Key Product Details

Validated by

Biological Validation

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunoblotting, Knockdown Validated

Cited:

Immunoblotting, Knockdown Validated

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all MCAF1 Primary Antibodies »

Product Specifications

Immunogen

A synthetic peptide that maps to a region between residues 25 and 100 of human MBD1-containing Chromatin Associated Factor using the numbering given in entry AAQ92978.1 (GeneID 55729)

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for MCAF1 Antibody - BSA Free

Western Blot: MCAF1 Antibody [NB100-438]

Western Blot: MCAF1 Antibody [NB100-438]

MCAF1-Antibody-Western-Blot-NB100-438-img0012.jpg
Immunohistochemistry: MCAF1 Antibody [NB100-438]

Immunohistochemistry: MCAF1 Antibody [NB100-438]

Immunohistochemistry: MCAF1 Antibody [NB100-438] - Sample: FFPE section of human breast carcinoma. Antibody: Affinity purified rabbit anti-MCAF used at a dilution of 1:1,000 (1ug/ml). Detection: DAB
Western Blot: MCAF1 Antibody [NB100-438]

Western Blot: MCAF1 Antibody [NB100-438]

Western Blot: MCAF1 Antibody [NB100-438] - Nuclear extract (20 ug) from HeLa cells. Antibody used at 0.2 and 0.1 ug/ml.
Western Blot: MCAF1 Antibody [NB100-438]

Western Blot: MCAF1 Antibody [NB100-438]

Western Blot: MCAF1 Antibody [NB100-438] - Detection of Human MCAF by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa and 293T cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-MCAF antibody NB100-438 used for WB at 0.05 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.
Immunohistochemistry-Paraffin: MCAF1 Antibody [NB100-438]

Immunohistochemistry-Paraffin: MCAF1 Antibody [NB100-438]

Immunohistochemistry-Paraffin: MCAF1 Antibody [NB100-438] - FFPE section of human small cell lung cancer. NB100-438 used at a dilution of 1:250. Detection: DAB staining using Immunohistochemistry Accessory Kit

Applications for MCAF1 Antibody - BSA Free

Application
Recommended Usage

Immunohistochemistry

1:10-1:500

Immunohistochemistry-Paraffin

1:10-1:500

Western Blot

1:5000-1:20000
Application Notes
Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections. Use in immunoblotting reported in scientific literature (PMID:31511512).

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris-Citrate/Phosphate (pH 7.0 - 8.0)

Format

BSA Free

Preservative

0.09% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: MCAF1

MBD1-containing chromatin-associated factor (MCAF) is a 1270-residue mediator in the DNA methylation cycle of a variety of genomic functions. This nuclear protein will bind to the C-terminal transcriptional repression domain of MBD1 to form a blocking complex. This molecule will then prevent transcription from methylated promoters in a histone deacetylation-independent action (1).

Alternate Names

activating transcription factor 7 interacting protein, activating transcription factor 7-interacting protein 1, ATF7-interacting protein, ATFa-associated modulator, ATF-interacting protein, ATF-IP, FLJ10139, FLJ10688, hAM, MBD1-containing chromatin associated factor, MCAF1AM, MCAFMBD1-containing chromatin-associated factor 1, MMCP 6, MMCP6, MMCP-6, P621

Entrez Gene IDs

55729 (Human)

Gene Symbol

ATF7IP

UniProt

Q6VMQ6 (Human)

Additional MCAF1 Products

Product Documents for MCAF1 Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for MCAF1 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for MCAF1 Antibody - BSA Free

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Protocols

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