MHC Class II RT1B Antibody (OX-6) - Azide and BSA Free

Novus Biologicals | Catalog # NBP2-80849

Novus Biologicals

Key Product Details

Species Reactivity

Mouse, Rat

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen, Western Blot, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, CyTOF-ready

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # OX-6

Format

Azide and BSA Free
View all MHC Class II RT1B Primary Antibodies »

Product Specifications

Immunogen

Rat thymocyte membrane glycoproteins

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for MHC Class II RT1B Antibody (OX-6) - Azide and BSA Free

Western Blot: MHC Class II RT1B Antibody (OX-6)Azide and BSA Free [NBP2-80849]

Western Blot: MHC Class II RT1B Antibody (OX-6)Azide and BSA Free [NBP2-80849]

Western Blot: MHC Class II RT1B Antibody (OX-6) - Azide and BSA Free [NBP2-80849] - Total protein from mouse placenta and spleen was separated on a 12% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 2.0 ug/ml anti-RT1b in 1% milk, and detected with an anti-mouse HRP secondary antibody using chemiluminescence. Image from the standard format of this antibody.
Immunocytochemistry: MHC Class II RT1B Antibody (OX-6) - Azide and BSA Free [NBP2-80849]

Immunocytochemistry: MHC Class II RT1B Antibody (OX-6) - Azide and BSA Free [NBP2-80849]

Immunocytochemistry: MHC Class II RT1B Antibody (OX-6) - Azide and BSA Free [NBP2-80849] - IHC analysis of a formalin fixed and paraffin embedded tissue section of mouse spleen using MHC Class II RT1B antibody (clone OX-6) at 1:100 dilution. The signal was developed using HRP-labelled secondary antibody and DAB reagent, and the nuclei were coun
Flow Cytometry: MHC Class II RT1B Antibody (OX-6) - Azide and BSA Free [NBP2-80849]

Flow Cytometry: MHC Class II RT1B Antibody (OX-6) - Azide and BSA Free [NBP2-80849]

Flow Cytometry: MHC Class II RT1B Antibody (OX-6) - Azide and BSA Free [NBP2-80849] - Staining of rat splenocytes with Mouse anti Rat MHC class II RT1B Image from the standard format of this antibody.
Western Blot: MHC Class II RT1B Antibody (OX-6)Azide and BSA Free [NBP2-80849]

Western Blot: MHC Class II RT1B Antibody (OX-6)Azide and BSA Free [NBP2-80849]

Western Blot: MHC Class II RT1B Antibody (OX-6) - Azide and BSA Free [NBP2-80849] - Rat Spleen Tissue lysate probed with MHC Class II antibody. Image from the standard format of this antibody.
Flow Cytometry: MHC Class II RT1B Antibody (OX-6) - Azide and BSA Free [NBP2-80849]

Flow Cytometry: MHC Class II RT1B Antibody (OX-6) - Azide and BSA Free [NBP2-80849]

Flow Cytometry: MHC Class II RT1B Antibody (OX-6) - Azide and BSA Free [NBP2-80849] - Analysis using Alexa Fluor (R) 488 conjugate of NB100-65541. Rat Splenocytes were stained with MHC Class II RT1B (OX-6) antibody NB100-65541 (blue) and a matched isotype control NBP2-27287 (orange). Cells were incubated in an antibody dilution of 1 ug/mL for 20 minutes at room temperature. The antibodies were directly conjugated to Alexa Fluor 488.
Flow Cytometry: MHC Class II RT1B Antibody (OX-6) - Azide and BSA Free [NBP2-80849]

Flow Cytometry: MHC Class II RT1B Antibody (OX-6) - Azide and BSA Free [NBP2-80849]

Flow Cytometry: MHC Class II RT1B Antibody (OX-6) - Azide and BSA Free [NBP2-80849] - Staining of rat spleen cells. Image from the standard format of this antibody.
Flow Cytometry: MHC Class II RT1B Antibody (OX-6) - Azide and BSA Free [NBP2-80849]

Flow Cytometry: MHC Class II RT1B Antibody (OX-6) - Azide and BSA Free [NBP2-80849]

Flow Cytometry: MHC Class II RT1B Antibody (OX-6) - Azide and BSA Free [NBP2-80849] - Staining of rat spleen cells. Image from the standard format of this antibody.
Flow Cytometry: MHC Class II RT1B Antibody (OX-6) - Azide and BSA Free [NBP2-80849]

Flow Cytometry: MHC Class II RT1B Antibody (OX-6) - Azide and BSA Free [NBP2-80849]

Flow Cytometry: MHC Class II RT1B Antibody (OX-6) - Azide and BSA Free [NBP2-80849] - Staining of rat spleen cells. Image from the standard format of this antibody.
Flow Cytometry: MHC Class II RT1B Antibody (OX-6) - Azide and BSA Free [NBP2-80849]

Flow Cytometry: MHC Class II RT1B Antibody (OX-6) - Azide and BSA Free [NBP2-80849]

Flow Cytometry: MHC Class II RT1B Antibody (OX-6) - Azide and BSA Free [NBP2-80849] - Staining of rat spleen cells. Image from the standard format of this antibody.

Applications for MHC Class II RT1B Antibody (OX-6) - Azide and BSA Free

Application
Recommended Usage

Flow Cytometry

1:50-1:100

Immunocytochemistry/ Immunofluorescence

1:10-1:500

Immunohistochemistry

1:10-1:500

Immunohistochemistry-Frozen

1:10-1:500

Immunohistochemistry-Paraffin

1:50-1:100

Western Blot

1:100-1:2000
Application Notes
For IHC-Paraffin: This product requires antigen retrieval using heat treatment prior to staining of paraffin sections. Sodium citrate buffer pH 6.0 is recommended for this purpose. PLP fixation is recommended for optimal results. This antibody is CyTOF ready.

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: MHC Class II RT1B

MHC Class II antigens are heterodimers consisting of one alpha chain(31-34kD) and one beta chain (26-29kD). The family of monoclonal antibodies (ER-TR 3, ER-TR 2, ER-TR 1) detect MHC class II antigens encoded by the murine Ia region of the H-2 complex, corresponding to the human HLA-DR region. MHC Class II antigens are a valuable tool for studying T helper cell interaction with class II positive antigen presenting cells (dendritic cells, B cells, macrophages) and offer new possibilities for studying the development of T helper cells since these antibodies also stain stromal cells in the thymus. MHC Class II antigens are also inducible on a number of other cells (endothelium and epithelial cells) by interferon gamma.

Alternate Names

DMA, DMB, DP beta 1, DPB 1, DPB1, DRB, HLA class II histocompatibility antigen DM beta chain, HLA DMB, HLA DPB1, HLADM, HLADP1B, Major histocompatibility complex class II, Major histocompatibility complex class II DP beta 1, MHC class II antigen DMB, MHC DPB1, RING6, RING7

Gene Symbol

RT1-B

Additional MHC Class II RT1B Products

Product Documents for MHC Class II RT1B Antibody (OX-6) - Azide and BSA Free

Certificate of Analysis

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Product Specific Notices for MHC Class II RT1B Antibody (OX-6) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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