MEKK1 Antibody - Azide and BSA Free

Novus Biologicals | Catalog # NBP3-02962

Novus Biologicals
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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

Azide and BSA Free
View all MEKK1 Primary Antibodies »

Product Specifications

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 1400-1512 of human MEKK1 (NP_005912.1). TGAGEFQGQLLGTIAFMAPEVLRGQQYGRSCDVWSVGCAIIEMACAKPPWNAEKHSNHLALIFKIASATTAPSIPSHLSPGLRDVALRCLELQPQDRPPSRELLKHPVFRTTW

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

164 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for MEKK1 Antibody - Azide and BSA Free

Western Blot: MEKK1 AntibodyAzide and BSA Free [NBP3-02962]

Western Blot: MEKK1 AntibodyAzide and BSA Free [NBP3-02962]

Western Blot: MEKK1 Antibody [NBP3-02962] - Analysis of extracts of various cell lines, using MEKK1 antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit
Immunocytochemistry/ Immunofluorescence: MEKK1 Antibody - Azide and BSA Free [NBP3-02962]

Immunocytochemistry/ Immunofluorescence: MEKK1 Antibody - Azide and BSA Free [NBP3-02962]

Immunocytochemistry/Immunofluorescence: MEKK1 Antibody [NBP3-02962] - Analysis of L929 cells using MEKK1 antibody at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
Immunohistochemistry-Paraffin: MEKK1 Antibody - Azide and BSA Free [NBP3-02962]

Immunohistochemistry-Paraffin: MEKK1 Antibody - Azide and BSA Free [NBP3-02962]

Immunohistochemistry-Paraffin: MEKK1 Antibody [NBP3-02962] - Mouse kidney using MEKK1 antibody (NBP3-02962) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
Immunohistochemistry-Paraffin: MEKK1 Antibody - Azide and BSA Free [NBP3-02962]

Immunohistochemistry-Paraffin: MEKK1 Antibody - Azide and BSA Free [NBP3-02962]

Immunohistochemistry-Paraffin: MEKK1 Antibody [NBP3-02962] - Human liver using MAP3K1 antibody at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
Immunohistochemistry-Paraffin: MEKK1 Antibody - Azide and BSA Free [NBP3-02962]

Immunohistochemistry-Paraffin: MEKK1 Antibody - Azide and BSA Free [NBP3-02962]

Immunohistochemistry-Paraffin: MEKK1 Antibody [NBP3-02962] - Rat liver using MAP3K1 antibody at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

Applications for MEKK1 Antibody - Azide and BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:50-1:100

Immunohistochemistry

1:50-1:100

Western Blot

1:500-1:2000

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS with 50% glycerol, pH7.3.

Format

Azide and BSA Free

Preservative

0.01% Thimerosal

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: MEKK1

MEKKs (Mitogen activated protein kinase kinase kinases) are serine-threonine kinases that act as the first tier of cellular MAP kinase pathways by activation of MAP/ERK kinases, or MEKs. Many enzymes with MEKK activity have been identified, including MEKK1-4, Raf, MLK3, TAK, and DLK. MEKKs generally display little similarity outside of their catalytic kinase domains. MEKK1-4 are nearly 50% identical within their catalytic domains, and are known to regulate Erk, Jnk, and p38 MAP kinase pathways. MEKK2 and MEKK3 bind MEK5 via conserved PB1 domains, leading to downstream activation of Erk5.

Alternate Names

Map3k1, MAPK/ERK kinase kinase 1, MAPKKK1MAP/ERK kinase kinase 1, MEK kinase 1, MEKK1EC 2.7.11.25, MEKKMEKK 1, mitogen-activated protein kinase kinase kinase 1

Gene Symbol

MAP3K1

Additional MEKK1 Products

Product Documents for MEKK1 Antibody - Azide and BSA Free

Certificate of Analysis

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Product Specific Notices for MEKK1 Antibody - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including Methotrexate, which is known to the State of California to cause reproductive toxicity with developmental effects. For more information, go to www.P65Warnings.ca.gov

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Protocols

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