MD-2 Antibody (1A2E3) - BSA Free

Novus Biologicals | Catalog # NBP1-75513

Novus Biologicals
100% Guarantee

Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, ELISA, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 1A2E3

Format

BSA Free
View all MD2 Primary Antibodies »

Product Specifications

Immunogen

Antibody was raised against a recombinant protein corresponding to amino acids 2 to 160 of human MD-2.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for MD-2 Antibody (1A2E3) - BSA Free

Western Blot: MD-2 Antibody (1A2E3)BSA Free [NBP1-75513]

Western Blot: MD-2 Antibody (1A2E3)BSA Free [NBP1-75513]

Western Blot: MD-2 Antibody (1A2E3) [NBP1-75513] - Analysis of (A) 25 and (B) 125 ng of MD-2 recombinant protein with MD-2 antibody at 1 ug/ml.
MD-2 Antibody (1A2E3) - BSA Free

Immunocytochemistry/ Immunofluorescence: MD-2 Antibody (1A2E3) - BSA Free [NBP1-75513] -

Immunocytochemistry/ Immunofluorescence: MD-2 Antibody (1A2E3) - BSA Free [NBP1-75513] - Immunofluorescence of MD-2 in human spleen with MD-2 Antibody at 20 u/ml.

Applications for MD-2 Antibody (1A2E3) - BSA Free

Application
Recommended Usage

ELISA

1:100-1:2000

Immunohistochemistry

2 ug/ml

Immunohistochemistry-Paraffin

2 ug/ml

Western Blot

1 ug/ml

Reviewed Applications

Read 1 review rated 5 using NBP1-75513 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: MD2

MD-2 is a member of the Toll/interleukin-1 receptor (TIR) family, a group of proteins that include the Toll-like receptors (TLRs). TLRs are signaling molecules that recognize different pathogen-associated molecular patterns (PAMPs) and serve as an important link between the innate and adaptive immune responses. TLR4, the major signaling receptor for lipopolysaccharide (LPS), requires the binding of MD-2 to its extracellular region for maximal response to LPS. The specificity of this response is determined by the species of MD-2; e.g., human MD-2 transfected into mouse cells can cause mouse TLR4 to react to LPS analogs that are normally antagonistic to human but not mouse TLR4.

Long Name

Myeloid Differentiation Protein 2/Lymphocyte Antigen 96

Alternate Names

ESOP-1, LY96, MD-2

Gene Symbol

LY96

UniProt

NP_056179 (Human)

Additional MD2 Products

Product Documents for MD-2 Antibody (1A2E3) - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for MD-2 Antibody (1A2E3) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

Monocyte Markers
Toll-Like Receptors

Citations for MD-2 Antibody (1A2E3) - BSA Free

Customer Reviews for MD-2 Antibody (1A2E3) - BSA Free (1)

5 out of 5
1 Customer Rating
5 Stars
100%
4 Stars
0%
3 Stars
0%
2 Stars
0%
1 Stars
0%

Have you used MD-2 Antibody (1A2E3) - BSA Free?

Submit a review and receive an Amazon gift card!

$25/€18/£15/$25CAN/¥2500 Yen for a review with an image

$10/€7/£6/$10CAN/¥1110 Yen for a review without an image

Submit a review
Amazon Gift Card

Customer Images

Showing  1 - 1 of 1 review Showing All
Filter By:
Clear Filters X
  • MD-2 Antibody (1A2E3)
    Name: Anonymous
    Application: Western Blot
    Sample Tested: Spleen tissue
    Species: Mouse
    Verified Customer | Posted 09/08/2021
    Spleen tissue
    MD-2 Antibody (1A2E3) - BSA Free NBP1-75513

There are no reviews that match your criteria.

Showing  1 - 1 of 1 review Showing All

Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs for MD-2 Antibody (1A2E3) - BSA Free

Showing  1 - 1 of 1 FAQ Showing All

  • Q: Have NBP1-75513 and NBP1-76709 been tested using immunoprecipitation techniques?

    A:

    Unfortunately neither of these antibodies have been validated for IP. However, we do supply alternative products; NB100-56700 for instance is another LY86 antibody which has been tested for IP. Unfortunately none of our MD2 antibodies are validated for IP. If you would like to test any of these antibodies for an untested application/species, I recommend that you take advantage of our Innovator's Reward program.

Showing  1 - 1 of 1 FAQ Showing All
View all FAQs for Antibodies