Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, ELISA, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
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Product Specifications

Immunogen

ME2 antibody was raised against a 17 amino acid peptide near the center of human ME2. The immunogen is located within amino acids 340 - 390 of ME2. Amino Acid Squence: KGRKAKIDSYQEPFTHS

Specificity

ME2 antibody is human specific. At least two isoforms of ME2 are known to exist; this antibody will detect both isoforms. ME2 antibody is predicted not to cross-react with ME1.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

60 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for ME2 Antibody - BSA Free

Western Blot: ME2 AntibodyBSA Free [NBP2-82105]

Western Blot: ME2 AntibodyBSA Free [NBP2-82105]

Western Blot: ME2 Antibody [NBP2-82105] - Western blot analysis of ME2 in human spleen tissue lysate with ME2 antibody at (A) 1 and (B) 2ug/ml.
ME2 Antibody - BSA Free

Immunohistochemistry: ME2 Antibody - BSA Free [NBP2-82105] -

Immunohistochemistry: ME2 Antibody - BSA Free [NBP2-82105] - Immunohistochemistry of ME2 in human spleen tissue with ME2 antibody at 5 u/mL.
ME2 Antibody - BSA Free

Immunocytochemistry/ Immunofluorescence: ME2 Antibody - BSA Free [NBP2-82105] -

Immunocytochemistry/ Immunofluorescence: ME2 Antibody - BSA Free [NBP2-82105] - Immunofluorescence of ME2 in human spleen tissue with ME2 antibody at 20 ug/mL.

Applications for ME2 Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

20 ug/ml

Immunohistochemistry

5 ug/ml

Western Blot

1-2 ug/ml

Formulation, Preparation, and Storage

Purification

Peptide affinity purified

Formulation

PBS

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: ME2

The ME2 gene encodes a mitochondrial NAD-dependent malic enzyme, a homotetrameric protein, that catalyzes the oxidative decarboxylation of malate to pyruvate. It had previously been weakly linked to a syndrome known as Friedreich ataxia that has since been s

Alternate Names

EC 1.1.1, EC 1.1.1.38, malate dehydrogenase, Malic enzyme 2, malic enzyme 2, NAD(+)-dependent, mitochondrial, NAD-dependent malic enzyme, mitochondrial, NAD-ME, ODS1, pyruvic-malic carboxylase

Gene Symbol

ME2

Additional ME2 Products

Product Documents for ME2 Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for ME2 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs

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