MBP Antibody (CL2829)

Novus Biologicals | Catalog # NBP3-52461

Recombinant Monoclonal Antibody
Novus Biologicals

Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Mouse IgG1 Clone # CL2829
View all MBP Primary Antibodies »

Product Specifications

Immunogen

This antibody was developed using a recombinant protein derived from P02686, with the exact immunogen sequence remaining proprietary.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for MBP Antibody (CL2829)

Immunohistochemistry-Paraffin: MBP Antibody (CL2829) [NBP3-52461]

Immunohistochemistry-Paraffin: MBP Antibody (CL2829) [NBP3-52461]

Staining of human cerebellum shows strong positivity in myelinated neural fibers.
Immunohistochemistry-Paraffin: MBP Antibody (CL2829) [NBP3-52461]

Immunohistochemistry-Paraffin: MBP Antibody (CL2829) [NBP3-52461]

Staining of human cerebral cortex shows strong positivity in myelinated neural fibers.
Immunohistochemistry-Paraffin: MBP Antibody (CL2829) [NBP3-52461]

Immunohistochemistry-Paraffin: MBP Antibody (CL2829) [NBP3-52461]

Staining of mouse cerebellum shows strong positivity in myelinated neural fibers.
Immunohistochemistry-Paraffin: MBP Antibody (CL2829) [NBP3-52461]

Immunohistochemistry-Paraffin: MBP Antibody (CL2829) [NBP3-52461]

Staining of mouse hippocampus shows strong positivity in myelinated neural fibers.
Immunohistochemistry-Paraffin: MBP Antibody (CL2829) [NBP3-52461]

Immunohistochemistry-Paraffin: MBP Antibody (CL2829) [NBP3-52461]

Staining of rat basal forebrain shows strong positivity in myelinated neural fibers.
Immunohistochemistry-Paraffin: MBP Antibody (CL2829) [NBP3-52461]

Immunohistochemistry-Paraffin: MBP Antibody (CL2829) [NBP3-52461]

Staining of rat cerebral cortex shows strong positivity in myelinated neural fibers.

Applications for MBP Antibody (CL2829)

Application
Recommended Usage

Immunohistochemistry

1:5000 - 1:10000

Immunohistochemistry-Paraffin

1:5000 - 1:10000

Western Blot

1 ug/ml
Application Notes
For IHC-Paraffin, we recommend using Heat-Induced Epitope Retrieval (HIER) with a pH level of 6. This optimized retrieval method ensures the best results for your experiments.

Formulation, Preparation, and Storage

Purification

Protein A purified

Reconstitution

Centrifuge the vial of lyophilized antibody at 12,000 x g for 20 seconds. Reconstitute by adding sterile, distilled water to achieve a final antibody concentration of 1mg/ml.

Formulation

Lyophilized from a 0.2 um filtered solution in PBS with Trehalose

Preservative

0.02% Sodium Azide

Concentration

LYOPH mg/ml

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: MBP

Myelin Basic Protein (MBP) functions in the nervous system in myelination and is expressed in oligodendrocytes following differentiation. There are numerous isoforms generated by differential splicing events and post-translational modifications that have specialized functions. These functions include participation in signaling pathways prior to myelination, myelination or the re-myelination process following neural injury.

Long Name

Myelin Basic Protein

Alternate Names

Hmbpr, mld, shi

Gene Symbol

MBP

Additional MBP Products

Product Documents for MBP Antibody (CL2829)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for MBP Antibody (CL2829)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

Glial Lineage Markers

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Protocols

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FAQs

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