MHC Class II RT1B Antibody (OX-6) - BSA Free

Novus Biologicals | Catalog # NB100-65541

Novus Biologicals

Key Product Details

Validated by

Biological Validation

Species Reactivity

Validated:

Mouse, Rat

Cited:

Mouse, Rat

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen, Immunohistochemistry Free-Floating, Western Blot, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, CyTOF-ready

Cited:

Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen, Immunohistochemistry Free-Floating, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, IF/IHC

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # OX-6

Format

BSA Free
View all MHC Class II RT1B Primary Antibodies »

Product Specifications

Immunogen

Rat thymocyte membrane glycoproteins

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.

Specificity

NB100-65541 recognizes a monomorphic determinant of the rat I-A antigen present on B lymphocytes, dendritic cells, some macrophages and certain epithelial cells. This antibody cross reacts with certain mouse strains of MHC haplotypes k and s. This antibody does not react with C57BL6 or C57BL6/j mice because they are haplotype b. Analysis of recombinant mouse strains showed that the determinants mapped to the I-A region. The clone does not react with Rat BDIX strain. Please be aware of the possible size variance seen in western blot.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Description

Novus Biologicals Mouse MHC Class II RT1B Antibody (OX-6) - BSA Free (NB100-65541) is a monoclonal antibody validated for use in IHC, WB, Flow and ICC/IF. Anti-MHC Class II RT1B Antibody: Cited in 19 publications. All Novus Biologicals antibodies are covered by our 100% guarantee.

Scientific Data Images for MHC Class II RT1B Antibody (OX-6) - BSA Free

Immunohistochemistry: MHC Class II RT1B Antibody (OX-6) - BSA Free [NB100-65541]

Immunohistochemistry: MHC Class II RT1B Antibody (OX-6) - BSA Free [NB100-65541]

MHC-Class-II-RT1B-Antibody-OX-6-Immunohistochemistry-NB100-65541-img0017.jpg
Western Blot: MHC Class II RT1B Antibody (OX-6)BSA Free [NB100-65541]

Western Blot: MHC Class II RT1B Antibody (OX-6)BSA Free [NB100-65541]

Western Blot: MHC Class II RT1B Antibody (OX-6) [NB100-65541] - Total protein from mouse placenta and spleen was separated on a 12% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 2.0 ug/ml anti-RT1b in 1% milk, and detected with an anti-mouse HRP secondary antibody using chemiluminescence.
Immunohistochemistry-Paraffin: MHC Class II RT1B Antibody (OX-6) - BSA Free [NB100-65541]

Immunohistochemistry-Paraffin: MHC Class II RT1B Antibody (OX-6) - BSA Free [NB100-65541]

Immunohistochemistry-Paraffin: MHC Class II RT1B Antibody (OX-6) [NB100-65541] - IHC analysis of a formalin fixed and paraffin embedded tissue section of mouse spleen using MHC Class II RT1B antibody (clone OX-6) at 1:100 dilution. The signal was developed using HRP-labelled secondary antibody and DAB reagent, and the nuclei were counterstained with hematoxylin. The antibody generated very specific signal in a subset of spleenocytes.
Flow Cytometry: MHC Class II RT1B Antibody (OX-6) - BSA Free [NB100-65541]

Flow Cytometry: MHC Class II RT1B Antibody (OX-6) - BSA Free [NB100-65541]

Flow Cytometry: MHC Class II RT1B Antibody (OX-6) [NB100-65541] - Staining of rat spleen cells.
Immunohistochemistry: MHC Class II RT1B Antibody (OX-6) - BSA Free [NB100-65541]

Immunohistochemistry: MHC Class II RT1B Antibody (OX-6) - BSA Free [NB100-65541]

MHC-Class-II-RT1B-Antibody-OX-6-Immunohistochemistry-NB100-65541-img0019.jpg
Flow Cytometry: MHC Class II RT1B Antibody (OX-6) - BSA Free [NB100-65541]

Flow Cytometry: MHC Class II RT1B Antibody (OX-6) - BSA Free [NB100-65541]

Flow Cytometry: MHC Class II RT1B Antibody (OX-6) [NB100-65541] - Analysis using Alexa Fluor (R) 488 conjugate of NB100-65541. Rat Splenocytes were stained with MHC Class II RT1B (OX-6) antibody NB100-65541 (blue) and a matched isotype control NBP2-27287 (orange). Cells were incubated in an antibody dilution of 1 ug/mL for 20 minutes at room temperature. The antibodies were directly conjugated to Alexa Fluor 488.
Western Blot: MHC Class II RT1B Antibody (OX-6)BSA Free [NB100-65541]

Western Blot: MHC Class II RT1B Antibody (OX-6)BSA Free [NB100-65541]

Western Blot: MHC Class II RT1B Antibody (OX-6) [NB100-65541] - Rat Spleen Tissue lysate probed with MHC Class II antibody.
MHC Class II RT1B Antibody (OX-6) - BSA Free

Flow Cytometry: MHC Class II RT1B Antibody (OX-6) - BSA Free [NB100-65541] -

Flow Cytometry: MHC Class II RT1B Antibody (OX-6) - BSA Free [NB100-65541] - Staining of rat splenocytes with Mouse anti Rat MHC class II RT1B
MHC Class II RT1B Antibody (OX-6) - BSA Free

Flow Cytometry: MHC Class II RT1B Antibody (OX-6) - BSA Free [NB100-65541] -

Flow Cytometry: MHC Class II RT1B Antibody (OX-6) - BSA Free [NB100-65541] - Staining of rat spleen cells.
MHC Class II RT1B Antibody (OX-6) - BSA Free

Immunocytochemistry/ Immunofluorescence: MHC Class II RT1B Antibody (OX-6) - BSA Free [NB100-65541] -

Immunocytochemistry/ Immunofluorescence: MHC Class II RT1B Antibody (OX-6) - BSA Free [NB100-65541] - Administration of cord blood cells with plasma in the 6-OHDA PD rat model: Assessment of immune cell activation, & pro-inflammatory cytokine production in the intestine. Confocal imaging shows a significant reduction in OX-6 (MHC II) expression in the intestinal mucosa of 6-OHDA animals that were given a combination of cord blood cells & plasma. This therapy also resulted in a significant decrease in TNF expression in this animal model. Quantitative analyses of the estimated OX-6 expression & TNF are displayed in the graphs above. Representative merged images above show co-localization of OX-6 (Green) or TNF (Red) with DAPI+ (Blue) expression from cells in the small intestine of 6-OHDA PD animals. Arrow heads indicate positive staining of OX-6 & TNF expression in intestinal villi. Scale bar = 100 μm Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/31317505), licensed under a CC-BY license. Not internally tested by Novus Biologicals.
MHC Class II RT1B Antibody (OX-6) - BSA Free

Immunocytochemistry/ Immunofluorescence: MHC Class II RT1B Antibody (OX-6) - BSA Free [NB100-65541] -

Immunocytochemistry/ Immunofluorescence: MHC Class II RT1B Antibody (OX-6) - BSA Free [NB100-65541] - Administration of cord blood cells with plasma in the 6-OHDA PD rat model: Assessment of TH-positive dopaminergic neurons, immune cell activation, & pro-inflammatory cytokine production. Tyrosine hydroxylase (TH) staining (top row) was used to investigate the potential effect of combined cord blood cell with plasma injection on dopaminergic neuron populations in the substantia nigra pars compacta (SNpc). Analysis of fluorescent intensity revealed significant protection & retention of dopaminergic cells in the SNpc in 6-OHDA animals treated with cord blood cells & plasma, compared to vehicle controls. Cord blood treated groups also demonstrated significant reductions immune cell activation (OX-6) & pro-inflammatory cytokine production (TNF). Overall, the combination of cord blood cells & plasma can significantly modulate the exacerbated immune response through downregulation of pro-inflammatory cytokine production & immune cell activation in the 6-OHDA rat model at all time points tested. Photomicrographs correspond to representative SN in coronal sections immune-labeled with TH, OX-6 or TNF antibody. Scale bar = 50 μm Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/31317505), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Applications for MHC Class II RT1B Antibody (OX-6) - BSA Free

Application
Recommended Usage

Flow Cytometry

1:50-1:100

Immunocytochemistry/ Immunofluorescence

1:10-1:500

Immunohistochemistry

1:10-1:500

Immunohistochemistry Free-Floating

reported in scientific literature (PMID 31317505)

Immunohistochemistry-Frozen

1:10-1:500

Immunohistochemistry-Paraffin

1:50-1:100

Western Blot

1:100-1:2000
Application Notes
For IHC-Paraffin: This product requires antigen retrieval using heat treatment prior to staining of paraffin sections. Sodium citrate buffer pH 6.0 is recommended for this purpose. PLP fixation is recommended for optimal results. This antibody is CyTOF ready.

Reviewed Applications

Read 1 review rated 3 using NB100-65541 in the following applications:

Flow Cytometry Panel Builder

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: MHC Class II RT1B

MHC Class II antigens are heterodimers consisting of one alpha chain(31-34kD) and one beta chain (26-29kD). The family of monoclonal antibodies (ER-TR 3, ER-TR 2, ER-TR 1) detect MHC class II antigens encoded by the murine Ia region of the H-2 complex, corresponding to the human HLA-DR region. MHC Class II antigens are a valuable tool for studying T helper cell interaction with class II positive antigen presenting cells (dendritic cells, B cells, macrophages) and offer new possibilities for studying the development of T helper cells since these antibodies also stain stromal cells in the thymus. MHC Class II antigens are also inducible on a number of other cells (endothelium and epithelial cells) by interferon gamma.

Alternate Names

DMA, DMB, DP beta 1, DPB 1, DPB1, DRB, HLA class II histocompatibility antigen DM beta chain, HLA DMB, HLA DPB1, HLADM, HLADP1B, Major histocompatibility complex class II, Major histocompatibility complex class II DP beta 1, MHC class II antigen DMB, MHC DPB1, RING6, RING7, anti-OX-6 RT1B, anti-RT1B OX-6, Clone OX6, MHC class II DQ, Non-polymorphic MHC Class II, RT1B

Entrez Gene IDs

14998 (Mouse); 294274 (Rat)

Gene Symbol

RT1-B

Additional MHC Class II RT1B Products

Product Documents for MHC Class II RT1B Antibody (OX-6) - BSA Free

Certificate of Analysis

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Product Specific Notices for MHC Class II RT1B Antibody (OX-6) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for MHC Class II RT1B Antibody (OX-6) - BSA Free

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    • A: I heard back from our lab to your question on haplotype vs reactivity of MHC Class II Antibody (OX-6) [NB100-65541]. He told me that we would not expect this antibody to recognize C57BL6 or C57BL6/j since they are haplotype b.
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