MBTPS1 Antibody - BSA Free

Novus Biologicals | Catalog # NBP3-05161

Novus Biologicals
100% Guarantee

Key Product Details

Species Reactivity

Human, Mouse

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, ELISA, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all MBTPS1/SKI-1 Primary Antibodies »

Product Specifications

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 803-1052 of human MBTPS1 (NP_003782.1). ITQTFKDQGLEVLKQETAVVENVPILGLYQIPAEGGGRIVLYGDSNCLDDSHRQKDCFWLLDALLQYTSYGVTPPSLSHSGNRQRPPSGAGSVTPERMEGNHLHRYSKVLEAHLGDPKPRPLPACPRLSWAKPQPLNETAPSNLWKHQKLLSIDLDKVVLPNFRSNRPQVRPLSPGESGAWDIPGGIMPGRYNQEVGQTIPVFAFLGAMVVLAFFVVQINKAKSRPKRRKPRVKRPQLMQQVHPPKTPSV

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

118 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for MBTPS1 Antibody - BSA Free

Western Blot: MBTPS1 AntibodyAzide and BSA Free [NBP3-05161]

Western Blot: MBTPS1 AntibodyAzide and BSA Free [NBP3-05161]

Western Blot: MBTPS1 Antibody [NBP3-05161] - Analysis of extracts of various cell lines, using MBTPS1 antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit
MBTPS1 Antibody - Azide and BSA Free

Western Blot: MBTPS1 Antibody - Azide and BSA Free [MBTPS1] -

Western Blot: MBTPS1 Antibody - Azide and BSA Free [MBTPS1] - Western blot analysis of lysates from Rat lung using MBTPS1 Rabbit pAb at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25 ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 90s.
MBTPS1 Antibody - Azide and BSA Free

Immunohistochemistry: MBTPS1 Antibody - Azide and BSA Free [MBTPS1] -

Immunohistochemistry: MBTPS1 Antibody - Azide and BSA Free [MBTPS1] - Immunohistochemistry analysis of paraffin-embedded Human colon carcinoma tissue using MBTPS1 Rabbit pAb at a dilution of 1:100 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.
MBTPS1 Antibody - Azide and BSA Free

Immunohistochemistry: MBTPS1 Antibody - Azide and BSA Free [MBTPS1] -

Immunohistochemistry: MBTPS1 Antibody - Azide and BSA Free [MBTPS1] - Immunohistochemistry analysis of paraffin-embedded Human liver cancer tissue using MBTPS1 Rabbit pAb at a dilution of 1:100 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.
MBTPS1 Antibody - Azide and BSA Free

Immunocytochemistry/ Immunofluorescence: MBTPS1 Antibody - Azide and BSA Free [MBTPS1] -

Immunocytochemistry/ Immunofluorescence: MBTPS1 Antibody - Azide and BSA Free [MBTPS1] - Immunofluorescence analysis of U2OS cells using MBTPS1 Rabbit pAb. Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Applications for MBTPS1 Antibody - BSA Free

Application
Recommended Usage

ELISA

Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.

Immunocytochemistry/ Immunofluorescence

1:50 - 1:200

Immunohistochemistry

1:50 - 1:200

Immunohistochemistry-Paraffin

1:50 - 1:200

Western Blot

1:500-1:2000

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.3), 50% glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: MBTPS1/SKI-1

The encoded protein has a central role in the regulation of lipid metabolism in cells. It is a sterol-regulated subtilisin-like serine protease that cleaves ER membrane-bound sterol regulatory element-binding proteins (SREBPs), a reaction that initiates the two-step proteolytic process by which transcriptionally active fragments of SREBPs are released from the membrane for translocation to the nucleus. The gene product is an integral membrane ER protein, with the bulk located in the ER lumen. It is synthesized as an inactive preproprotein that is self-activated by an intramolecular cleavage that generates the mature protein. [provided by RefSeq]

Long Name

Membrane Bound Transcription Factor Peptidase, Site 1

Alternate Names

KIAA0091, PCSK8, S1P, SEDKF, SKI-1

Gene Symbol

MBTPS1

Additional MBTPS1/SKI-1 Products

Product Documents for MBTPS1 Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for MBTPS1 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including Methotrexate, which is known to the State of California to cause reproductive toxicity with developmental effects. For more information, go to www.P65Warnings.ca.gov

Related Research Areas

Serine Proteases and Regulators

Customer Reviews for MBTPS1 Antibody - BSA Free

There are currently no reviews for this product. Be the first to review MBTPS1 Antibody - BSA Free and earn rewards!

Have you used MBTPS1 Antibody - BSA Free?

Submit a review and receive an Amazon gift card!

$25/€18/£15/$25CAN/¥2500 Yen for a review with an image

$10/€7/£6/$10CAN/¥1110 Yen for a review without an image

Submit a review
Amazon Gift Card

Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs

No product specific FAQs exist for this product.

View all FAQs for Antibodies