MDM2/HDM2 Antibody (HDM2-323) - BSA Free

Novus Biologicals | Catalog # NB600-1312

Novus Biologicals
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Key Product Details

Species Reactivity

Human, Mouse

Applications

Western Blot, ELISA

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2A Clone # HDM2-323

Format

BSA Free
View all MDM2/HDM2 Primary Antibodies »

Product Specifications

Immunogen

Synthetic peptide conjugated to KLH, corresponding to amino acids 389-402 of Human MDM2.

Epitope

The epitope recognized by the antibody is localized in the C-terminal region of MDM2 (amino acids 389-402 of human MDM2).

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.

Localization

Nuclear and cytoplasmic. Expressed predominantly in the nucleoplasm. Interaction with ARF (P14) results in the localisation of both proteins to the nucleus. The nucleolar localisation signals in both ARF and MDM2 may be necessary to allow efficient nucleo

Specificity

MDM2 (HDM2-323)

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Scientific Data Images for MDM2/HDM2 Antibody (HDM2-323) - BSA Free

Western Blot: MDM2/HDM2 Antibody (HDM2-323)BSA Free [NB600-1312]

Western Blot: MDM2/HDM2 Antibody (HDM2-323)BSA Free [NB600-1312]

Western Blot: MDM2/HDM2 Antibody (HDM2-323) [NB600-1312] - Whole extracts of HEK-293 cells overexpressing human (lane 1) or mouse (lane 2) MDM2 were separated on SDS-PAGE and probed with 2 ug/mL Monoclonal Anti-MDM2 Clone: HDM2-323. The antibody was developed using Goat Anti-Mouse IgG-Peroxidase and a chemiluminescent substrate.

Applications for MDM2/HDM2 Antibody (HDM2-323) - BSA Free

Application
Recommended Usage

ELISA

1:100-1:2000

Western Blot

1-2 ug/ml
Application Notes
WB: Detects a band of approximately 90 kDa. Not tested in other applications.Optimal dilutions/concentrations should be determined by the end user.

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

10mM PBS (pH 7.4)

Format

BSA Free

Preservative

0.09% Sodium Azide

Concentration

2.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: MDM2/HDM2

MDM2 is an E3 ubiquitin ligase that recognizes the N-terminal trans-activation domain (TAD) of the p53 transcription factor. MDM2 is a p53 responsive gene

Long Name

Double Minute 2 Protein

Alternate Names

HDM2

Entrez Gene IDs

4193 (Human); 17246 (Mouse)

Gene Symbol

MDM2

Additional MDM2/HDM2 Products

Product Documents for MDM2/HDM2 Antibody (HDM2-323) - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for MDM2/HDM2 Antibody (HDM2-323) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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