Melanophilin Antibody

Name: Melanophilin Antibody
Brand: Novus Biologicals
SKU: NB300-943
Price: 399 USD
Availability: InStock

Novus Biologicals | Catalog # NB300-943

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Citations (2)

Product Documents

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Product Specifications
Scientific Data
Applications
Preparation & Storage
Background
Product Documents
Specific Notices

Key Product Details

Species Reactivity

Validated:

Mouse

Predicted:

Rat (100%). Backed by our 100% Guarantee.

Applications

Western Blot, Peptide ELISA, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG

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Product Specifications

Immunogen

Peptide with sequence C-ARHIFAKPVMAQQP corresponding to C-Terminus according to Uniprot Mouse NP_443748.2.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Melanophilin Antibody

Western Blot: Melanophilin Antibody [NB300-943]

Western Blot: Melanophilin Antibody [NB300-943] - Staining (0.5 ug/mL) of total melanocyte PNS (200 ug per lane) of wild-type and leaden (Mlph null) mice. The upper band may be the stacking-separating gel interface. Data kindly provided by A N Hume, Imperial College

Immunocytochemistry/ Immunofluorescence: Melanophilin Antibody [NB300-943]

Immunocytochemistry/Immunofluorescence: Melanophilin Antibody [NB300-943] - Wild-type C57bl6 derived melanocytes were stained with (0.5 ug/mL) and detected with Alexa568 conjugated donkey anti-sheep second antibodies. Brightfield image shows melanosome distribution. Data kindly provided by A N Hume, Imperial College.

Applications for Melanophilin Antibody

Application

Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.5 ug/mL

Peptide ELISA

Detection limit 1:32000

Western Blot

0.5 - 2 ug/mL

Application Notes

IF successful usage for stained mouse melanocytes is reported in scientific literature (PMID: 18559336). GST-pull down assay usage is reported in scientific literature(PMID: 18559336). WB: Approx. 80 kDa band observed in mouse Melanocyte extracts. Please note that this product may require overnight incubation of primary and high protein loads.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA

Preservative

0.02% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Melanophilin

Melanophilin is a Rab effector protein involved in melanosome transport. It serves as a link between melanosome-bound RAB27A and the motor protein MYO5A.

Alternate Names

exophilin-3, l(1)-3Rk, l1Rk3, ln, melanophilin, MGC2771, MGC59733, SLAC2A, Slac-2a, SLAC2-A, Slp homolog lacking C2 domains a, Synaptotagmin-like protein 2a

Entrez Gene IDs

79083 (Human); 171531 (Mouse); 316620 (Rat)

Gene Symbol

MLPH

UniProt

NP_443748.2 (Mouse)

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Product Documents for Melanophilin Antibody

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Certificate of Analysis

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Product Specific Notices for Melanophilin Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for Melanophilin Antibody

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