MED12 Antibody - BSA Free

Novus Biologicals | Catalog # NB100-2357

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Primate

Cited:

Human, Mouse, Primate

Predicted:

Chimpanzee (100%). Backed by our 100% Guarantee.

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunoprecipitation, Chromatin Immunoprecipitation (ChIP)

Cited:

Immunohistochemistry-Paraffin, Western Blot, Chemotaxis

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all MED12 Primary Antibodies »

Product Specifications

Immunogen

The immunogen recognized by this antibody maps to a region between residue 2150 and the C-terminus (residue 2212) of human Mediator of RNA Polymerase II Transcription, subunit 12 (NP_005111.1).

Reactivity Notes

Primate reactivity reported in scientific literature (PMID: 24147125).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

243 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for MED12 Antibody - BSA Free

Western Blot: MED12 Antibody [NB100-2357]

Western Blot: MED12 Antibody [NB100-2357]

MED12-Antibody-Western-Blot-NB100-2357-img0015.jpg
Immunohistochemistry: MED12 Antibody [NB100-2357]

Immunohistochemistry: MED12 Antibody [NB100-2357]

MED12-Antibody-Immunohistochemistry-NB100-2357-img0014.jpg
MED12 Antibody

Western Blot: MED12 Antibody [NB100-2357] -

Western Blot: MED12 Antibody [NB100-2357] - Samples: Whole cell lysate (10 ug) from NIH 3T3, CT26, CH27, TCMK-1, and BW5147.3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-Med12 antibody (NB100-2357 lot 3) used for WB at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.
MED12 Antibody

Western Blot: MED12 Antibody [NB100-2357] -

Western Blot: MED12 Antibody [NB100-2357] - Samples: Whole cell lysate (10 ug) from HeLa, A-549, GaMG, Hep-G2, and K-562 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-Med12 antibody (NB100-2357 lot 3) used for WB at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.
MED12 Antibody

Immunohistochemistry-Paraffin: MED12 Antibody [NB100-2357] -

Immunohistochemistry-Paraffin: MED12 Antibody [NB100-2357] - Sample: FFPE section of human prostate carcinoma. Antibody: Affinity purified rabbit anti-MED12 (NB100-2357 Lot 3) used at a dilution of 1:5,000 (0.2ug/ml). Detection: DAB
MED12 Antibody

Western Blot: MED12 Antibody [NB100-2357] -

Western Blot: MED12 Antibody [NB100-2357] - Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-Med12 antibody (NB100-2357 lot 3) used for IP at 6 ug per reaction. Med12 was also immunoprecipitated by a previous lot of this antibody (NB100-2357 lot 2) and a second antibody against a different epitope of Med12 (NBP2-80138). For blotting immunoprecipitated Med12, NB100-2357 was used at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 1 second.
MED12 Antibody

Immunohistochemistry-Paraffin: MED12 Antibody [NB100-2357] -

Immunohistochemistry-Paraffin: MED12 Antibody [NB100-2357] - Sample: FFPE section of mouse renal cell carcinoma. Antibody: Affinity purified rabbit anti-MED12 (NB100-2357 Lot 3) used at a dilution of 1:1,000 (1ug/ml). Detection: DAB

Applications for MED12 Antibody - BSA Free

Application
Recommended Usage

Immunohistochemistry

1:500- 1:2000

Immunohistochemistry-Paraffin

1:500 - 1:2000

Immunoprecipitation

2-10 ug/mg lysate

Western Blot

1:2000-1:10000
Application Notes
Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections. Use in chromatin immunoprecipitation reported in scientific literature (PMID: 31089260).

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris-Citrate/Phosphate (pH 7.0 - 8.0)

Format

BSA Free

Preservative

0.09% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: MED12

The initiation of transcription is controlled in part by a large protein assembly known as the preinitiation complex. A component of this preinitiation complex is a 1.2 MDa protein aggregate called Mediator. This Mediator component binds with a CDK8 subcomplex which contains the protein encoded by this gene, mediator complex subunit 12 (MED12), along with MED13, CDK8 kinase, and cyclin C. The CDK8 subcomplex modulates Mediator-polymerase II interactions and thereby regulates transcription initiation and reinitation rates. The MED12 protein is essential for activating CDK8 kinase. Defects in this gene cause X-linked Opitz-Kaveggia syndrome, also known as FG syndrome, and Lujan-Fryns syndrome. (provided by RefSeq)

Long Name

Mediator of RNA Polymerase II Transcription Subunit 12

Alternate Names

ARC240, CAGH45, FGS1, HOPA, OKS, TNRC11, TRAP230

Entrez Gene IDs

9968 (Human); 59024 (Mouse)

Gene Symbol

MED12

UniProt

Q93074

Additional MED12 Products

Product Documents for MED12 Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for MED12 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for MED12 Antibody - BSA Free

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Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs for MED12 Antibody - BSA Free

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  • Q: Could you tell me the differences between the two med12 antibodies for the IP? NB100-2357 in this image (image B, IP) you had written "Detection of Human and Mouse MED12 NB100-2357. MED12 was also immunoprecipitated by another rabbit anti-MED12 antibody Which is the other rabbit anti-med12 antibody? Maybe it's more specific for med12 (there is only one band, and no three bands like for NB100-2357).

    A: Unfortunately, the other MED12 antibody referred to in the image caption is not available for commercial sale. NB100-2357 displayed a higher affinity for MED12, which can be seen by the stronger signal strength, although it does detect degradation products of MED12, which are the two smaller bands on the blot.

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