MDMX Antibody (2D10F4)

Novus Biologicals | Catalog # NBP1-28862

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, ELISA, Immunocytochemistry/ Immunofluorescence

Cited:

Western Blot, IF/IHC

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 2D10F4
View all MDM4/MDMX Primary Antibodies »

Product Specifications

Immunogen

Purified recombinant fragment of human MDMX expressed in E. Coli.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

55 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for MDMX Antibody (2D10F4)

Western Blot: MDMX Antibody (2D10F4)BSA Free [NBP1-28862]

Western Blot: MDMX Antibody (2D10F4)BSA Free [NBP1-28862]

Western Blot: MDMX Antibody (2D10F4) [NBP1-28862] - Analysis using MDM4 mouse mAb against Hela (1), A549 (2) and A431 (3) cell lysate.
Immunocytochemistry/ Immunofluorescence: MDMX Antibody (2D10F4) - BSA Free [NBP1-28862]

Immunocytochemistry/ Immunofluorescence: MDMX Antibody (2D10F4) - BSA Free [NBP1-28862]

Immunocytochemistry/Immunofluorescence: MDMX Antibody (2D10F4) [NBP1-28862] - Analysis of Hela (left) and L-02 (right) cells using MDM4 mouse mAb (green). Red: Actin filaments have been labeled with DY-554 phalloidin.
Immunohistochemistry-Paraffin: MDMX Antibody (2D10F4) - BSA Free [NBP1-28862]

Immunohistochemistry-Paraffin: MDMX Antibody (2D10F4) - BSA Free [NBP1-28862]

Immunohistochemistry-Paraffin: MDMX Antibody (2D10F4) [NBP1-28862] - Analysis of human Tonsil tissues using MDM4 mouse mAb
Immunohistochemistry-Paraffin: MDMX Antibody (2D10F4) - BSA Free [NBP1-28862]

Immunohistochemistry-Paraffin: MDMX Antibody (2D10F4) - BSA Free [NBP1-28862]

Immunohistochemistry-Paraffin: MDMX Antibody (2D10F4) [NBP1-28862] - Analysis of human cerebra (left) and lung carcinoma (right) tissues, showing nuclear localization with DAB staining using MDM4 mouse mAb.

Applications for MDMX Antibody (2D10F4)

Application
Recommended Usage

ELISA

1:10000

Immunocytochemistry/ Immunofluorescence

1:200 - 1:1000

Immunohistochemistry

1:10 - 1:500

Immunohistochemistry-Paraffin

1:200 - 1:1000

Western Blot

1:500 - 1:2000

Formulation, Preparation, and Storage

Purification

Ascites

Formulation

Ascites

Preservative

0.03% Sodium Azide

Concentration

This product is unpurified. The exact concentration of antibody is not quantifiable.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: MDM4/MDMX

Mdm4 p53 binding protein homolog (mouse). The human MDM4 gene, which plays a role in apoptosis, encodes a 490-amino acid protein containing a RING finger domain and a putative nuclear localization signal. The MDM4 putative nuclear localization signal, which all Mdm proteins contain, is located in the C-terminal region of the protein. The mRNA is expressed at a high level in thymus and at lower levels in all other tissues tested. MDM4 protein produced by in vitro translation interacts with p53 via a binding domain located in the N-terminal region of the MDM4 protein. MDM4 shows significant structural similarity to p53-binding protein MDM2

Long Name

Double Minute 4 Protein

Alternate Names

MDM4 Regulator of P53, MDMX, MRP1

Entrez Gene IDs

4194 (Human)

Gene Symbol

MDM4

UniProt

O15151 (Human)

Additional MDM4/MDMX Products

Product Documents for MDMX Antibody (2D10F4)

Certificate of Analysis

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Product Specific Notices for MDMX Antibody (2D10F4)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

p53 Pathway

Citations for MDMX Antibody (2D10F4)

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Protocols

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