MIF Antibody (4E4) - BSA Free
Novus Biologicals | Catalog # NBP1-04319
![Western Blot: MIF Antibody (4E4)BSA Free [NBP1-04319]](https://resources.rndsystems.com/images/products/MIF-Antibody-4E4-Western-Blot-NBP1-04319-img0002.jpg "Western Blot: MIF Antibody (4E4)BSA Free [NBP1-04319]")
Scientific images may be resized, cropped, or adjusted for brightness or contrast for presentation purposes. These updates are limited to presentation and do not affect the underlying scientific interpretation of the data. Where available, additional source data may be provided upon request.
Key Product Details
Species Reactivity
Validated:
Human
Cited:
Human
Applications
Validated:
Immunohistochemistry, Western Blot, ELISA, Immunocytochemistry/ Immunofluorescence
Cited:
IF/IHC
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 kappa Clone # 4E4
Format
BSA Free
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Product Specifications
Immunogen
Recombinant human MIF (1-115 aa) purified from E. coli
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1 kappa
Scientific Data Images for MIF Antibody (4E4) - BSA Free
Western Blot: MIF Antibody (4E4)BSA Free [NBP1-04319]
Western Blot: MIF Antibody (4E4) [NBP1-04319] - The cell lysates of HL-60 was resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human MIF antibody (1:1,000). Protein was visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.![Immunocytochemistry/ Immunofluorescence: MIF Antibody (4E4) - BSA Free [NBP1-04319]](https://resources.rndsystems.com/images/products/MIF-Antibody-4E4-Immunocytochemistry-Immunofluorescence-NBP1-04319-img0004.jpg "Immunocytochemistry/ Immunofluorescence: MIF Antibody (4E4) - BSA Free [NBP1-04319]")
Immunocytochemistry/ Immunofluorescence: MIF Antibody (4E4) - BSA Free [NBP1-04319]
Immunocytochemistry/Immunofluorescence: MIF Antibody (4E4) [NBP1-04319] - Analysis of MIF in HeLa cells. The cell was stained at (1:100). The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue).![Western Blot: MIF Antibody (4E4)BSA Free [NBP1-04319]](https://resources.rndsystems.com/images/products/MIF-Antibody-4E4-Western-Blot-NBP1-04319-img0001.jpg "Western Blot: MIF Antibody (4E4)BSA Free [NBP1-04319]")
Western Blot: MIF Antibody (4E4)BSA Free [NBP1-04319]
Western Blot: MIF Antibody (4E4) [NBP1-04319] - The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human MIF antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.Lane 1.: Jurkat cell lysateLane 2.: THP-1 cell lysateLane 3.: HeLa cell lysateLane 4.: U937 cell lysate![Immunocytochemistry/ Immunofluorescence: MIF Antibody (4E4) - BSA Free [NBP1-04319]](https://resources.rndsystems.com/images/products/MIF-Antibody-4E4-Immunocytochemistry-Immunofluorescence-NBP1-04319-img0003.jpg "Immunocytochemistry/ Immunofluorescence: MIF Antibody (4E4) - BSA Free [NBP1-04319]")
Immunocytochemistry/ Immunofluorescence: MIF Antibody (4E4) - BSA Free [NBP1-04319]
Immunocytochemistry/Immunofluorescence: MIF Antibody (4E4) [NBP1-04319] - Analysis of MIF in Balb/3T3 cells. The cell was stained at (1:100). The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue).Applications for MIF Antibody (4E4) - BSA Free
Application
Recommended Usage
ELISA
1:100-1:2000
Western Blot
1:500-1:2000
Application Notes
Use in IHC reported in scientific literature (PMID:34514093).
Formulation, Preparation, and Storage
Purification
Protein G purified
Formulation
PBS (pH 7.4), 10% Glycerol
Format
BSA Free
Preservative
0.02% Sodium Azide
Concentration
1.0 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: MIF
Long Name
Macrophage Migration Inhibitory Factor
Alternate Names
EC 5.3.2.1, EC 5.3.3.12, GIFmacrophage migration inhibitory factor, GLIF, Glycosylation-inhibiting factor, L-dopachrome isomerase, L-dopachrome tautomerase, macrophage migration inhibitory factor (glycosylation-inhibiting factor), MMIF, Phenylpyruvate tautomerase
Gene Symbol
MIF
UniProt
Additional MIF Products
Product Documents for MIF Antibody (4E4) - BSA Free
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for MIF Antibody (4E4) - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Related Research Areas
Citations for MIF Antibody (4E4) - BSA Free
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Antigen Retrieval Protocol (PIER)
- Antigen Retrieval for Frozen Sections Protocol
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- Chromogenic IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Chromogenic Immunohistochemistry Staining of Frozen Tissue
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- ELISA Sample Preparation & Collection Guide
- ELISA Troubleshooting Guide
- Fluorescent IHC Staining of Frozen Tissue Protocol
- Graphic Protocol for Heat-induced Epitope Retrieval
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Graphic Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- How to Run an R&D Systems DuoSet ELISA
- How to Run an R&D Systems Quantikine ELISA
- How to Run an R&D Systems Quantikine™ QuicKit™ ELISA
- ICC Cell Smear Protocol for Suspension Cells
- ICC Immunocytochemistry Protocol Videos
- ICC for Adherent Cells
- IHC Sample Preparation (Frozen sections vs Paraffin)
- Immunocytochemistry (ICC) Protocol
- Immunocytochemistry Troubleshooting
- Immunofluorescence of Organoids Embedded in Cultrex Basement Membrane Extract
- Immunofluorescent IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunohistochemistry Frozen Troubleshooting
- Immunohistochemistry Paraffin Troubleshooting
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Protocol for Heat-Induced Epitope Retrieval (HIER)
- Protocol for Making a 4% Formaldehyde Solution in PBS
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Fluorescent ICC Staining of Cell Smears - Graphic
- Protocol for the Fluorescent ICC Staining of Cultured Cells on Coverslips - Graphic
- Protocol for the Preparation & Fixation of Cells on Coverslips
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections - Graphic
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections - Graphic
- Protocol for the Preparation and Fluorescent ICC Staining of Cells on Coverslips
- Protocol for the Preparation and Fluorescent ICC Staining of Non-adherent Cells
- Protocol for the Preparation and Fluorescent ICC Staining of Stem Cells on Coverslips
- Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- Protocol for the Preparation of a Cell Smear for Non-adherent Cell ICC - Graphic
- Quantikine HS ELISA Kit Assay Principle, Alkaline Phosphatase
- Quantikine HS ELISA Kit Principle, Streptavidin-HRP Polymer
- R&D Systems Quality Control Western Blot Protocol
- Sandwich ELISA (Colorimetric) – Biotin/Streptavidin Detection Protocol
- Sandwich ELISA (Colorimetric) – Direct Detection Protocol
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: ELISA
- Troubleshooting Guide: Immunohistochemistry
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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