MDG1 Antibody

Novus Biologicals | Catalog # NBP1-78785

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human, Mouse

Cited:

Human

Predicted:

Bovine (100%), Canine (100%), Porcine (100%), Rat (100%). Backed by our 100% Guarantee.

Applications

Validated:

Western Blot, Peptide ELISA, Flow Cytometry, Immunocytochemistry/ Immunofluorescence

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all MDG1 Primary Antibodies »

Product Specifications

Immunogen

This MDG1 Antibody was developed against a peptide with sequence C-PDAEAKFREIAEAYE corresponding to internal region according to NP_036460.1.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for MDG1 Antibody

Western Blot: MDG1 Antibody [NBP1-78785]

Western Blot: MDG1 Antibody [NBP1-78785]

Western Blot: MDG1 Antibody [NBP1-78785] - Staining of Human Liver (A), Kidney (B) and -Mouse Liver (C) lysate (35 ug protein in RIPA buffer) with antibody at 1 ug/mL. Detected by chemiluminescence. Approx 26-27 kDa band observed in Human Liver and Kidney lysates and approx. 28 kDa in Mouse Liver lysates (calculated MW of 25.5 kDa according to Human NP_036460.1 and 25.6 kDa according to Mouse NP_038788.2).
Immunocytochemistry/ Immunofluorescence: MDG1 Antibody [NBP1-78785]

Immunocytochemistry/ Immunofluorescence: MDG1 Antibody [NBP1-78785]

Immunocytochemistry/Immunofluorescence: MDG1 Antibody [NBP1-78785] - Immunofluorescence analysis of paraformaldehyde fixed U2OS cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL), showing endoplasmic reticulum/cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL).
Flow Cytometry: MDG1 Antibody [NBP1-78785]

Flow Cytometry: MDG1 Antibody [NBP1-78785]

Flow Cytometry: MDG1 Antibody [NBP1-78785] - Flow cytometric analysis of paraformaldehyde fixed A431 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (1 ug/mL). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.
Immunocytochemistry/ Immunofluorescence: MDG1 Antibody [NBP1-78785]

Immunocytochemistry/ Immunofluorescence: MDG1 Antibody [NBP1-78785]

Immunocytochemistry/Immunofluorescence: MDG1 Antibody [NBP1-78785] - Immunofluorescence analysis of paraformaldehyde fixed A431 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL), showing endoplasmic reticulum and cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL).

Applications for MDG1 Antibody

Application
Recommended Usage

Flow Cytometry

10 ug/mL

Immunocytochemistry/ Immunofluorescence

10 ug/mL

Peptide ELISA

Detection limit 1:16000

Western Blot

1 - 3 ug/mL

Flow Cytometry Panel Builder

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA

Preservative

0.02% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: MDG1

MDG1 acts as a co-chaperone with an Hsp70 protein

Alternate Names

DKFZp564F1862, DnaJ (Hsp40) homolog, subfamily B, member 9, dnaJ homolog subfamily B member 9, endoplasmic reticulum DnaJ homolog 4, ERdj4, MDG-1, MDG1MSTP049, Microvascular endothelial differentiation gene 1 protein, MST049

Entrez Gene IDs

4189 (Human)

Gene Symbol

DNAJB9

UniProt

NP_036460.1 (Human)

Additional MDG1 Products

Product Documents for MDG1 Antibody

Certificate of Analysis

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Product Specific Notices for MDG1 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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