MCM2 Antibody (JE42-39)

Western Blot: MCM2 Antibody (JE42-39) [NBP3-32577]

Western Blot: MCM2 Antibody (JE42-39) [NBP3-32577] - Western blot analysis of MCM2 on different lysates with Rabbit anti-MCM2 antibody (NBP3-32577) at 1/1,000 dilution.

Lane 1: SW480 cell lysate (20 ug/Lane)
Lane 2: SH-SY5Y cell lysate (20 ug/Lane)
Lane 3: Raji cell lysate (20 ug/Lane)
Lane 4: HeLa cell lysate (20 ug/Lane)
Lane 5: HL-60 cell lysate (20 ug/Lane)
Lane 6: Mouse thymus tissue lysate (40 ug/Lane)
Lane 7: Jurkat cell lysate (20 ug/Lane)
Lane 8: C2C12 cell lysate (20 ug/Lane)
Lane 9: U-87 MG cell lysate (20 ug/Lane)

Predicted band size: 102 kDa
Observed band size: 130 kDa

Exposure time: 40 seconds;

4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32577) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.

Immunohistochemistry: MCM2 Antibody (JE42-39) [NBP3-32577]

Immunohistochemistry: MCM2 Antibody (JE42-39) [NBP3-32577] - Immunohistochemical analysis of paraffin-embedded human lymph nodes tissue with Rabbit anti-MCM2 antibody (NBP3-32577) at 1/1,000 dilution.

The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32577) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Immunocytochemistry/ Immunofluorescence: MCM2 Antibody (JE42-39) [NBP3-32577]

Immunocytochemistry/ Immunofluorescence: MCM2 Antibody (JE42-39) [NBP3-32577] - Immunocytochemistry analysis of HeLa cells labeling MCM2 with Rabbit anti-MCM2 antibody (NBP3-32577) at 1/250 dilution.

Cells were fixed in 100% precooled methanol for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-MCM2 antibody (NBP3-32577) at 1/250 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.

Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.