ME1 Antibody (3H5) - Azide and BSA Free

Novus Biologicals | Catalog # H00004199-M03

Novus Biologicals
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Key Product Details

Species Reactivity

Human, Mouse

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, ELISA, Sandwich ELISA, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # 3H5

Format

Azide and BSA Free
View all ME1 Primary Antibodies »

Product Specifications

Immunogen

ME1 (AAH25246, 1 a.a. ~ 572 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MEPEAPRRRHTHQRGYLLTRNPHLNKDLAFTLEERQQLNIHGLLPPSFNSQEIQVLRVVKNFEHLNSDFDRYLLLMDLQDRNEKLFYRVLTSDIEKFMPIVYTPTVGLACQQYSLVFRKPRGLFITIHDRGHIASVLNAWPEDVIKAIVVTDGERILGLGDLGCNGMGIPVGKLALYTACGGMNPQECLPVILDVGTENEELLKDPLYIGLRQRRVRGSEYDDFLDEFMEAVSSKYGMNCLIQFEDFANVNAFRLLNKYRNQYCTFNDDIQGTASVAVAGLLAALRITKNKLSDQTILFQGAGEAALGIAHLIVMALEKEGLPKEKAIKKIWLVDSKGLIVKGRASLTQEKEKFAHEHEEMKNLEAIVQEIKPTALIGVAAIGGAFSEQILKDMAAFNERPIIFALSNPTSKAECSAEQCYKITKGRAIFASGSPFDPVTLPNGQTLYPGQGNNSYVFPGVALGVVACGLRQITDNIFLTTAEVIAQQVSDKHLEEGRLYPPLNTIRDVSLKIAEKIVKDAYQEKTATVYPEPQNKEAFVRSQMYSTDYDQILPDCYSWPEEVQKIQTKVDQ

Specificity

ME1 - malic enzyme 1, NADP(+)-dependent, cytosolic

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Description

Quality control test: Antibody Reactive Against Recombinant Protein.

Scientific Data Images for ME1 Antibody (3H5) - Azide and BSA Free

Western Blot: ME1 Antibody (3H5) [H00004199-M03]

Western Blot: ME1 Antibody (3H5) [H00004199-M03]

Western Blot: ME1 Antibody (3H5) [H00004199-M03] - Analysis of ME1 expression in transfected 293T cell line by ME1 monoclonal antibody (M03), clone 3H5. Lane 1: ME1 transfected lysatE (64.1 KDa). Lane 2: Non-transfected lysate.
Immunohistochemistry-Paraffin: ME1 Antibody (3H5) [H00004199-M03]

Immunohistochemistry-Paraffin: ME1 Antibody (3H5) [H00004199-M03]

Immunohistochemistry-Paraffin: ME1 Antibody (3H5) [H00004199-M03] - Analysis of monoclonal antibody to ME1 on formalin-fixed paraffin-embedded human tonsil. Antibody concentration 0.5 ug/ml
Western Blot: ME1 Antibody (3H5) [H00004199-M03]

Western Blot: ME1 Antibody (3H5) [H00004199-M03]

Western Blot: ME1 Antibody (3H5) [H00004199-M03] - Analysis of ME1 expression in HepG2 (Cat # L019V1).
Sandwich ELISA: ME1 Antibody (3H5) [H00004199-M03] - Detection limit for recombinant GST tagged ME1 is approximately 1ng/ml as a capture antibody.

Applications for ME1 Antibody (3H5) - Azide and BSA Free

Application
Recommended Usage

Western Blot

1:500
Application Notes
Antibody reactivity against cell lysate and recombinant protein for WB. It has also been used for IHC-P and ELISA.

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: ME1

This gene encodes a cytosolic, NADP-dependent enzyme that generates NADPH for fatty acid biosynthesis. The activity of this enzyme, the reversible oxidative decarboxylation of malate, links the glycolytic and citric acid cycles. The regulation of expression for this gene is complex. Increased expression can result from elevated levels of thyroid hormones or by higher proportions of carbohydrates in the diet.

Alternate Names

EC 1.1.1, EC 1.1.1.40, HUMNDME, malate dehydrogenase, Malic enzyme 1, malic enzyme 1, NADP(+)-dependent, cytosolic, malic enzyme 1, soluble, Malic enzyme, cytoplasmic, MES, NADP-dependent malic enzyme, NADP-ME, pyruvic-malic carboxylase

Entrez Gene IDs

4199 (Human)

Gene Symbol

ME1

OMIM

154250 (Human)

UniProt

AAH25246 (Human)

Additional ME1 Products

Product Documents for ME1 Antibody (3H5) - Azide and BSA Free

Certificate of Analysis

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Product Specific Notices for ME1 Antibody (3H5) - Azide and BSA Free

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for ME1 Antibody (3H5) - Azide and BSA Free

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Protocols

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FAQs

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