MBP Antibody (CL2827) - BSA Free

Novus Biologicals | Catalog # NBP2-46631

Novus Biologicals
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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # CL2827

Format

BSA Free
View all MBP Primary Antibodies »

Product Specifications

Immunogen

This antibody was developed using a recombinant protein derived from P02686, with the exact immunogen sequence remaining proprietary.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for MBP Antibody (CL2827) - BSA Free

Western Blot: MBP Antibody (CL2827) [NBP2-46631]

Western Blot: MBP Antibody (CL2827) [NBP2-46631]

Western Blot: MBP Antibody (CL2827) [NBP2-46631] - Lane 1: Marker [kDa] 250, 130, 100, 70, 55, 35, 25, 15, 10. Lane 2: Mouse Cerebral Cortex tissue
Immunocytochemistry/ Immunofluorescence: MBP Antibody (CL2827) [NBP2-46631]

Immunocytochemistry/ Immunofluorescence: MBP Antibody (CL2827) [NBP2-46631]

Immunocytochemistry/Immunofluorescence: MBP Antibody (CL2827) [NBP2-46631] - Staining of A549 cells using the Anti-MBP monoclonal antibody, showing specific staining in the nucleoplasm in green. Microtubule- and nuclear probes are visualized in red and blue, respectively (where available).
Immunohistochemistry: MBP Antibody (CL2827) [NBP2-46631]

Immunohistochemistry: MBP Antibody (CL2827) [NBP2-46631]

Immunohistochemistry: MBP Antibody (CL2827) [NBP2-46631] - Staining of mouse striatum shows strong positivity in myelinated fibers.
Western Blot: MBP Antibody (CL2827) [NBP2-46631]

Western Blot: MBP Antibody (CL2827) [NBP2-46631]

Western Blot: MBP Antibody (CL2827) [NBP2-46631] - Lane 1: Marker [kDa] 250, 130, 100, 70, 55, 35, 25, 15, 10. Lane 2: Human Cerebral Cortex tissue
Immunohistochemistry: MBP Antibody (CL2827) [NBP2-46631]

Immunohistochemistry: MBP Antibody (CL2827) [NBP2-46631]

Immunohistochemistry: MBP Antibody (CL2827) [NBP2-46631] - Staining of rat striatum shows strong immunoreactivity in myelinated neural fibers.
Immunohistochemistry: MBP Antibody (CL2827) [NBP2-46631]

Immunohistochemistry: MBP Antibody (CL2827) [NBP2-46631]

Immunohistochemistry: MBP Antibody (CL2827) [NBP2-46631] - Staining of mouse cerebral cortex shows strong immunoreactivity in myelinated fibers.
Immunohistochemistry: MBP Antibody (CL2827) [NBP2-46631]

Immunohistochemistry: MBP Antibody (CL2827) [NBP2-46631]

Immunohistochemistry: MBP Antibody (CL2827) [NBP2-46631] - Staining of human cerebral cortex shows strong immunoreactivity in myelinated fibers.
Immunohistochemistry: MBP Antibody (CL2827) [NBP2-46631]

Immunohistochemistry: MBP Antibody (CL2827) [NBP2-46631]

Immunohistochemistry: MBP Antibody (CL2827) [NBP2-46631] - Staining of rat cerebral cortex shows strong immunoreactivity in myelinated fibers.

Applications for MBP Antibody (CL2827) - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

2-10 ug/ml

Immunohistochemistry

1:5000 - 1:10000

Immunohistochemistry-Paraffin

1:5000 - 1:10000

Western Blot

1 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. For ICC/IF, PFA/Triton X-100 is recommended for fixation/permeabilization.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: MBP

Myelin Basic Protein (MBP) functions in the nervous system in myelination and is expressed in oligodendrocytes following differentiation. There are numerous isoforms generated by differential splicing events and post-translational modifications that have specialized functions. These functions include participation in signaling pathways prior to myelination, myelination or the re-myelination process following neural injury.

Long Name

Myelin Basic Protein

Alternate Names

Hmbpr, mld, shi

Entrez Gene IDs

4155 (Human)

Gene Symbol

MBP

UniProt

P02686

Additional MBP Products

Product Documents for MBP Antibody (CL2827) - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for MBP Antibody (CL2827) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

Glial Lineage Markers

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Protocols

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