Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat, Chicken

Cited:

Mouse, Rat

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen, Western Blot, Immunocytochemistry/ Immunofluorescence

Cited:

Immunohistochemistry-Paraffin, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all mGluR1 Primary Antibodies »

Product Specifications

Immunogen

Synthetic peptide comprising residues 1159-1171 [SSVPSSPVSESVL] of the human mGluR1 protein.

Localization

Cell membrane

Specificity

Glutamate Receptor

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

132.4 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

Novus Biologicals Rabbit mGluR1 Antibody - BSA Free (NB110-39033) is a polyclonal antibody validated for use in IHC, WB and ICC/IF. Anti-mGluR1 Antibody: Cited in 3 publications. All Novus Biologicals antibodies are covered by our 100% guarantee.

Scientific Data Images for mGluR1 Antibody - BSA Free

Western Blot: mGluR1 AntibodyBSA Free [NB110-39033]

Western Blot: mGluR1 AntibodyBSA Free [NB110-39033]

Western Blot: mGluR1 Antibody [NB110-39033] - WB detection of GluR1 in 20 ug of human brain lysate (lane 2) with GluR1 polyclonal antibody at 1:1000 dilution followed by AP-conjugated secondary at 1:5000 dilution. Lane 1 represents the MW marker.
Immunocytochemistry/ Immunofluorescence: mGluR1 Antibody - BSA Free [NB110-39033]

Immunocytochemistry/ Immunofluorescence: mGluR1 Antibody - BSA Free [NB110-39033]

Immunocytochemistry/Immunofluorescence: mGluR1 Antibody [NB110-39033] - Primary Rat Cortical Neurons, image courtesy of customer.
Immunohistochemistry-Frozen: mGluR1 Antibody - BSA Free [NB110-39033]

Immunohistochemistry-Frozen: mGluR1 Antibody - BSA Free [NB110-39033]

Immunohistochemistry-Frozen: mGluR1 Antibody [NB110-39033] - Immunofluorescence labeling for GluR1 in rat brain (cingulate cortex). GluR1 detection was done using anti-rabbit Cy3 conjugated antibodies (red color). DAPI was used to counterstain cell nuclei (blue color). Working dilution is 1:100-1:500.

Applications for mGluR1 Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100-1:500

Immunohistochemistry

1:100-1:500

Immunohistochemistry-Frozen

1:200

Immunohistochemistry-Paraffin

1:100-1:500

Western Blot

1:500-1:1000
Application Notes
This metabotropic Glutamate Receptor 1a antibody is useful for Western Blot, Immunohistochemistry-Frozen and Immunohistochemistry-paraffin applications. Use in Immunocytochemistry/immunofluorescence was reported in scientific literature (PMID: 19688350) The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reviewed Applications

Read 1 review rated 4 using NB110-39033 in the following applications:

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: mGluR1

The Metabotropic Glutamate Receptor mGluR1 binds L-glutamate, the major excitatory neurotransmitter in the central nervous system, which activates both ionotropic and metabotropic glutamate receptors. Glutamatergic neurotransmission is involved in most aspects of normal brain function and can be perturbed in many neuropathologic conditions. mGluR1 and mGluR5 are group I receptors that have been shown to activate phospholipase C. Alternative splice variants of the mGluR1 gene have been described, but their full-length nature has not been determined. Inflammation results in the activation of mGluR1 and mGluR5 in dorsal horn neurons of the spinal cord, leading to extracellular signal-regulated kinase (ERK) activation, which is required for nociceptive plasticity and enhanced pain. mGluR1 has been reported in various regions of the brain and spinal cord. ESTs have been isolated from brain and eye libraries. Caution: GRIA1 refers to Ionotropic Glutamate Receptor 1, not to Metabotropic Glutamate Receptor 1 (mGLUR1).

Long Name

Metabotropic Glutamate Receptor 1

Alternate Names

GPRC1A, GRM1

Entrez Gene IDs

2911 (Human); 14810 (Mouse); 24408 (Rat)

Gene Symbol

GRM1

UniProt

Q13255

Additional mGluR1 Products

Product Documents for mGluR1 Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for mGluR1 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for mGluR1 Antibody - BSA Free

Customer Reviews for mGluR1 Antibody - BSA Free (1)

4 out of 5
1 Customer Rating
5 Stars
0%
4 Stars
100%
3 Stars
0%
2 Stars
0%
1 Stars
0%

Have you used mGluR1 Antibody - BSA Free?

Submit a review and receive an Amazon gift card!

$25/€18/£15/$25CAN/¥2500 Yen for a review with an image

$10/€7/£6/$10CAN/¥1110 Yen for a review without an image

Submit a review
Amazon Gift Card

Customer Images

Showing  1 - 1 of 1 review Showing All
Filter By:
Clear Filters X
  • metabotropic Glutamate Receptor 1a Antibody
    Name: Janosch Heller
    Application: Immunocytochemistry
    Sample Tested: Primary rat cortical neurons
    Species: Rat
    Verified Customer | Posted 03/27/2017
    Fixation: 4% paraformaldehyde (TAAB) in PBS, 15 minutes RT Quenching: 0.1% NaBH4 in PBS, 7 minutes RT Blocking: 3% BSA in PBS, 30 minutes RT Permeabilization: 0.1% Triton-X100, 10 minutes RT Primary Antibody: 1:500 in 3% BSA in PBS, overnight, 4°C Wash: three times wash with PBS, 10 min each Secondary Antibody: Invitrogen goat anti-mouse Alexa568, 1:1800 in PBS with 1% BSA, 2 hours, RT Wash: three times wash with PBS, 10 min each Microscope: Bruker Vutara 350 The antibody labels clusters of metabotropic receptors along the whole cell.
    mGluR1 Antibody - BSA Free NB110-39033

There are no reviews that match your criteria.

Showing  1 - 1 of 1 review Showing All

Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs

No product specific FAQs exist for this product.

View all FAQs for Antibodies