Mcl-1 Antibody (SI16-04)

Western Blot: Mcl-1 Antibody (SI16-04) [NBP2-67400]

Western Blot: Mcl-1 Antibody (SI16-04) [NBP2-67400] - Western blot analysis of Mcl-1 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rab

Immunocytochemistry/ Immunofluorescence: Mcl-1 Antibody (SI16-04) [NBP2-67400]

Immunocytochemistry/Immunofluorescence: Mcl-1 Antibody (SI16-04) [NBP2-67400] - Staining MCL1 in BT-20 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunohistochemistry-Paraffin: Mcl-1 Antibody (SI16-04) [NBP2-67400]

Immunohistochemistry-Paraffin: Mcl-1 Antibody (SI16-04) [NBP2-67400] - Immunohistochemical analysis of paraffin-embedded mouse kidney tissue using anti-Mcl-1 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1606-14, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.