Mcl-1 Antibody (8C6) - BSA Free

Novus Biologicals | Catalog # NB100-2194

Novus Biologicals
100% Guarantee

Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, ELISA, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 8C6

Format

BSA Free
View all Mcl-1 Primary Antibodies »

Product Specifications

Immunogen

Purified recombinant fragment of human Mcl-1 expressed in E. Coli.

Localization

Cell Membrane, Cytoplasmic, Nuclear

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

37 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Mcl-1 Antibody (8C6) - BSA Free

Western Blot: Mcl-1 Antibody (8C6)BSA Free [NB100-2194]

Western Blot: Mcl-1 Antibody (8C6)BSA Free [NB100-2194]

Western Blot: Mcl-1 Antibody (8C6D4B1) [NB100-2194] - Analysis using MCL1 mouse mAb against Hela (1), BCBL-1 (2), Jurkat (3) and HL60 (4) cell lysate.
Immunocytochemistry/ Immunofluorescence: Mcl-1 Antibody (8C6) - BSA Free [NB100-2194]

Immunocytochemistry/ Immunofluorescence: Mcl-1 Antibody (8C6) - BSA Free [NB100-2194]

Immunocytochemistry/Immunofluorescence: Mcl-1 Antibody (8C6D4B1) [NB100-2194] - Analysis of HepG2 cells using MCL1 mouse mAb (green). Red: Actin filaments have been labeled with DY-554 phalloidin. Blue: DRAQ5 fluorescent DNA dye.
Immunohistochemistry-Paraffin: Mcl-1 Antibody (8C6) - BSA Free [NB100-2194]

Immunohistochemistry-Paraffin: Mcl-1 Antibody (8C6) - BSA Free [NB100-2194]

Immunohistochemistry-Paraffin: Mcl-1 Antibody (8C6D4B1) [NB100-2194] - Analysis of human lymphnode tissues using MCL1 mouse mAb with DAB staining.

Applications for Mcl-1 Antibody (8C6) - BSA Free

Application
Recommended Usage

ELISA

1:10000

Immunocytochemistry/ Immunofluorescence

1:200 - 1:1000

Immunohistochemistry

1:200 - 1:1000

Immunohistochemistry-Paraffin

1:200 - 1:1000

Western Blot

1:500-1:2000

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS

Format

BSA Free

Preservative

0.03% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Mcl-1

Mcl-1 (Myeloid cell leukemia-1) is Bcl-2-related and was identified as an early-induction gene that increased in expression during the differentiation of human myeloblastic leukemia cell ML-1, or exposure to different DNA damaging agents. The level of Mcl-1 is decreased in peripheral B lymphocytes undergoing apoptosis following treatment with apoptotic stimuli such as TGF-alpha 1 and forskolin. Expression of Mcl-1 is able to delay apoptosis induced by over-expression of c-myc in CHO 5AHSmyc cells. In hematopoietic FDC-P1 cells, Mcl-1 interacts with another Bcl-2-related protein, Bax, and prolongs cell viability after treatment with different apoptotic reagents.This monoclonal antibody detected a 37kd MCL1 in BCBL-1 cell lysate.

Long Name

Myeloid Cell Leukemia Sequence 1

Alternate Names

BCL2L3, Mcl1

Entrez Gene IDs

4170 (Human)

Gene Symbol

MCL1

Additional Mcl-1 Products

Product Documents for Mcl-1 Antibody (8C6) - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for Mcl-1 Antibody (8C6) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customer Reviews for Mcl-1 Antibody (8C6) - BSA Free

There are currently no reviews for this product. Be the first to review Mcl-1 Antibody (8C6) - BSA Free and earn rewards!

Have you used Mcl-1 Antibody (8C6) - BSA Free?

Submit a review and receive an Amazon gift card!

$25/€18/£15/$25CAN/¥2500 Yen for a review with an image

$10/€7/£6/$10CAN/¥1110 Yen for a review without an image

Submit a review
Amazon Gift Card

Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs for Mcl-1 Antibody (8C6) - BSA Free

Showing  1 - 1 of 1 FAQ Showing All

  • Q: What is the concentration of NB100-2194?

    A: The concentration of NB100-2194 is 1 mg/ml.

Showing  1 - 1 of 1 FAQ Showing All
View all FAQs for Antibodies