Key Product Details

Validated by

Biological Validation

Species Reactivity

Human, Mouse

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunoprecipitation

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all MCM3 Primary Antibodies »

Product Specifications

Immunogen

A 38-amino acid synthetic peptide, which represented a portion of human Minichromosome Maintenance Deficient 3 encoded in part by exons 10 and 11 (LocusLink ID 4172).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for MCM3 Antibody - BSA Free

Western Blot: MCM3 Antibody [NB100-289]

Western Blot: MCM3 Antibody [NB100-289]

Western Blot: MCM3 Antibody [NB100-289] - Detection of Human MCM3 by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa and 293T cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-MCM3 antibody NB100-289 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.
Immunohistochemistry: MCM3 Antibody [NB100-289]

Immunohistochemistry: MCM3 Antibody [NB100-289]

Immunohistochemistry: MCM3 Antibody [NB100-289] - Sample: FFPE section of human colon carcinoma. Antibody: Affinity purified rabbit anti-MCM3 used at a dilution of 1:1,000 (1ug/ml). Detection: DAB
Western Blot: MCM3 Antibody [NB100-289]

Western Blot: MCM3 Antibody [NB100-289]

Western Blot: MCM3 Antibody [NB100-289] - RIPA extract (50 ug) from HeLa cells. Antibody used at 0.2 ug/ml.
MCM3 Antibody

Western Blot: Rabbit Polyclonal MCM3 Antibody [NB100-289]

Western Blot: Rabbit Polyclonal MCM3 Antibody [NB100-289] - Whole cell lysates from MDA-MB-231 and SUM159 cells were loaded with 50 ug/lane. 10% SDS-PAGE. MCM3 Antibody (NB100-289) was used for primary antibody: 1:1000, 4℃, overnight. Image from a verified customer review.

Applications for MCM3 Antibody - BSA Free

Application
Recommended Usage

Immunohistochemistry

1:1000 to 1:5000

Immunohistochemistry-Paraffin

1:1000 to 1:5000

Immunoprecipitation

2-5 ug//mg lysate

Western Blot

1:2000-1:10000
Application Notes
Western blot and immunoprecipitation. Suggested working dilutions: Western Blot - 1: 2,000-1:10,000 Immunohistochemistry/Immunocytochemistry - ND Immunoprecipitation - 2 to 5 ug/mg lysate** *The investigator should determine the optimal working dilution for a specific application. **Use NB 100-288 (MCM2 antibody) to IP the MCM complex

Reviewed Applications

Read 1 review rated 5 using NB100-289 in the following applications:

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris-Citrate/Phosphate (pH 7.0 - 8.0)

Format

BSA Free

Preservative

0.09% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: MCM3

MCM3 is one of the highly conserved mini-chromosome maintenance proteins that are involved in the initiation of eukaryotic genome replication. The hexameric protein complex formed by MCM proteins is a key component of the pre-replication complex (pre_RC) and may be involved in the formation of replication forks and in the recruitment of other DNA replication related proteins. This protein is a subunit of the protein complex that consists of MCM2-7. It has been shown to interact directly with MCM5/CDC46. This protein also interacts with, and thus is acetlyated by MCM3AP, a chromatin-associated acetyltransferase. The acetylation of this protein inhibits the initiation of DNA replication and cell cycle progression.

Alternate Names

cervical cancer proto-oncogene 5, DNA polymerase alpha holoenzyme-associated protein P1, DNA replication factor MCM3, DNA replication licensing factor MCM3, EC 3.6.4.12, HCC5, hRlf beta subunit, MCM3 minichromosome maintenance deficient 3, MCM3 minichromosome maintenance deficient 3 (S. cerevisiae), MGC1157, minichromosome maintenance complex component 3, minichromosome maintenance deficient (S. cerevisiae) 3, minichromosome maintenance deficient 3, P1.h, p102, P1-MCM3, replication licensing factor, beta subunit, RLF subunit beta, RLFB

Entrez Gene IDs

4172 (Human)

Gene Symbol

MCM3

Additional MCM3 Products

Product Documents for MCM3 Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for MCM3 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for MCM3 Antibody - BSA Free

Customer Reviews for MCM3 Antibody - BSA Free (1)

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Customer Images

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  • MCM3 Antibody
    Name: Yongkang Yang
    Application: Western Blot
    Sample Tested: Human MDA-MB-231 cells and SUM-159PT
    Species: Human
    Verified Customer | Posted 11/28/2024
    Western Blot: whole cell lysates from MDA-MB-231 and SUM159 cells were loaded with 50 ug/lane. 10% SDS-PAGE. MCM3 Antibody (NB100-289) was used for primary antibody: 1:1000, 4℃, overnight.
    MCM3 Antibody - BSA Free NB100-289

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