ME1 Antibody (979) - Azide and BSA Free

Novus Biologicals | Catalog # NBP2-43710

Novus Biologicals
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Key Product Details

Validated by

Knockout/Knockdown

Species Reactivity

Validated:

Human, Mouse

Predicted:

Bovine (91%), Rat (91%), Sheep (92%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 979

Format

Azide and BSA Free
View all ME1 Primary Antibodies »

Product Specifications

Immunogen

Recombinant protein encompassing a sequence within the center region of human ME1. The exact sequence is proprietary.

Reactivity Notes

Chicken (80%).

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

64 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for ME1 Antibody (979) - Azide and BSA Free

Immunohistochemistry-Paraffin: ME1 Antibody (979) [NBP2-43710]

Immunohistochemistry-Paraffin: ME1 Antibody (979) [NBP2-43710]

Immunohistochemistry-Paraffin: ME1 Antibody (979) [NBP2-43710] - Paraffin-embedded mouse testis. ME1 antibody (979) diluted at 1:250.
Western Blot: ME1 Antibody (979) [NBP2-43710]

Western Blot: ME1 Antibody (979) [NBP2-43710]

Western Blot: ME1 Antibody (979) [NBP2-43710] - Analysis of various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with ME1 antibody diluted at a dilution of 1:1000.
ME1 Antibody (979) - Azide and BSA Free

Immunocytochemistry/ Immunofluorescence: ME1 Antibody (979) [NBP2-43710] -

ME1 antibody [GT979] detects ME1 protein at cytoplasm by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: ME1 protein stained by ME1 antibody [GT979] (NBP2-43710) diluted at 1:400.
Blue: Hoechst 33342 staining.
ME1 Antibody (979) - Azide and BSA Free

Western Blot: ME1 Antibody (979) [NBP2-43710] -

Non-transfected (-) and transfected (+) HeLa whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with ME1 antibody [GT979] (NBP2-43710) diluted at 1:1000.

Applications for ME1 Antibody (979) - Azide and BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100-1:1000

Immunohistochemistry

1:100-1:1000

Immunohistochemistry-Paraffin

1:100-1:1000

Western Blot

1:500-1:3000

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: ME1

ME1 encodes a cytosolic, NADP-dependent enzyme that generates NADPH for fatty acid biosynthesis. The activity of this enzyme, the reversible oxidative decarboxylation of malate, links the glycolytic and citric acid cycles. The regulation of expression for this gene is complex. Increased expression can result from elevated levels of thyroid hormones or by higher proportions of carbohydrates in the diet.

Alternate Names

EC 1.1.1, EC 1.1.1.40, HUMNDME, malate dehydrogenase, Malic enzyme 1, malic enzyme 1, NADP(+)-dependent, cytosolic, malic enzyme 1, soluble, Malic enzyme, cytoplasmic, MES, NADP-dependent malic enzyme, NADP-ME, pyruvic-malic carboxylase

Gene Symbol

ME1

Additional ME1 Products

Product Documents for ME1 Antibody (979) - Azide and BSA Free

Certificate of Analysis

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Product Specific Notices for ME1 Antibody (979) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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