MED4 Antibody - BSA Free

Novus Biologicals | Catalog # NBP1-84977

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human, Primate

Cited:

Primate

Predicted:

Mouse (90%), Rat (90%). Backed by our 100% Guarantee.

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all MED4 Primary Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: SGEKEKERLGGGLGVAGGNSTRERLLSALEDLEVLSRELIEMLAISRNQKLLQAGEENQVLELLIHRDGEFQELMKLALNQGKIHHEMQVLEKEVEKRDSDIQQLQKQLKEAEQI

Reactivity Notes

Primate reactivity reported in the scientific literature (PMID: 26067369).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for MED4 Antibody - BSA Free

Western Blot: MED4 Antibody [NBP1-84977]

Western Blot: MED4 Antibody [NBP1-84977]

MED4-Antibody-Western-Blot-NBP1-84977-img0008.jpg
Immunocytochemistry/ Immunofluorescence: MED4 Antibody [NBP1-84977]

Immunocytochemistry/ Immunofluorescence: MED4 Antibody [NBP1-84977]

Immunocytochemistry/Immunofluorescence: MED4 Antibody [NBP1-84977] - Staining of human cell line U-251 MG shows localization to nucleoplasm. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: MED4 Antibody [NBP1-84977]

Immunohistochemistry-Paraffin: MED4 Antibody [NBP1-84977]

Immunohistochemistry-Paraffin: MED4 Antibody [NBP1-84977] - Staining of human Placenta shows strong nuclear positivity in trophoblastic cells.
Western Blot: MED4 Antibody [NBP1-84977]

Western Blot: MED4 Antibody [NBP1-84977]

Western Blot: MED4 Antibody [NBP1-84977] - Analysis in control (vector only transfected HEK293T lysate) and MED4 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).
Immunohistochemistry-Paraffin: MED4 Antibody [NBP1-84977]

Immunohistochemistry-Paraffin: MED4 Antibody [NBP1-84977]

Immunohistochemistry-Paraffin: MED4 Antibody [NBP1-84977] - Staining of human stomach shows strong nuclear positivity in glandular cells.
Immunohistochemistry-Paraffin: MED4 Antibody [NBP1-84977]

Immunohistochemistry-Paraffin: MED4 Antibody [NBP1-84977]

Immunohistochemistry-Paraffin: MED4 Antibody [NBP1-84977] - Staining of human Cerebral cortex shows strong nuclear positivity in neuronal cells.
Immunohistochemistry-Paraffin: MED4 Antibody [NBP1-84977]

Immunohistochemistry-Paraffin: MED4 Antibody [NBP1-84977]

Immunohistochemistry-Paraffin: MED4 Antibody [NBP1-84977] - Staining of human Lymph node shows moderate nuclear positivity in non-germinal center and germinal center cells.
Immunohistochemistry-Paraffin: MED4 Antibody [NBP1-84977]

Immunohistochemistry-Paraffin: MED4 Antibody [NBP1-84977]

Immunohistochemistry-Paraffin: MED4 Antibody [NBP1-84977] - Staining of human Testis shows strong nuclear positivity in cells in seminiferous ducts.

Applications for MED4 Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:10-1:500

Immunohistochemistry-Paraffin

1:50-1:200

Western Blot

1:100-1:500
Application Notes
WB reported in scientific literature (PMID: 24147125). For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: MED4

MED4 is a component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymera

Long Name

Mediator Complex Subunit 4

Alternate Names

ARC36, DRIP36, HSPC126, TRAP36, VDRIP

Gene Symbol

MED4

Additional MED4 Products

Product Documents for MED4 Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for MED4 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for MED4 Antibody - BSA Free

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Protocols

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