MED17 Antibody (1B5) - Azide and BSA Free

Novus Biologicals | Catalog # H00009440-M02

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Western Blot, ELISA, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # 1B5

Format

Azide and BSA Free
View all MED17 Primary Antibodies »

Product Specifications

Immunogen

CRSP6 (AAH21101, 551 a.a. ~ 651 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. FSNHVGLGPIESIGNASAITVASPSGDYAISVRNGPESGSKIMVQFPRNQCKDLPKSDVLQDNKWSHLRGPFKEVQWNKMEGRNFVYKMELLMSALSPCLL

Specificity

CRSP6 (1B5)

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Scientific Data Images for MED17 Antibody (1B5) - Azide and BSA Free

Western Blot: MED17 Antibody (1B5) [H00009440-M02]

Western Blot: MED17 Antibody (1B5) [H00009440-M02]

Western Blot: MED17 Antibody (1B5) [H00009440-M02] - CRSP6 monoclonal antibody (M02), clone 1B5 Analysis of CRSP6 expression in Hela S3 NE.
Immunocytochemistry/ Immunofluorescence: MED17 Antibody (1B5) [H00009440-M02]

Immunocytochemistry/ Immunofluorescence: MED17 Antibody (1B5) [H00009440-M02]

Immunocytochemistry/Immunofluorescence: MED17 Antibody (1B5) [H00009440-M02] - Analysis of monoclonal antibody to CRSP6 on HeLa cell. Antibody concentration 10 ug/ml.
ELISA: MED17 Antibody (1B5) [H00009440-M02]

ELISA: MED17 Antibody (1B5) [H00009440-M02]

ELISA: MED17 Antibody (1B5) [H00009440-M02] - Detection limit for recombinant GST tagged CRSP6 is approximately 3ng/ml as a capture antibody.

Applications for MED17 Antibody (1B5) - Azide and BSA Free

Application
Recommended Usage

Western Blot

1:500
Application Notes
Antibody reactive against cell lysate and recombinant protein for Western Blot.

Reviewed Applications

Read 1 review rated 4 using H00009440-M02 in the following applications:

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: MED17

The activation of gene transcription is a multistep process that is triggered by factors that recognize transcriptional enhancer sites in DNA. These factors work with co-activators to direct transcriptional initiation by the RNA polymerase II apparatus. The protein encoded by this gene is a subunit of the CRSP (cofactor required for SP1 activation) complex, which, along with TFIID, is required for efficient activation by SP1. This protein is also a component of other multisubunit complexes e.g. thyroid hormone receptor-(TR-) associated proteins which interact with TR and facilitate TR function on DNA templates in conjunction with initiation factors and cofactors.

Alternate Names

Activator-recruited cofactor 77 kDa component, Cofactor required for Sp1 transcriptional activation subunit 6, cofactor required for Sp1 transcriptional activation, subunit 6, 77kDa, CRSP6FLJ10812, CRSP77cofactor required for Sp1 transcriptional activation, subunit 6 (77kD), DRIP77, DRIP80ARC77, mediator complex subunit 17Vitamin D3 receptor-interacting protein complex 80 kDa component, mediator of RNA polymerase II transcription subunit 17, Thyroid hormone receptor-associated protein complex 80 kDa component, Transcriptional coactivator CRSP77, Trap80, TRAP80CRSP complex subunit 6

Entrez Gene IDs

9440 (Human)

Gene Symbol

MED17

OMIM

603810 (Human)

UniProt

AAH21101 (Human)

Additional MED17 Products

Product Documents for MED17 Antibody (1B5) - Azide and BSA Free

Certificate of Analysis

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Product Specific Notices for MED17 Antibody (1B5) - Azide and BSA Free

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for MED17 Antibody (1B5) - Azide and BSA Free

Customer Reviews for MED17 Antibody (1B5) - Azide and BSA Free (1)

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  • Name: Anonymous
    Application: Western Blot
    Sample Tested: A549 nuclear lysate
    Species: Human
    Verified Customer | Posted 05/26/2016
    35 ug nuclear lysate per lane
    MED17 Antibody (1B5) - Azide and BSA Free H00009440-M02

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Protocols

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