MIF Antibody (4E4) - BSA Free
Novus Biologicals | Catalog # NBP1-04319
![Western Blot: MIF Antibody (4E4)BSA Free [NBP1-04319]](https://beta-resources.rndsystems.com/images/products/MIF-Antibody-4E4-Western-Blot-NBP1-04319-img0002.jpg "Western Blot: MIF Antibody (4E4)BSA Free [NBP1-04319]")
Key Product Details
Species Reactivity
Validated:
Human
Cited:
Human
Applications
Validated:
Immunohistochemistry, Western Blot, ELISA, Immunocytochemistry/ Immunofluorescence
Cited:
IF/IHC
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 kappa Clone # 4E4
Format
BSA Free
Loading...
Product Specifications
Immunogen
Recombinant human MIF (1-115 aa) purified from E. coli
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1 kappa
Description
Novus Biologicals Mouse MIF Antibody (4E4) - BSA Free (NBP1-04319) is a monoclonal antibody validated for use in IHC, WB, ELISA and ICC/IF. Anti-MIF Antibody: Cited in 1 publication. All Novus Biologicals antibodies are covered by our 100% guarantee.
Scientific Data Images for MIF Antibody (4E4) - BSA Free
Western Blot: MIF Antibody (4E4)BSA Free [NBP1-04319]
Western Blot: MIF Antibody (4E4) [NBP1-04319] - The cell lysates of HL-60 was resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human MIF antibody (1:1,000). Protein was visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.![Immunocytochemistry/ Immunofluorescence: MIF Antibody (4E4) - BSA Free [NBP1-04319]](https://beta-resources.rndsystems.com/images/products/MIF-Antibody-4E4-Immunocytochemistry-Immunofluorescence-NBP1-04319-img0004.jpg "Immunocytochemistry/ Immunofluorescence: MIF Antibody (4E4) - BSA Free [NBP1-04319]")
Immunocytochemistry/ Immunofluorescence: MIF Antibody (4E4) - BSA Free [NBP1-04319]
Immunocytochemistry/Immunofluorescence: MIF Antibody (4E4) [NBP1-04319] - Analysis of MIF in HeLa cells. The cell was stained at (1:100). The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue).![Western Blot: MIF Antibody (4E4)BSA Free [NBP1-04319]](https://beta-resources.rndsystems.com/images/products/MIF-Antibody-4E4-Western-Blot-NBP1-04319-img0001.jpg "Western Blot: MIF Antibody (4E4)BSA Free [NBP1-04319]")
Western Blot: MIF Antibody (4E4)BSA Free [NBP1-04319]
Western Blot: MIF Antibody (4E4) [NBP1-04319] - The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human MIF antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.Lane 1.: Jurkat cell lysateLane 2.: THP-1 cell lysateLane 3.: HeLa cell lysateLane 4.: U937 cell lysate![Immunocytochemistry/ Immunofluorescence: MIF Antibody (4E4) - BSA Free [NBP1-04319]](https://beta-resources.rndsystems.com/images/products/MIF-Antibody-4E4-Immunocytochemistry-Immunofluorescence-NBP1-04319-img0003.jpg "Immunocytochemistry/ Immunofluorescence: MIF Antibody (4E4) - BSA Free [NBP1-04319]")
Immunocytochemistry/ Immunofluorescence: MIF Antibody (4E4) - BSA Free [NBP1-04319]
Immunocytochemistry/Immunofluorescence: MIF Antibody (4E4) [NBP1-04319] - Analysis of MIF in Balb/3T3 cells. The cell was stained at (1:100). The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue).Applications for MIF Antibody (4E4) - BSA Free
Application
Recommended Usage
ELISA
1:100-1:2000
Western Blot
1:500-1:2000
Application Notes
Use in IHC reported in scientific literature (PMID:34514093).
Formulation, Preparation, and Storage
Purification
Protein G purified
Formulation
PBS (pH 7.4), 10% Glycerol
Format
BSA Free
Preservative
0.02% Sodium Azide
Concentration
1.0 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: MIF
Long Name
Macrophage Migration Inhibitory Factor
Alternate Names
EC 5.3.2.1, EC 5.3.3.12, GIFmacrophage migration inhibitory factor, GLIF, Glycosylation-inhibiting factor, L-dopachrome isomerase, L-dopachrome tautomerase, macrophage migration inhibitory factor (glycosylation-inhibiting factor), MMIF, Phenylpyruvate tautomerase
Gene Symbol
MIF
UniProt
Additional MIF Products
Product Documents for MIF Antibody (4E4) - BSA Free
Product Specific Notices for MIF Antibody (4E4) - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Related Research Areas
Citations for MIF Antibody (4E4) - BSA Free
Powered by Bioz
Customer Reviews for MIF Antibody (4E4) - BSA Free
There are currently no reviews for this product. Be the first to review MIF Antibody (4E4) - BSA Free and earn rewards!
Have you used MIF Antibody (4E4) - BSA Free?
Submit a review and receive an Amazon gift card!
$25/€18/£15/$25CAN/¥2500 Yen for a review with an image
$10/€7/£6/$10CAN/¥1110 Yen for a review without an image
Submit a review
Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Antigen Retrieval Protocol (PIER)
- Antigen Retrieval for Frozen Sections Protocol
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- Chromogenic IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Chromogenic Immunohistochemistry Staining of Frozen Tissue
- Detection & Visualization of Antibody Binding
- ELISA Sample Preparation & Collection Guide
- ELISA Troubleshooting Guide
- Fluorescent IHC Staining of Frozen Tissue Protocol
- Graphic Protocol for Heat-induced Epitope Retrieval
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Graphic Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- How to Run an R&D Systems DuoSet ELISA
- How to Run an R&D Systems Quantikine ELISA
- How to Run an R&D Systems Quantikine™ QuicKit™ ELISA
- ICC Cell Smear Protocol for Suspension Cells
- ICC Immunocytochemistry Protocol Videos
- ICC for Adherent Cells
- IHC Sample Preparation (Frozen sections vs Paraffin)
- Immunocytochemistry (ICC) Protocol
- Immunocytochemistry Troubleshooting
- Immunofluorescence of Organoids Embedded in Cultrex Basement Membrane Extract
- Immunofluorescent IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunohistochemistry Frozen Troubleshooting
- Immunohistochemistry Paraffin Troubleshooting
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Protocol for Heat-Induced Epitope Retrieval (HIER)
- Protocol for Making a 4% Formaldehyde Solution in PBS
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Fluorescent ICC Staining of Cell Smears - Graphic
- Protocol for the Fluorescent ICC Staining of Cultured Cells on Coverslips - Graphic
- Protocol for the Preparation & Fixation of Cells on Coverslips
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections - Graphic
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections - Graphic
- Protocol for the Preparation and Fluorescent ICC Staining of Cells on Coverslips
- Protocol for the Preparation and Fluorescent ICC Staining of Non-adherent Cells
- Protocol for the Preparation and Fluorescent ICC Staining of Stem Cells on Coverslips
- Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- Protocol for the Preparation of a Cell Smear for Non-adherent Cell ICC - Graphic
- Quantikine HS ELISA Kit Assay Principle, Alkaline Phosphatase
- Quantikine HS ELISA Kit Principle, Streptavidin-HRP Polymer
- R&D Systems Quality Control Western Blot Protocol
- Sandwich ELISA (Colorimetric) – Biotin/Streptavidin Detection Protocol
- Sandwich ELISA (Colorimetric) – Direct Detection Protocol
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: ELISA
- Troubleshooting Guide: Immunohistochemistry
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
Loading...