MIST1 Antibody (6E8/A12/C11P1)

Novus Biologicals | Catalog # NBP2-22478

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human, Mouse

Cited:

Mouse

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen, Western Blot, Immunocytochemistry/ Immunofluorescence

Cited:

Immunohistochemistry, Immunohistochemistry-Frozen, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 6E8/A12/C11P1
View all MIST1 Primary Antibodies »

Product Specifications

Immunogen

Mouse Mist1, C-terminus residues 175-197.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for MIST1 Antibody (6E8/A12/C11P1)

Immunohistochemistry: MIST1 Antibody (6E8/A12/C11P1) [NBP2-22478]

Immunohistochemistry: MIST1 Antibody (6E8/A12/C11P1) [NBP2-22478]

Immunohistochemistry: MIST1 Antibody (6E8/A12/C11P1) [NBP2-22478] - Mist1 expression can be observed in mouse gastric chief cells(red). image is courtesy of customer review.
Immunohistochemistry-Paraffin: MIST1 Antibody (6E8/A12/C11P1) [NBP2-22478]

Immunohistochemistry-Paraffin: MIST1 Antibody (6E8/A12/C11P1) [NBP2-22478]

Immunohistochemistry-Paraffin: MIST1 Antibody (6E8/A12/C11P1) [NBP2-22478] - Staining of mouse pancreas tissues. The antibody gives nuclear staining in acinar cells but not in duct or islet cells.
MIST1 Antibody (6E8/A12/C11P1)

Immunohistochemistry: MIST1 Antibody (6E8/A12/C11P1) [NBP2-22478] -

DDX56 facilitates HCC growth in a MIST1-dependent manner. (A-B) Colony formation (A) and EdU assays (B) revealed that ectopic MIST1 expression in DDX56-silenced cells partially reversed the inhibitory effect on HCC proliferation. Scale bars: 100 um. (C) Western blot analysis of the PTEN-AKT axis in DDX56-silenced HCC cells showing that MIST1 overexpression reversed the effect of DDX56 on the PTEN-AKT axis. (D-E) DDX56 knockdown decreased liver tumor formation capacity of HCC cells in the orthotopic HCC implantation models, and the effect was reversed by simultaneously overexpressing MIST1. (D) Representative images showing mouse livers with tumor lesions. Red circles indicate the primary liver tumor. (E) Representative images of IHC staining of DDX56 and MIST1 in paraffin-embedded sections obtained from mouse orthotopic HCC models. Scale bars: 250 um (low magnification), 50 um (high magnification). Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/36168636), licensed under a CC-BY license. Not internally tested by Novus Biologicals.
MIST1 Antibody (6E8/A12/C11P1)

Immunohistochemistry: MIST1 Antibody (6E8/A12/C11P1) [NBP2-22478] -

RNA expression profiling identifies MIST1 as a DDX56-downstream target in the regulation of the PTEN-AKT axis. (A) A volcano plot constructed using P-values and fold-change to illustrate the differential downstream target genes between DDX56-silenced MHCC97H cells and control cells (|fold change| ≥2, P < 0.05). Red dots represent significantly upregulated genes while blue dots represent significantly downregulated genes. (B) The top 20 differential downstream genes from RNA-seq results were validated by RT-PCR in MHCC97H cells. (C-D) The mRNA and protein levels of MIST1 in both MHCC97H and Huh-7 cells were detected using RT-PCR and western blotting, respectively. (E) IHC staining shows the expression level of MIST1 in the subcutaneous tumor. Scale bars: 250 um (top), 50 um (bottom). (F) Representative images of DDX56 and MIST1 immunohistochemical staining in human HCC tissues (left). Correlation analysis between DDX56 and MIST1 protein expression in HCC tissues (right). Scale bars: 250 um (low magnification), 50 um (high magnification). (G) Effect of MIST1 knockdown on MHCC97H and Huh-7 cell proliferation was determined by colony formation assay. (H) The indicated protein was determined using a western blot analysis of MHCC97H and Huh-7 cells infected with MIST1-silencing lentivirus (shMIST1) or lentivirus control (shCont). (I) Western blot analysis of the PTEN-AKT axis in MIST1-overexpressed HCC cells, showing that PTEN overexpression reversed the effect of MIST1 on the PTEN-AKT axis. Cells were transfected with 3 ug of the indicated plasmids for 72 h. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/36168636), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Applications for MIST1 Antibody (6E8/A12/C11P1)

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:10-1:500

Immunohistochemistry

1:10 - 1:500

Immunohistochemistry-Frozen

1:10 - 1:500

Immunohistochemistry-Paraffin

1:10 - 1:500

Western Blot

1:100 - 1:500
Application Notes
The antibody gives nuclear staining in acinar cells but not in duct or islet cells. IHC reported in a verified customer review.

Reviewed Applications

Read 1 review rated 5 using NBP2-22478 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS with 1 mg/ml BSA

Preservative

0.05% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: MIST1

MIST1 plays a role in controlling the transcriptional activity of MYOD1, ensuring that expanding myoblastpopulations remain undifferentiated. Repression may occur through muscle-specific E-box occupancy by homodimers. Mayalso negatively regulate bHLH-mediated t

Alternate Names

basic helix-loop-helix domain containing, class B, 8, basic helix-loop-helix family, member a15, bHLHa15, BHLHB8, class A basic helix-loop-helix protein 15, Class B basic helix-loop-helix protein 8, class II bHLH protein MIST1, MIST1MIST-1, Muscle, intestine and stomach expression 1

Entrez Gene IDs

168620 (Human)

Gene Symbol

BHLHA15

UniProt

Q7RTS1 (Human)

Additional MIST1 Products

Product Documents for MIST1 Antibody (6E8/A12/C11P1)

Certificate of Analysis

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Product Specific Notices for MIST1 Antibody (6E8/A12/C11P1)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for MIST1 Antibody (6E8/A12/C11P1)

Customer Reviews for MIST1 Antibody (6E8/A12/C11P1) (1)

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  • MIST1 Antibody (6E8/A12/C11P1)
    Name: Feng Li
    Application: Immunocytochemistry
    Sample Tested: mouse stomach
    Species: Mouse
    Verified Customer | Posted 05/11/2017
    Mist1 expression can be observed in mouse gastric chief cells(red).
    MIST1 Antibody (6E8/A12/C11P1) NBP2-22478

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Protocols

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