Metaxin 1 Antibody - BSA Free

Novus Biologicals | Catalog # NBP1-87741

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human

Predicted:

Mouse (93%), Rat (90%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
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Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: WIDTKNYVEVTRKWYAEAMPFPLNFFLPGRMQRQYMERLQLLTGEHRPEDEEELEKELYREARECLTLLSQRLGSQKFFFGDAPASLDAFVFSYLALLLQAKLPSG

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Metaxin 1 Antibody - BSA Free

Western Blot: Metaxin 1 Antibody [NBP1-87741]

Western Blot: Metaxin 1 Antibody [NBP1-87741]

Western Blot: Metaxin 1 Antibody [NBP1-87741] - Analysis in human cell line MCF-7.
Immunocytochemistry/ Immunofluorescence: Metaxin 1 Antibody [NBP1-87741]

Immunocytochemistry/ Immunofluorescence: Metaxin 1 Antibody [NBP1-87741]

Immunocytochemistry/Immunofluorescence: Metaxin 1 Antibody [NBP1-87741] - Staining of human cell line U-2 OS shows localization to mitochondria. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: Metaxin 1 Antibody [NBP1-87741]

Immunohistochemistry-Paraffin: Metaxin 1 Antibody [NBP1-87741]

Immunohistochemistry-Paraffin: Metaxin 1 Antibody [NBP1-87741] - Staining of human pancreas shows moderate granular cytoplasmic positivity in islets and Langerhans.
Immunohistochemistry-Paraffin: Metaxin 1 Antibody [NBP1-87741]

Immunohistochemistry-Paraffin: Metaxin 1 Antibody [NBP1-87741]

Immunohistochemistry-Paraffin: Metaxin 1 Antibody [NBP1-87741] - Staining of human testis shows strong granular cytoplasmic positivity in cells in seminiferous ducts and leydig cells.
Immunohistochemistry-Paraffin: Metaxin 1 Antibody [NBP1-87741]

Immunohistochemistry-Paraffin: Metaxin 1 Antibody [NBP1-87741]

Immunohistochemistry-Paraffin: Metaxin 1 Antibody [NBP1-87741] - Staining of human kidney shows strong granular cytoplasmic positivity in cells in tubules.
Immunohistochemistry-Paraffin: Metaxin 1 Antibody [NBP1-87741]

Immunohistochemistry-Paraffin: Metaxin 1 Antibody [NBP1-87741]

Immunohistochemistry-Paraffin: Metaxin 1 Antibody [NBP1-87741] - Staining of human rectum shows moderate granular cytoplasmic positivity in glandular cells.
Metaxin 1 Antibody - BSA Free

Western Blot: Metaxin 1 Antibody - BSA Free [NBP1-87741] -

Expression of key genes at mRNA and protein levels in the hippocampus of TLE animal model. (A) Construction of the TLE mouse model. It primarily illustrates the process of animal anesthesia and fixation, kainic acid injection localization, brain tissue separation on ice, and hippocampus extraction. (B) Expression levels of core genes' corresponding mRNA in the hippocampus of NC and TLE groups identified by qPCR. Data are shown as mean +/- SE. (C) Western blot analysis of hippocampal lysates displaying the protein levels of Dnmt1, Cdc25b, Ssh2, Fgd3, Gzma, Raf1, and Mx1 with Actin serving as a loading control. Approximate molecular weights are indicated. (D) Quantification of Western blot analysis of the protein bands in Figure C, through relative gray values compared across NC and TLE hippocampus samples. (E) Quantitative analyses of immunofluorescence staining showing the fluorescence intensity between NC and TLE in Figure F. (F) Representative immunofluorescence images showing the hippocampal localization of Cdc25b, Raf1, Fgd3, Dnmt1, Mx1, and Ssh2 (red) with DAPI staining the nuclei (blue) in NC and TLE groups (Scale bar, 100 μm). (TLE, temporal lobe epilepsy; NC, normal control. *p < 0.05, **p < 0.01, ***p < 0.001, ns, not significant.) Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/39753851), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Applications for Metaxin 1 Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:20 - 1:50

Immunohistochemistry-Paraffin

1:20 - 1:50

Western Blot

0.04 - 0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF, Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Metaxin 1

Metaxin 1 is involved in transport of proteins into the mitochondrion. Essential for embryonic development

Alternate Names

metaxin 1, metaxin-1, MTX, MTXNMitochondrial outer membrane import complex protein 1

Gene Symbol

MTX1

Additional Metaxin 1 Products

Product Documents for Metaxin 1 Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for Metaxin 1 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for Metaxin 1 Antibody - BSA Free

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Protocols

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FAQs

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