MCM7 Antibody - BSA Free

Novus Biologicals | Catalog # NBP1-85721

Novus Biologicals
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Key Product Details

Validated by

Orthogonal Validation

Species Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all MCM7 Primary Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: QSPTFMPLIMCPSQECQTNRSGGRLYLQTRGSRFIKFQEMKMQEHSDQVPVGNIPRSITVLVEGENTRIAQPGDHVSVTGIFLPILRTGFRQVVQGLLSETYLEAHRIVKMNKSEDDESGAGE

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for MCM7 Antibody - BSA Free

Immunocytochemistry/ Immunofluorescence: MCM7 Antibody [NBP1-85721]

Immunocytochemistry/ Immunofluorescence: MCM7 Antibody [NBP1-85721]

Immunocytochemistry/Immunofluorescence: MCM7 Antibody [NBP1-85721] - Immunofluorescent staining of human cell line MCF7 shows localization to nucleoplasm. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: MCM7 Antibody [NBP1-85721]

Immunohistochemistry-Paraffin: MCM7 Antibody [NBP1-85721]

Immunohistochemistry-Paraffin: MCM7 Antibody [NBP1-85721] - Staining in human appendix and pancreas tissues using anti-MCM7 antibody. Corresponding MCM7 RNA-seq data are presented for the same tissues.
Immunohistochemistry-Paraffin: MCM7 Antibody [NBP1-85721]

Immunohistochemistry-Paraffin: MCM7 Antibody [NBP1-85721]

Immunohistochemistry-Paraffin: MCM7 Antibody [NBP1-85721] - Staining of human appendix shows high expression.
Immunohistochemistry-Paraffin: MCM7 Antibody [NBP1-85721]

Immunohistochemistry-Paraffin: MCM7 Antibody [NBP1-85721]

Immunohistochemistry-Paraffin: MCM7 Antibody [NBP1-85721] - Staining of human pancreas shows low expression as expected.
MCM7 Antibody - BSA Free Western Blot: MCM7 Antibody - BSA Free [NBP1-85721]

Western Blot: MCM7 Antibody - BSA Free [NBP1-85721]

Analysis in mouse cell line NIH-3T3 and rat cell line NBT-II.
MCM7 Antibody - BSA Free Western Blot: MCM7 Antibody - BSA Free [NBP1-85721]

Western Blot: MCM7 Antibody - BSA Free [NBP1-85721]

Analysis in human cell line MCF-7.

Applications for MCM7 Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:200 - 1:500

Immunohistochemistry-Paraffin

1:200 - 1:500

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. Immunocytochemistry/Immunofluorescence Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: MCM7

The minichromosome maintenance (MCM7) helicase complex functions to initiate and elongate replication forks. Cell cycle checkpoint signaling pathways regulate DNA replication to maintain genomic stability (1). MCM7 is a putative human homologue of yeast CDC47 and a member of the MCM protein family, which has been implicated in the regulatory machinery causing DNA to replicate only once in the S phase. Findings indicate that MCM7 protein together with other MCM proteins participates in the regulation of mammalian DNA replication (2). In HeLa cells, depletion of MCM7 with small-interfering RNA suppressed ultraviolet (UV) light- or aphidicolin-induced hChk1 phosphorylation, and abolished UV-induced S-phase checkpoint activation. These results demonstrate that MCM7 plays a direct role in the transmission of DNA damage signals from active replication forks to the S-phase checkpoint machinery in human cells (3).

Long Name

Minichromosome Maintenance Protein 7

Alternate Names

CDC47, MCM2, P1CDC47, P85MCM, PNAS146, PPP1R104, Protein Phosphatase 1

Gene Symbol

MCM7

Additional MCM7 Products

Product Documents for MCM7 Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for MCM7 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

Cell Cycle

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Protocols

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FAQs

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