MGME1 Antibody - Azide and BSA Free
Novus Biologicals | Catalog # NBP3-23534
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Key Product Details
Species Reactivity
Human
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Format
Azide and BSA Free
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Product Specifications
Immunogen
Produced in rabbits immunized with E. coli-derived Human MGME1 fragment
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Description
This antibody can be stored at 2C-8C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20C to -80C.
Scientific Data Images for MGME1 Antibody - Azide and BSA Free
Immunoprecipitation: MGME1 Antibody - Azide and BSA Free [NBP3-23534] -
Immunoprecipitation: MGME1 Antibody - Azide and BSA Free [NBP3-23534] - C20orf72 was immunoprecipitated using: Lane A:0.5 mg HepG2 Whole Cell Lysate 4 µL NBP3-23534 and 60 ug of Immunomagnetic beads Protein A/G. Primary antibody: NBP3-23534,at 1:100 dilution Secondary antibody: Goat Anti-Rabbit IgG (H+L) [HRP] at 1/10000 dilution Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 39 kDa Observed band size :35 kDa
Immunohistochemistry-Paraffin: MGME1 Antibody - Azide and BSA Free [NBP3-23534] -
Immunohistochemistry-Paraffin: MGME1 Antibody - Azide and BSA Free [NBP3-23534] - Immunochemical staining of human MGME1 in human kidney with NBP3-23534 at 1:100 dilution, formalin-fixed paraffin embedded sections.
Western Blot: MGME1 Antibody - Azide and BSA Free [NBP3-23534] -
Western Blot: MGME1 Antibody - Azide and BSA Free [NBP3-23534] - NBP3-23534 at 1:500 dilution. Lane A: HepG2 Whole Cell Lysate. Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L) [HRP] at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 39 kDa. Observed band size: 35 kDa
Immunohistochemistry-Paraffin: MGME1 Antibody [NBP3-23534] -
Immunohistochemistry-Paraffin: MGME1 Antibody [NBP3-23534] - Immunochemical staining of human MGME1 in human rectum with NBP3-23534 at 1:100 dilution, formalin-fixed paraffin embedded sections.
Immunocytochemistry/ Immunofluorescence: MGME1 Antibody [NBP3-23534] -
Immunocytochemistry/ Immunofluorescence: MGME1 Antibody [NBP3-23534] - Immunofluorescence staining of MGME1 in U2OS cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS, blocked with 10% serum, and incubated with NBP3-23534 (dilution ratio 1:100) at 4C overnight. Then cells were stained with the Goat Anti-rabbit IgG secondary antibody [Alexa Fluor 488] (green) and counterstained with DAPI (blue). Positive staining was localized to Cytoplasm.Applications for MGME1 Antibody - Azide and BSA Free
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
1:50-1:200
Immunohistochemistry-Paraffin
1:50-1:200
Immunoprecipitation
1-5 uL/mg of lysate
Western Blot
1:500-1:2000
Formulation, Preparation, and Storage
Purification
Antigen and protein A Affinity-purified
Formulation
0.2 um filtered solution in PBS
Format
Azide and BSA Free
Preservative
No Preservative
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: MGME1
Alternate Names
bA504H3.4, chromosome 20 open reading frame 72, FLJ14597, hypothetical protein LOC92667
Gene Symbol
MGME1
Additional MGME1 Products
Product Documents for MGME1 Antibody - Azide and BSA Free
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for MGME1 Antibody - Azide and BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Antigen Retrieval Protocol (PIER)
- Antigen Retrieval for Frozen Sections Protocol
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- Chromogenic IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Chromogenic Immunohistochemistry Staining of Frozen Tissue
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- Fluorescent IHC Staining of Frozen Tissue Protocol
- Graphic Protocol for Heat-induced Epitope Retrieval
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Graphic Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- ICC Cell Smear Protocol for Suspension Cells
- ICC Immunocytochemistry Protocol Videos
- ICC for Adherent Cells
- IHC Sample Preparation (Frozen sections vs Paraffin)
- Immunocytochemistry (ICC) Protocol
- Immunocytochemistry Troubleshooting
- Immunofluorescence of Organoids Embedded in Cultrex Basement Membrane Extract
- Immunofluorescent IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunohistochemistry Frozen Troubleshooting
- Immunohistochemistry Paraffin Troubleshooting
- Immunoprecipitation Protocol
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Protocol for Heat-Induced Epitope Retrieval (HIER)
- Protocol for Making a 4% Formaldehyde Solution in PBS
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Fluorescent ICC Staining of Cell Smears - Graphic
- Protocol for the Fluorescent ICC Staining of Cultured Cells on Coverslips - Graphic
- Protocol for the Preparation & Fixation of Cells on Coverslips
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections - Graphic
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections - Graphic
- Protocol for the Preparation and Fluorescent ICC Staining of Cells on Coverslips
- Protocol for the Preparation and Fluorescent ICC Staining of Non-adherent Cells
- Protocol for the Preparation and Fluorescent ICC Staining of Stem Cells on Coverslips
- Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- Protocol for the Preparation of a Cell Smear for Non-adherent Cell ICC - Graphic
- R&D Systems Quality Control Western Blot Protocol
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: Immunohistochemistry
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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