a collagen like and a carbohydrate recognition domain. Binding of MBL leads to the activation of MBL-associated serine proteases (MASP's). Activated MASP-2 cleaves C4 and C2 in a similar way as C1s do for the classical pathway (CP) leading to the formation of C4b2a, cleavage of the classical pathway convertase C3, and eventually complement activation up to the formation of the membrane attack complex. MBL is able to activate the complement pathway independent of the classical and alternative complement activation pathways. MBL is predominantly synthesized by hepatocytes and has been isolated from the liver or serum of several vertebrate species. Only one form of human MBL has been characterized, while two forms are found in rhesus monkeys, rabbits, rats and mice. The mouse forms are known as MBL-A and MBL-C. The MBL-C concentrations in serum are about 6-fold compared to that of MBL-A. MBL-A, but not MBL-C, was found to be an acute phase protein in casein and LPS-injection models. MBL-C exists in higher oligomeric forms than MBL-A. The monoclonal antibody 14D12 is a calcium-dependent antibody.

Long Name

Mannose Binding Lectin 2

Alternate Names

L-MBP, MBL-C

Gene Symbol

Mbl2

Additional MBL-2 Products

Product Documents for MBL-2 Antibody (14D12)

Certificate of Analysis

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Product Specific Notices for MBL-2 Antibody (14D12)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for MBL-2 Antibody (14D12)

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Protocols

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