MDR1/ABCB1 Antibody - BSA Free

Novus Biologicals | Catalog # NB100-80870

Novus Biologicals
100% Guarantee

Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat, Canine, Monkey

Cited:

Human, Mouse

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, ELISA

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all MDR1/ABCB1 Primary Antibodies »

Product Specifications

Immunogen

Synthetic peptide from human MDR1/ABCB1.

Epitope

Internal region near amino acids 255-280

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for MDR1/ABCB1 Antibody - BSA Free

Immunohistochemistry-Paraffin: MDR1/ABCB1 Antibody - BSA Free [NB100-80870]

Immunohistochemistry-Paraffin: MDR1/ABCB1 Antibody - BSA Free [NB100-80870]

Immunohistochemistry-Paraffin: MDR1/ABCB1 Antibody [NB100-80870] - Staining of human kidney tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
MDR1/ABCB1 Antibody - BSA Free

Western Blot: MDR1/ABCB1 Antibody - BSA Free [NB100-80870] -

Acetylated androstano-arylpyrimidines do not suppress the efflux activity of multidrug-resistant cells by attenuating the expression of ABC transporters. (A) Relative mRNA levels of selected ABC transporters in Colo 320 cells treated with acetylated androstano-arylpyrimidines 10a, 10d, 10e, 10f, and 10g (in 20 μM concentration for 24 h) determined by RT-qPCR measurements and subsequent analyses by the delta delta Ct method using GAPDH as the reference gene. For primer sequences and concentrations, please refer to Supplementary Material Table S3. (B) Representative Western blot images and densitometric analysis of ABCB1 protein levels in Colo 320 cells following exposure to androstano-arylpyrimidine acetates (treatment with steroid compounds at 20 μM for 24 h) or Tunicamycin (600 nM, 24 h). The equal loading of protein samples was verified by probing the membrane with an anti-actin antibody. C = Control, Tun = Tunicamycin. In both panels, bar graphs represent mean +/- SD values. Fisher’s LSD test, *: p < 0.05, **: p < 0.01, ***: p < 0.001, ****: p < 0.0001 ns: non-significant. Image collected and cropped by CiteAb from the following open publication (https://www.mdpi.com/1999-4923/15/2/584), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Applications for MDR1/ABCB1 Antibody - BSA Free

Application
Recommended Usage

ELISA

1:10000 - 1:50000

Immunohistochemistry

5 ug/ml

Immunohistochemistry-Paraffin

5 ug/ml

Western Blot

1:200 - 1:2000
Application Notes
IHC: NB100-80870 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity..

Reviewed Applications

Read 1 review rated 5 using NB100-80870 in the following applications:

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

20mM Potassium Phosphate (pH 7.2) and 0.15M NaCl

Format

BSA Free

Preservative

0.01% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Keep as concentrated solution. Aliquot and store at -20C or below. Avoid multiple freeze-thaw cycles.

Background: MDR1/ABCB1

P Glycoprotein, the product of the MDR1 gene, is expressed in distinct non-malignant cells, typically cells with secretory and excretory functions. It is assumed to function as an ATP-dependent drug efflux pump with broad substrate specificity. The highest expression of P Glycoprotein has been observed in kidney (proximal tubules), liver (bile canaliculi), adrenal gland and intestine, suggesting that the primary role of P Glycoprotein is in the normal secretion of physiological metabolites and ingested chemicals into bile, urine and the lumen of the intestinal tract. Elevated levels of P Glycoprotein have also been reported in multidrug-resistant cell lines and in colon, endometrial, ovarian, and breast tumors, as well as in sarcomas and leukemias / lymphomas.

Long Name

ATP-binding Cassette, Sub-family B (MDR/TAP), Member 1B

Alternate Names

ABC20, ABCB1, ABCB1B, CD243, CLCS, GP170, IBD13, PGY1

Entrez Gene IDs

5243 (Human); 18671 (Mouse); 24646 (Rat)

Gene Symbol

ABCB1

UniProt

P08183 (Human)

Additional MDR1/ABCB1 Products

Product Documents for MDR1/ABCB1 Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for MDR1/ABCB1 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for MDR1/ABCB1 Antibody - BSA Free

Customer Reviews for MDR1/ABCB1 Antibody - BSA Free (1)

5 out of 5
1 Customer Rating
5 Stars
100%
4 Stars
0%
3 Stars
0%
2 Stars
0%
1 Stars
0%

Have you used MDR1/ABCB1 Antibody - BSA Free?

Submit a review and receive an Amazon gift card!

$25/€18/£15/$25CAN/¥2500 Yen for a review with an image

$10/€7/£6/$10CAN/¥1110 Yen for a review without an image

Submit a review
Amazon Gift Card

Customer Images

Showing  1 - 1 of 1 review Showing All
Filter By:
Clear Filters X
  • Name: Ewa Kaniewska
    Application: Western Blot
    Sample Tested:
    Species: Human
    Verified Customer | Posted 05/24/2014
    MDR1/ABCB1 Antibody - BSA Free NB100-80870

There are no reviews that match your criteria.

Showing  1 - 1 of 1 review Showing All

Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs for MDR1/ABCB1 Antibody - BSA Free

Showing  1 - 1 of 1 FAQ Showing All

  • Q: I'm wondering what advice you have in regards to storing antibodies in glycerol solution to prevent freeze thaw damage. I recently purchased an MDR1 antibody, rabbit host and polyclonal. The concentration is 1mg/ml.

    A: Glycerol can indeed be added to the buffer for long term storage to help prevent freeze thaw cycle damage. Our lab recommends adding 30%.

Showing  1 - 1 of 1 FAQ Showing All
View all FAQs for Antibodies